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Sean Nash

Learning in Plants: Lessons from Mimosa pudica - 4 views

  • there is a lack of observational data on the behavior of plants
  • Observational research allows the researcher to establish baseline activity and response levels of the subject
  • Perhaps the first study of learning in plants was a habituation experiment reported by Pfeffer (1873) using the sensitive plant Mimosa. In this experiment, repeated mechanical stimulation of leaflets led to a decrease in sensitivity
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  • the question naturally arises as to whether Mimosa can discriminate between stimuli. This was answered in the affirmative by Holmes and Gruenberg (1965) whose experimental design included a drop of water and finger touch as stimuli
  • the results suggest that Mimosa can discriminate between stimuli
  • Thomas performed an experiment on classical conditioning in Mimosa that took advantage of the finding that, under field conditions, the leaflets of Mimosa slowly drop as dusk approaches and slowly rise at dawn (personal communication). In the experiment, the CS was turning on the light in the growth chamber and the US was touching selected leaflets. Thomas found that leaflets in the paired condition showed conditioning compared to a light only or alternating stimulation condition. This finding should be replicated with controls for pseudoconditioning.
  • Given the importance of the Gagliano et al. (2014) experiment and the failure to replicate some previous experimental results it is critical that the Gagliano et al. (2014) results be repeated by an independent laboratory.
  • In addition to Mimosa, habituation has been found in the carnivorous plant Drosera (sundew
  • Applewhite (1975) cites an experiment by Darwin reportedly showing habituation in the passion flower (Passiflora gracilis). During this experiment, when Darwin mechanically stimulated the passion flower tendrils, the tendrils no longer responded after 54 h of training.
  • Abramson et al. (2002) investigated the use of bioelectrical potentials as a method to explore plant behavior.
  • In an interesting article, Karpinski and Szechynska-Hebda (2010) discuss the intellect of plants from memory to intelligence. By studying recall, the researcher investigates a host of independent variables and that are solidly anchored to a set of dependent variables. This study focused on recall at the cellular level rather than as an externally observable behavior. The discussion of plant learning at various levels, from cellular to organismal and from different scientific fields is exactly what is advocated for in this article
  • There is also interest from behavioral scientists seeking to determine whether the similarities and differences in learning found among invertebrates and vertebrates could also be found in plants (Warden et al., 1940; Applewhite, 1975; Abramson et al., 2002; Guiguet, 2013).
  • The majority of early plant studies used the Sensitive plant (M. pudica). Mimosa has much to recommend it for learning studies. They are easy to maintain, much is known about its natural history, and they have a visible leaf closure response to external stimuli. However, there are drawbacks in the use of Mimosa, for example, it takes about 15 min for a leaf to recover (Holmes and Gruenberg, 1965) and not much is known about its genome in contrast to model species such as Arabidopsis thaliana whose entire genome is known.
  • In the following section we will focus on methods to develop studies utilizing habituation, sensitization, and classical conditioning techniques. Instrumental and operant conditioning will not be covered because at this time there are no Mimosa studies in these areas; although one can envision a situation where the opening and closing of a leaf can be detected electronically. Once detected, the response would produce a consequence such as an airpuff or changes in light intensity.
    • Sean Nash
       
      The remainder of this comprehensive article is to outline the main lines of research in this area:
  • Learning in Plants: Lessons from Mimosa pudica
    • Sean Nash
       
      This well-compiled article does a really nice job of outlining the history of study regarding plant behavior. It also suggests model organisms for study. Mimosa is a favorite of mine as their response is VERY rapid and quite visible for a plant! There are all sorts of feasible project ideas that could arise from this area of research.
Sean Nash

Study shows experimental evidence of an altruistic nature in small convict cichlid fish - 0 views

  • Study shows experimental evidence of an altruistic nature in small convict cichlid fish
    • Sean Nash
       
      This is a big favorite model organism for me and for a handful of former students. They are fascinating little fish with complex behaviors. However, the struggle is always: studies with vertebrate model organisms are always subject to far more scrutiny and paperwork. You have to prove beyond the shadow of a doubt that your study is not invasive, and you have a proper plan for their care before/during/after experimentation. I am actually switching out the back aquarium (that previously had African Cichlids) for these in August.
Sean Nash

Airplane Noise Exposure May Increase Risk of Chronic Disease | SPH - 0 views

  • “Prior research has shown that aircraft noise can elevate stress responses and disturb sleep, but there has been mixed evidence of any links with body mass index,” says study lead and corresponding author Matthew Bozigar, assistant professor of epidemiology at OSU and a former postdoctoral associate at SPH. “We were surprised to see a fairly robust link between aircraft noise and higher body mass index among women across the US.”
    • Sean Nash
       
      Again, this reminds me of the UCSD juncos and the increased stressors they must endure to live in an urban environment. However, we must remember that just because their emerging population in and around the UCSD campus is successful, it comes with a cost of shorter lifespans, increased mortality, etc. Very interesting stuff.
  • These new findings underscore the role of the environment on one’s risk of chronic disease.
  • Junenette Peters, associate professor of environmental health, and colleagues examined airplane noise exposure and self-reported BMI and other individual characteristics among nearly 75,000 participants living around 90 of the major US airports
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  • The team examined aircraft noise levels every five years from 1995 to 2010, using a day-night estimate (DNL) that captures the average noise level over a 24-hour period and applies a 10 dB adjustment for aircraft noise occurring at night, when background noise is low.
  • Although the team acknowledges that BMI is a suboptimal metric, the independent and strong association between more aircraft noise exposure and higher BMI that they observed is notable.
  • “We can only hypothesize about why we saw these regional variations, but one reason may relate to the era of regional development, building characteristics, and climate which may affect factors such as housing age, design, and level of insulation,” says Peters. “Regional differences in temperature and humidity may influence behaviors such as window opening, so perhaps study participants living in the West were more exposed to aircraft noise due to open windows or housing type, which allowed more noise to penetrate.”
  • Previous data suggest that Black, Hispanic, and low-income populations are disproportionately exposed to aircraft noise. The participants in the NHS study groups were primarily White and of mid-level socioeconomic status. 
  • “We need to study the potential health impacts of environmental injustices in transportation noise exposures alongside other environmental drivers of poor health outcomes” Bozigar says. “There is a lot more to figure out, but this study adds evidence to a growing body of literature that noise negatively impacts health.”
ryleighnyp

Real-time dialogue between experimenters and dreamers during REM sleep: Current Biology - 3 views

  • study by Strauss and Dehaene13Strauss M. Dehaene S. Detection of arithmetic violations during sleep.Sleep (Basel). 2019; 42: zsy232PubMed Google Scholar focused on electro- and magnetoencephalographic responses to spoken arithmetic equations (addition, multiplication, or subtraction operations).
  • study by Strauss and Dehaene13Strauss M. Dehaene S. Detection of arithmetic violations during sleep.Sleep (Basel). 2019; 42: zsy232PubMed Google Scholar focused on electro- and magnetoencephalographic responses to spoken arithmetic equations (addition, multiplication, or subtraction operations).
    • ryleighnyp
       
      Magnetoencephalography is a technique used to measure the magnetic fields produced by electrical activity in the brain. It is a non-invasive method that can provide high temporal and spatial resolution of brain activity. In the context of the research paper, the authors mention a study by Strauss and Dehaene that focused on electro- and magnetoencephalographic responses to spoken arithmetic equations during wakefulness and sleep. (Copilot)
  •  
    An interesting Sleep study about how scientist can communicate with people who are sleeping
katherine-medina

Cerebrospinal Fluid (CSF) Exchange with Artificial CSF Enriched with Mesenchymal Stem C... - 2 views

  • Moreover, toxicity of the CSF from patients affected by neurodegenerative diseases has been reported
  • Moreover, toxicity of the CSF from patients affected by neurodegenerative diseases has been reported
    • katherine-medina
       
      Good to note.
  • Moreover, no studies using biologically enriched-aCSF for exchanging the CSF have been published.
    • katherine-medina
       
      That makes me want to search hard for studies that do involve the transfer, however I would have to figure out a way to create an experiment around this base idea of artificial CSF.
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  • n humans, it was reported that filtration of the CSF was beneficial in Guillain-Barré syndrome [
    • katherine-medina
       
      interesting that filtering the liquid can help people.
  • . MSCs produce a variety of neurogenic, neuroprotective, and immunomodulatory agents [15,16,17,18,19,20,21], and have been shown to induce beneficial effects when transplanted in EAE-mice [22,23,24,25], stroke [26,27], traumatic brain injury [28], Parkinson’s disease [29], schizophrenia, and autism [30,31] as well as increased neurogenesis in adult mice
  • A beneficial effect was also demonstrated under Aβ neurotoxicity in PC12 cells
  • Artifical cerebrospinal fluid (aCSF) enriched with secretions of mesenchymal stem cells (MSCs) increases cell viability of PC12 and SH-SY5Y neuronal cell lines
  • While secretions of 2 days growing 10 or 100 K/mL MSCs in aCSF did not show an increase in PC12 cell viability
    • katherine-medina
       
      HMMM.... that is utterly fascinating the fact that after 2 days there seemed to be no change amongst the cell viability for both the 10 and 100 ml aCSF.
  • etions of 5 days growing MSCs in aCSF did show a significant increase in the PC12 cell viability relative to unenriched-aCSF treated cells
  • The principle of CSF exchange is similar to plasma exchange by plasmapheresis, which is in use for the treatment of autoimmune disorders
    • katherine-medina
       
      Did not know that it was similar to plasmapheresis.
  • while cell viability was reduced under Aβ, a significant increase in cell viability was noted in the enriched-aCSF treated cells
  • A similar trend of increased cell viability by enriched-aCSF treatment was noticed in SH-SY5Y cells exposed to H2O2, but without reaching a statistical significance
  • a significant increase in cell viability was noticed in the enriched-aCSF (
  • significantly suppressed in spleen lymphocytes treated with the enriched-aCSF compared to lymphocytes treated with (unenriched-) aCSF
    • katherine-medina
       
      This limiting of the lymphocytes is most likely a good thing under the guise of this paper because the suppression of lymphocytes likely helps with certain autoimmune disorders.
  • These results show that the “in/out” enriched-aCSF therapy was the most effective one, affecting both time of onset (EAE-control mice develop the disease at day 10 while the treated mice at day 14 post induction) and disease progression
  • Prolonged amelioration of EAE clinical symptoms during prolonged CSF exchange therapy: (in/out) enriched-aCSF protocol was more effective than (in) enriched-aCSF and (in/out) aCSF.
  • A trend of less demyelination in the LFB staining in the (in/out) enriched-aCSF treated- mice relative to the EAE-control mic
  • Our results show that elimination of endogenous CSF, and its replacement with MSC secretions enriched-aCSF ((in/out)-enriched-aCSF), delayed EAE onset and reduced the clinical score with indications of reduced axonal damage and demyelination.
  • in vitro and in vivo, has shown that MSCs can promote survival and axonal myelination in sensory dorsal root ganglia neurons and may be effective in non-inflammatory models of demyelination [
  • MSCs transplantation alone has been applied and tested with strong indications of beneficial effects in animal models of MS [22,23,24,25], stroke [26,27], traumatic brain injury [28], PD [29], schizophrenia, and autism
  • showing that local (intraventricular) transplantation of MSCs is more effective than intravenous administration
  • The feasibility of CSF exchange therapy reported here in the EAE model might possibly be applied to other neurodegenerative diseases of the CNS.
  • our approach of CSF exchange therapy could be beneficial for fully developed neurological diseases through a repeated application of the proposed CSF exchange protocol
    • katherine-medina
       
      A good thing to recognize and note for future reference.
  • 5. Conclusions
  •  
    This is a more recent study about the effects of artificial CSF infused with MSC in mice.
katherine-medina

Increased 3-O-sulfated heparan sulfate in Alzheimer's disease brain is associated with ... - 1 views

  • Genetic factors contribute substantially to the pathological process of AD as demonstrated by the fact that the genetic heritability in AD is in the range of between 60 and 80% (10).
  • however, whether the variants alter the level of 3-OST-1 enzyme activity to change the structure of HS remains unknown
    • katherine-medina
       
      Good to note what exactly the article remains unsure about
  • The synthesis of additional 13C-labeled calibrants to cover these three reported 3-O-sulfated tetrasaccharides will be the subject for future study.
    • katherine-medina
       
      LOOK INTO THIS PART CAYSE THEY ARE SAYING THAT THEY ARE NOT FULLY SURE
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  • the elevation of total HS and the percentage of the 3-O-sulfated domain in HS.
  • HS and tau revealed that the sugar mainly interacts with the proline-rich region 2, a repeat domain 2 in tau protein
  • Further analysis of the distribution of 3-O-sulfated HS from different areas of AD brains will be of interest and the subject for a subsequent study.
  • Experimental design
    • katherine-medina
       
      READ THIS IF THIS TOPIC COMES BACK UP AGAIN
  •  
    A interesting study about a sulfate that suggests that Alzheimer's is genetic
katherine-medina

Plants | Free Full-Text | Expression of a Stilbene Synthase Gene from the Vitis labrusc... - 0 views

  • Stilbenes are found in plants of different families, but the best studied stilbene is resveratrol (3,5,4′-trihydroxystilbene) isolated from grape plants.
  • Resveratrol synthesis in transgenic plants did not always render them fully resistant to one or another pathogen.
  • The aim of our investigation was to isolate a VlvSTS stilbene synthase gene from the hybrid grape V. labrusca x V. vinifera L. and obtain transgenic tobacco plants overexpressing the gene to study their resistance to a number of bacterial and fungal pathogens, among them bacteria as E. carotovora and fungi as B. cinerea, F. oxysporum, F. sporotrichioides, F. culmorum.
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  • by PCR. F
    • katherine-medina
       
      PCR is a method with which scientists are able to amplify a gene
  • Based on the results of the VlvSTS gene transcription analysis, seven transgenic tobacco lines were selected for further experiments.
  • Expression of the VlvSTS gene in tobacco plants led to a significant increase in the pollen grain size, but with a tendency to a decrease in the total number of pollen grains per anther
    • katherine-medina
       
      Too much of that gene leads to a decrease of fertility.
  • In all tests with E. carotovora bacteria, transgenic plants expressing the VlvSTS gene demonstrated a significantly higher resistance versus control plants
    • katherine-medina
       
      interesting
  • The leaf involvement was significantly smaller in transgenic plants expressing the VlvSTS gene.
  • Overexpression of the VlvSTS gene reduced the corolla pigmentation in transgenic plants.
    • katherine-medina
       
      Why did the overexpression of that gene reduce the corolla pgmentation.
  • binary vector
    • katherine-medina
       
      like a DNA transfer
  • showed a significant fertility reduction,
  • For the first time it was shown that plants expressing the VlvSTS gene had enhanced resistance to the bacterial pathogen E. carotovora subsp. carotovora B15.
    • katherine-medina
       
      Interesting that it took this long for it to be shown that this specific stilbene can fight against certain pathogens.
  • Interestingly, resistance correlated with the expression level of the stilbene synthase gene. In plants with a higher gene expression (L10 and L23), the area of Erwinia-caused leaf damage was in a range from 0 to 40% respect to the control, while the leaves of line L24 were affected up to 60–100%.
  • hile in transgenic lines, the lysis areas were significantly smaller, probably due to the synthesis of the phytoalexin resveratrol.
  • Some studies showed that Arabidopsis plants transformed with wild grape stilbene synthase genes responded to a pathogen attack by activating genes responsible for the synthesis of signaling molecules such as salicylic and jasmonic acids (SA and JA, respectively) [
  • Our data show a decrease in the total flavonoid content in the flower petals but not in the leaves of plants upon expression of the VlvSTS gene.
    • katherine-medina
       
      That seems rather odd.
  • We were the first to show that transgenic tobacco plants carrying the VlvSTS gene had a significantly larger pollen grain size and a smaller number of pollen grains per anther. At the same time, the number of fertile pollen grains decreased, especially in the plant line with the highest expression of the VlvSTS gene.
  • For example, in apple plants with the grape stilbene synthase gene Vst1 under its own promoter, the expression of the gene had no effect on pollen development
  • The study showed that the expression of a VlvSTS stilbene syntase gene in tobacco transgenic plants increases their resistance to bacterial pathogen E. carotovora. There was a significant reduction of disease symptoms after infection of leaves by grey mould fungus B. cinerea, but not to Fusarium fungi. We were the first to show that transgenic tobacco plants carrying the VlvSTS gene had a significantly larger pollen grain size and a smaller number of pollen grains per anther. The number of fertile pollen grains decreased, especially in the plant line with the highest expression of the VlvSTS gene. These changes resulted in a decreased weight of seed bolls in the transgenic tobacco lines.
    • katherine-medina
       
      Overall, this article is one that I may come back to depending on what path I go down when searching to develop a research topic further.
Sean Nash

Health Effects from Swimming Training in Chlorinated Pools and the Corresponding Metabo... - 4 views

  • Numerous epidemiological publications have associated the chlorination of pools with dysfunctions of the respiratory system and with some other diseases
  • Therefore, the health effects from swimming in chlorinated pools and the corresponding stress reactions in organisms are unclear. In this study, we show that although the growth and behaviors of experimental rats were not affected, their health, training effects and metabolic profiles were significantly affected by a 12-week swimming training program in chlorinated water identical to that of public pools.
    • katherine-medina
       
      This may prove to be a concerning fact that myself and others on the swim team swim rather consistently.
  • The epidemiological evidence for adverse health effects from swimming in chlorinated water primarily originate from studies concerning respiratory function and asthma, althoughVillanueva et al. reported a significant increased risk of bladder cancer for swimmers compared with nonswimmers [5].
    • katherine-medina
       
      Kinda scary to think about the fact that swimming could be hurting my lungs.
    • Sean Nash
       
      OK... this is all a very rare and interesting topic to me. I've never considered it. Of course, in thinking a bout this as a HS research topic, the fact that it centers around humans... is a feasibility issue. However, what sort of invertebrate models could we use in place? Would we be able to detect DBPs?
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  • Interestingly, the eyes and skin are the organs that are more directly affected than the lungs by the irritants in chlorinated water; instead of chlorination, training intensity, training frequency and choking on water may be the primary factors for lung damage induced by swimming.
  • he water for the EG was purified using a water purifier and then disinfected using calcium hypochlorite, similar to public swimming pools, whereas the water for the CG was only purified, not chlorinate
  • Therefore, the health effects from swimming in chlorinated pools and the corresponding stress reactions occurring in our bodies are unclear
  • In fact, similar symptoms, red and swollen eyes, dried skin and nasal mucosal congestion, always appear after humans swim in a chlorinated pool; however, the long-term (12 weeks) and high-frequency (5 days a week) of the experimental swimming training caused even worse symptoms in these experimental rats.
  • however, some unusual appearance changes appeared in the EG rats.
    • katherine-medina
       
      EG rats had the exposure of a public level of chlorine.
  • First, the skin around their eyes became increasingly red with the development of the experiment, and in the ending period of the experiment, bloodstains could be observed in the rims of most rats’ eyes.
    • katherine-medina
       
      Wow.
  • Third, their fur became increasingly dry and lackluster, and significant signs of hair loss were observed during the last month.
    • katherine-medina
       
      This doesn't sound good.
  • These results indicated that the fur, respiratory tracts and eyes of the EG rats were severely affected by chlorinated water, although their growth was essentially unaffected. According to our observations, the daily behaviors and sizes of the EG rats were normal, and their final body weights were even slightly heavier than those weights of the control group. Nevertheless, dried and lackluster fur, hair removal, bloody noses and eyes did occur in the EG rats and not to the CG rats.
  • A meta-analysis performed by Goodman et al. demonstrated that the association between asthma and swimming could only be confirmed among competitive swimmers and could not be confirmed among non-competitive swimmers
    • katherine-medina
       
      SO competitive swimmers are the ones with an association of asthma and pool water.
  • Additionally, an interesting phenomenon was observed by comparing the development of the bloody noses and bloody eyes. The bloody noses commonly appeared in the third and in the fourth week; however, approximately two weeks later, this symptom gradually disappeared. The significantly bloody eyes commonly appeared in the ending period of the experiment; however, this problem was becoming worse during the study, and no signs of improvement appeared. The bloody noses appeared first, suggesting that respiratory tracts may be more vulnerable to the irritants from the chlorinated water than eyes; the gradually disappearing symptom suggests that respiratory tracts may have some adaptability to chlorinated water possibly because of the protection from nasal mucous. In contrast, without the mucosal protection, the bloody eyes were becoming increasingly significant during the entire experiment, although this symptom appeared later than the bloody noses.
  • Hippurate is an inhibitor of glucose utilization in the muscle and in the kidneys, an inhibitor of glucose utilization in the kidneys and in the liver, a modulator of fatty acid metabolism, and a stimulator of ammoniagenesis
  • Three rats from the EG were found liver damage, indicating that the positive rate reached 18 percen
  • or instance, Tumasonis et al. reported that a significantly increased incidence of hepatic neoplastic nodules in female rats and a significantly increased incidence of hepatic adenofibrosis in both male and female rats were induced by the chronic ingestion of chloroform [15].
  • Representative hematoxylin and eosin staining (H&E, 400×) of liver sections.
    • katherine-medina
       
      The liver literally changed between the two groups.
  • . We suppose that the primary differences between the competitive swimmers and non-competitive swimmers are training intensity and frequency, not the pool water.
    • katherine-medina
       
      An interesting theory, but I wonder if it has been proved.
  • The first three principal components (PCs) explain 57.3% of the total variance of the raw data and are sufficient to present the differences between the EG and the CG
  • In Fig. 3, we can see that the majority of the differences between the EG and the CG are explained by PC 1 and PC 3.
  • which suggests that the 12-week swimming training in chlorinated water induced a significant change in the metabolic profiles of the experimental group rats.
  • As an antioxidant, taurine can directly scavenge hypochlorous acid (HClO) and prevents changes in membrane permeability due to oxidative impairment
  • The duration period from the entry into water until the rats reached exhaustion was significantly shorter for the EG rats compared with the CG rats (29.74±11.50 vs. 39.15±9.85 minutes, p<0.05)
    • katherine-medina
       
      Rats in chlorine swam ten minutes less on average.
  • Chlorine is a necessary element for our bodies, and nontoxic.
    • katherine-medina
       
      I did not know that.
  • In summary, the 12-week swimming training in chlorinated pool water did induce some disease symptoms, an impairment of training effects and a significant change in the metabolic profiles, although the growth and behaviors of the experimental animals were not affected
  • Thus, the eyes and skin might be the organs that require greater attention for permanent damage.
  • Symptoms found in the liver and lung sections indicate that the liver is most likely the most possible target organ of DBPs, and training intensity, training frequency and water choking may be the primary factors for lung damage induced by swimming, instead of chlorination. The result of the swimming capacity test showed that training effects were significant affected by chlorinated water through perturbing the TCA cycle and oxidative phosphorylation.
  •  
    A really cool article about how chlorine can affect the bodies of mice. (An article I will never be able to forget about due to it being similar to my situation.)
  •  
    A really cool article about how chlorine can affect the bodies of mice. (An article I will never be able to forget about due to it being similar to my situation.)
katherine-medina

Effects of experimental increase in insulin-like growth factor 1 on feather growth rate... - 0 views

  • In mammals, IGF-1 also induces the proliferation of hair follicles and inhibits apoptosis, and therefore helps to keep them longer in the active (so-called anagen) phase and delay their transition to the regressive (catagen) phase
  • while a recently published study indicated a positive relationship between plumage quality, feather vane length and IGF-1 in a passerine species
  • Detailed quantification of the moulting stage was achieved by scoring the moult of the primary wing feathers and tail feathers on a scale of 0–5, using the standard protocol for recording the progress of feather growth as suggested by the British Trust for Ornithology and further described in
    • katherine-medina
       
      I had no idea one could predict when a bird will molt.
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  • As a result, 2 weeks after treatment, IGF-1-treated birds were in a more advanced stage of moult than controls
    • katherine-medina
       
      Interesting
  • In the longer term, IGF-1 treatment altered the intensity of moult: 2 weeks after treatment, control birds were moulting fewer feathers than at the start of the experiment, while IGF-1-treated birds showed the opposite pattern and increased the number of feathers being moulted simultaneously, albeit with substantial individual variation
  • We manipulated IGF-1 levels using an injection of poly-(lactic-co-glycolid acid) (PLGA) microspheres prepared by S.V.-K. and B.A.G. as described previousl
  • For example, stressors may cause a decrease in circulating IGF-1 levels (Tóth et al., 2018), and it may not be beneficial if that would affect the growth rate or the size of the developing flight feathers.
  • The fact that the growth of the tail feathers was also unaffected by the manipulation is more surprising as they are sexually selected ornaments in this species
    • katherine-medina
       
      Wow, I wonder why it had no effect on the growth of the tail feathers.
  • espite the robust lack of effect on feather growth speed, IGF-1 treatment maintained or even increased the number of feathers moulted simultaneously within 2 weeks, while the number of simultaneously growing feathers dropped in controls within the same period
    • katherine-medina
       
      Cool
  • In contrast to hair, the number of feather follicles that produce wing and tail feathers is small and shows no variation within species
    • katherine-medina
       
      I had no idea.
  • Some species, however, moult several wing and tail feathers simultaneously, which also applies to the bearded reedling
    • katherine-medina
       
      Why do some species of birds molt more feathers at the same time than others.
  • . Protein synthesis in tissues, for example, is partly promoted by and regulated through IGF-1 and this might also be of importance during the growth of feathers, in particular considering the increased demand for proteins during moulting
  • The interaction of corticosterone and IGF-1 has been suggested to predict fitness in growing songbirds (Lodjak et al., 2016). Corticosterone levels are known to have a seriously detrimental effect on growing feathers
    • katherine-medina
       
      How does corticosterone and IGF-1 interact?
  •  
    A study about the effect of insulin on the growth of feathers.
katherine-medina

Anti-Oxidative and Anti-Inflammatory Effects of Ginger in Health and Physical Activity:... - 1 views

  • the rhizome
  • Ginger has staring potential for treating a number of ailments including degenerative disorders (arthritis and rheumatism), digestive health (indigestion, constipation and ulcer), cardiovascular disorders (atherosclerosis and hypertension), vomiting, diabetes mellitus, and cancer.
    • katherine-medina
       
      It has the potential for it, so there may not be many studies on these different things that ginger can supposedly do. So it makes me Infinitely more curious to look and see if there are other studies that actually have information about these said claims.
  • Furthermore, it has antimicrobial potential as well which can help in treating infectious diseases.[2
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  • The bioactive molecules of ginger like gingerols have shown antioxidant activity in various modules
  • Inflammatory disorders such as gastritis, esophagitis, and hepatitis, which are caused not only by infectious agents such as viruses, bacteria, and parasites but also by physical and chemical agents like heat, acid, cigarette smoke, and foreign bodies, are recognized as risk factors for human cancer
  • This study aimed to review the current evidence on ginger effects as an anti-inflammatory and anti-oxidative.
  • es.[2
  • 6-Shogaol has exhibited the most potent antioxidant and anti-inflammatory properties in ginger, which can be attributed to the presence of alpha, beta-unsaturated ketone moiety
  • Furthermore, feeding ginger to rats at 1% w/w during administration of malathion
    • katherine-medina
       
      Wow, so they fed the rats pesticides than ginger
  • so it can possibly value in treatment of Parkinson's disease.[17]
    • katherine-medina
       
      Really cool
  • This effect was improved by a treatment with 1% dietary ginger 1 month in rats which suggest that ginger may have protective role against the ethanol induced hepatotoxicity.
  • could be useful in preventing acute liver injury
  • Habib et al. showed that ginger extract can reduce the elevated expression of NFκB and TNF-α in rats with liver cancer
  • howed that gingerols can inhibit LPS-induced COX-2 expression while shogaol containing extracts has no effect on COX-2 expression
  • Ginger and its bioactive molecules are effective in controlling the extent of colorectal, gastric, ovarian, liver, skin, breast, and prostate cancers
    • katherine-medina
       
      Wow it can do a lot.
  • They observed that [6]-gingerol inhibits TRAIL-induced NF-κB activation by impairing the nuclear translocation of NF-κB, suppresses cIAP1 expression, and increases TRAIL-induced caspase-3/7 activation
  • Inhibition of angiogenesis in the mouse skin is the mechanism of ginger for treating of skin cancer.
  • However, ginger significantly lowered blood glucose, serum total cholesterol, LDL, VLDL, and triglycerides, and raised HDL in hyperglycemic rats, in models that are diabetic, deficient in the apolipoprotein E gene or those that have been fed a high lipid diet.
  • ethanolic extract of ginger reduced body weights and levels of glucose, insulin, total cholesterol, LDL cholesterol, triglycerides, free fatty acids, and phospholipids in high-fat diets.
  • CONCLUSIONS
    • katherine-medina
       
      Overall a good synopsis on what ginger can do.
katherine-medina

Anti-inflammatory effects and possible mechanism of action of lupeol acetate isolated f... - 0 views

  • The latex collected from its stem bark is used for several purposes including anti-inflammatory properties and presents among its bioactive constituents the pentacyclic triterpene lupeol.
  • administered with LA,
    • katherine-medina
       
      they were trying to see if it could work as a prevenatitive treatment
  • carrageenan and dextran,
    • katherine-medina
       
      This causes inflammation
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  • the effect of a very low dose of LA (0.1 mg/kg) was potentiated by the same dose of pentoxifylline (PTX), a known TNF-alpha inhibitor. L
    • katherine-medina
       
      Essentially once they put pentoxifylline into the rat the LA activated.
  • The anti-inflammatory effect of LA probably involves the opioid system, as indicated by the complete blockade of the opioid antagonist naloxone
    • katherine-medina
       
      So, the LA helped the body with inflammation due to its interaction with the opioid system.
  • rich in triterpenes
  • Carrageenan-induced mice paw edema
    • katherine-medina
       
      Cool to understand that these next two paragraphs are about how to induce inflammation
  • LA injected 30 min before carrageenan significantly decreased the carrageenan-induced neutrophil migration in a dose-dependent manner. The LA inhibitory effect against carrageenan-induced migration was about 52, 79 and 90%, at the doses of 1, 10 and 20 mg/kg, i.p., respectively
  • LA (10, 25 and 50 mg/kg, i.p.) reduced both phases of the formalin test, and the results were significant at the two higher doses. However, the effects were mainly on the 2nd phase with 61% inhibition, whereas the 1st phase was inhibited by 41% at the LA dose of 50 mg/kg, i.p. The naloxone pretreatment completely reversed the LA effect, in the 1st and 2nd phases, indicating the participation of the opioid system in LA antinociceptive and anti-inflammatory actions.
  • On the other hand, while no significant enzyme release was observed with LA at the concentrations of 1, 10 and 25 μg/mL, a small but significant LDH release (around 2 times) was detected with the higher LA concentration (50 μg/mL), probably related to the presence of 0.2% Tween 80.
  • The results show that LA at the concentrations of 50, 100 and 200 μg/mL presents no radical scavenging capacity. On the contrary, vitamin E used as the reference drug significantly decreased the absorbance value, as related to controls
    • katherine-medina
       
      LA can sadly not kill the free radicals that tend to disrupt and kill DNA and other parts of the cell.
  • In the carrageenan-treated groups pretreated with LA (50 mg/kg, i.p.) or indomethacin (10 mg/kg, i.p.), there were significant reductions of iNOS expressing cells.
  • Lupeol is found in several other species and its antinociceptive and anti-inflammatory activities have been already demonstrated [24–28]. It is accepted that the anti-inflammatory property of lupeol often accompany its immune modulatory and anti-tumor action
  • lupeol acetate presents an anti-inflammatory activity by regulating TNF-alpha and IL-2 specific mRNA, besides upregulating the synthesis of IL-10 mRNA [31].
  • In the present work, we showed that lupeol acetate (LA, 93.2% purity) isolated from the H. drasticus latex presented a potent anti-inflammatory action, in several models of inflammation in mice
  • These authors concluded that lupeol possessed an anti-inflammatory activity which is probably related to its ability to prevent the production of pro-inflammatory mediators, such as TNF-α and IL-1β.
  •  
    This study is a good one to go back to if you are curious about alternative types of anti-inflammatory plants.
  •  
    The carrageenan here (and the fact that you seem interested in the biochemical realm) made me think of a supplement my mom recently asked me to scope out for her (Arteriosil). It also contains a seaweed extract (rhamnan sulfate) Her doctor was recommending it for macular degeneration. Here is the product: https://shop.calroy.com/product/arterosilhp/?gclid=Cj0KCQjw7aqkBhDPARIsAKGa0oKoQchIRSlzL1_PikNwT71f1BmUVgbIM7sXUQS_lJKaGVSCT4O5R7EaAmaUEALw_wcB Here is one study from the NIH: https://pubmed.ncbi.nlm.nih.gov/32344720/
katherine-medina

Full article: Effect of Dandelion root extract on growth performance, immune function a... - 0 views

  • From days 12 to 14 and days 26 to 28, approximately 100 g of faeces was collected from each pen for 3 days and the faecal samples were stored at −20°C. The 3-d faecal collection was pooled by pen and then dried at 65°C for 72 h.
  • From days 12 to 14 and days 26 to 28, approximately 100 g of faeces was collected from each pen for 3 days and the faecal samples were stored at −20°C. The 3-d faecal collection was pooled by pen and then dried at 65°C for 72 h.
    • katherine-medina
       
      Kinda gross, but interesting to know how and when the collected the "feces."
  • There were no differences in the ATTD of GE, CP, NDF and ADF among the three treatments.
    • katherine-medina
       
      Interesting that there was no difference between the three groups in that category.
  • ...10 more annotations...
  • uring days 15–28, pigs in the CHE group had lower (P < .05) diarrhoea frequency compared with those in the NC treatment, and dietary CHE supplementation decreased the diarrhoea frequency by 63.62% and 57.82% compared with the NC and PC groups, respectively
    • katherine-medina
       
      So the Chinese herbal extract did a better job at reducing the diarrhoea frequency by 63.62 and 57.82% in comparison to the NC and PC groups.
  • Furthermore, pigs in the CHE group had greater (P < .05) formic acid, propionate and butyrate concentrations in faeces compared with pigs in the PC group, and had greater (P < .05) formic acid, butyrate and valerate concentrations in faeces compared with pigs in the NC group
    • katherine-medina
       
      Cool to note that the CHE group had a lower lactic acid concentration in comparison to the PC group.
  • There were no differences in the concentrations of IgA, IgG, IgM, GSH-PX, MDA, TNF-α, IL-4 and IL-1β among the three treatments.
  • Moreover, the concentration of IL-6 in serum in pigs fed the CHE diet was greater (P < .05) than those fed the NC diet.
  • Pigs in the CHE group showed increased relative abundance of Sytrophococcus on genus level, and decreased (P < .05) relative abundance of Clostridiaceae_1
  • In addition, relative abundances of genus Selenomonas, Alloprevotella, Prevotella_2, Rjkenellaceae, and Kitasatospora all increased in faecal samples when pigs fed the PC diet.
  • CHE supplementation decreased the diarrhoea frequency of pigs compared with diets with or without antibiotics supplemented. Some previous studies also reported the positive effects of dietary Chinese herbs supplementation on diarrhoea frequency of weaned pigs
  • he increased concentrations of immune markers in serum and faecal SCFAs in weaned pigs consumed CHE in our study may explain the positive effects of CHE supplementation on diarrhoea frequency and nutrient digestibility
  • but decreased the incidence of diarrhoea and increased digestibility of some nutrients.
  • In addition, CHE supplementation improved faecal SCFAs concentrations and immune function of weaned pigs, and shaped faecal microbial community in weaned pigs. As a result, the traditional Chinese herb medicine, CHE, could act as a potential alternative to the antibiotics such as aureomycin used in weaned pig diets.
    • katherine-medina
       
      I do quite enjoy this closing statement, so in summary CHE works to decrease diarrhoea in weened pigs.
  •  
    An article showing that pigs who ate CHE had a significant decrease in diahrroea.
  •  
    Super interesting. Just remember that experimentation on mammals where diet is altered, etc... is a super tightrope walk. It is almost impossible to get those approved. You would have to really dig into the ISEF rules on that. However, you can always think about ways to transfer ideas and concepts down to a "lower" animal model for many studies.
katherine-medina

Frontiers | Cerebrospinal Fluid Pressure-Related Features in Chronic Headache: A Prospe... - 0 views

  • magnetic resonance
  • magnetic resonance
    • katherine-medina
       
      A non-invasive imaging software
  • A respondent to medical treatment (good outcome) was considered a subject who achieved a 50% or greater reduction in number of headache days per month.
  • ...14 more annotations...
  • The pressure transducer (Transducer kit, Edwards Lifesciences, Irvine, CA, USA) was attached to the hub of needle via a 10-cm long flexible tube without loss of CSF.
  • 93 had abnormal CSF pressure pulsations, with opening pressures ranging from 206 to 385 mm
  • . Group 1 comprised 55 patients, each with an opening pressure below 200 mm H2O
  • Group 2 comprised 56 patients, each with a slight elevation of CSF opening pressure (mean = 231.8
  • Group 3 included 37 patients, all with both elevated CSF opening pressure (mean = 282.2 mm H2O,
  • n Group 3, patients with severe abnormal pressure pulsations and mean pressure >250 mm H2O had the most severe headaches (highest scores on VAS and MIDAS) and associated symptoms (high frequency of: nocturnal headache, postural headache, photopsias, transient visual obscurations).
  • In Group 2, patients with less severe abnormal pressure pulsations and mean pressure >200 mm H2O, had less severe symptoms; in Group 1, patients who had no abnormal pressure pulsations and mean pressures below 200 mm H2O had primary chronic headache disorder (Figure 1).
  • . All of the subjects in the control group, all with opening pressures below 200 mm H2o and normal CSF pressure pulsations, had no headache.
    • katherine-medina
       
      So essentially if the CSF pressure was too low the patient had chronic headache disorder, but if the patients had a higher level of pressure they would suffer from the most painful headaches. (This occurring as nocturnal headaches, postural headaches, and transient visual obstructions.
  • An improvement in the intensity of pain and frequency of headaches was observed following CSF removal in all patients with ICH
  • 69 (74%) out 93 patients achieved ≥50% reduction in headache days.
  • Our results provide evidence that nocturnal and postural headache, and abnormal pressure pulsations are the most common pressure-related features of isolated high CSF pressure in patients with chronic headache
  • o explain this mimicry, we hypothesize that abnormal pulsations in CSF pressure may trigger the same mechanism that cause the pain in a primary headache attack. The mimicry thereby emerges as a variation of pre-existing primary headache; as such, it could be considered a triggered headache rather than a secondary or primary one.
  • The pathogenesis of these abnormal pulsations in CSF pressure (B waves and plateau waves) may be clarified by reference to the theory that CSF pressure is derived from the circulation of cerebral blood and cerebrospinal fluid
    • katherine-medina
       
      Hmmm... I had no idea that there is a theory that states that CSF pressure is derived from the circulation of blood. (I might wanna look into the further)
  • This suggests that CSF pressure assessment by measuring the height of the fluid column in the manometer line connected to a spinal puncture needle may be misleading in the case of headache sufferers.
  •  
    A good study to look at when looking at possible causes of headaches, and more information about CSF.
katherine-medina

The-ethics-of-experimenting-with-human-brain-tissue.pdf - 0 views

  •  
    A study that raises interesting questions on the ethics of growing human brain tissue.
Grant Reed

Study Finds Carrying Pollen Heats Up Bumble Bees, Raising New Climate Change Questions - 3 views

  • This new understanding of active bumble bee body temperatures raises questions about how these species will be impacted by a warmer world due to climate change.
    • Grant Reed
       
      Trying out the highlight feature. This might be an interesting research topic.
katherine-medina

The chemical composition and antioxidant properties of common dandelion leaves compared... - 1 views

  • sea buckthorn leaves exhibited a significantly higher level of antioxidant activity as measured by ABTS
  • . Dandelion leaves were richer in tocopherols, thiamine, riboflavin, and niacin while the sea buckthorn leaves contained higher levels of l-ascorbic acid.
  • aw dandelion are partly related to the antioxidant properties of some of its components;
    • katherine-medina
       
      Why haven't they done more studies on this specific part about dandelions?
  • ...5 more annotations...
  • The present study will also be helpful with regard to the standardization of bioactive content in plant materials.
    • katherine-medina
       
      Cool, cool, although I was hopping that their goal was something slightly different.
  • polyphenols: flavonoids, isoflavones, anthocyanins, and catechins
  • Sea buckthorn
  • Plants contain small amounts of lead, which may significantly increase when exposed to lead contamination in the soil and air; however, lead was not detected in the examined leaves of dandelion and sea buckthorn.
    • katherine-medina
       
      Did not know that.
  • neutralize ABTS
    • katherine-medina
       
      So essentially the higher the antioxidant concentration the more the color of the ABTS changes.
  •  
    This article helped me understand a few of the different antioxidants that dandelions, and sea buckthorn.
katherine-medina

Dandelion root extract affects colorectal cancer proliferation and survival through the... - 0 views

  • of an aqueous dandelion root extract
  • caspase-8 activation was not essential for the induction of cell death in colon cancer cells as an inhibition of caspase-8 activation did not alter the cytotoxicity of DRE
  • We have been able to identify four pharmacologically active components, α-amyrin, β-amyrin, lupeol and taraxasterol, in two out of the six bioactive fractions, but the anti-cancer activities of the individual compounds were not as strong as that of the unfractionated DRE indicating, clearly, the benefits of using the whole extract.
  • ...16 more annotations...
  • which might represent a novel non-toxic alternative to conventional cancer therapy available today.
  • These results clearly indicate that dandelion root extract can inhibit the ability of colorectal cancer cells to migrate and invade, and therefore metastasize to secondary locations.
    • katherine-medina
       
      Wow. I like to see that in 3 different studies DRE was proven to selectively pick the cancer cells, and ignore the normal cells.
  • morphological differences in tissue slices between the control untreated and the DRE treated group
  • aken together, these results established that systemic oral intake of the DRE was safe and its anti-cancer efficacy should be further investigated.
    • katherine-medina
       
      I love the fact that they yet again state that I should look more into this topic.
  • , but the DRE treatment efficiently suppressed the growth of both p53 WT and p53 mutant tumors in-vivo (Figure 4B – 4C)
    • katherine-medina
       
      great, they suppressed the growth of the tumors.
  • with no difference between the control and DRE treated samples of NCM460
    • katherine-medina
       
      I would not have thought that the mitochondria would be left alone by the drug.
  • Others suggest that following activation, caspases re-localize to the mitochondria, where they interact with other pro-apoptotic proteins during the progression of apoptosis [15]. A third option, put forward by Qin and colleagues, suggests that inactive caspases are kept in the mitochondria, but following apoptotic stimuli and activation, they are released from the mitochondria into the cytoplasmic peri-nuclear space [
  • The results showed a progressive destabilization of the mitochondrial membrane following the DRE treatment, which was observed as early as 30 minutes post treatment (Figure ​6C). Pro-caspase-8 (green) was localized in the mitochondria (red) in control untreated cells; however, following the DRE treatment, activated caspase-8 was released from the mitochondria into cytoplasmic space, as indicated by the dispersed green fluorescence (Figure ​6C
    • katherine-medina
       
      Pro-capase-8 helps to fight against the cancer
  • suggesting that in HT-29 colorectal cancer cells the DRE-induced cell death was caspase-8 independent.
    • katherine-medina
       
      So essentially caspase 8 had nothing to do with it
  • We observed a decrease in the viability of cells treated with α-amyrin, with 10 μM as the most effective concentration.
    • katherine-medina
       
      Hmm. the beginnings of narrowing down what it is about the plant that is able to fight cancer.
  • However, these results indicate that DRE and its anti-cancer components must be absorbed and circulated, in order to reach the site of the tumor (in order to inhibit tumor growth).
    • katherine-medina
       
      So it needs to be drank, or swallowed in a pill form to work.
  • , we confirmed the vulnerability of cancer cell mitochondria by showing that the DRE treatment led to a decrease in the mitochondrial membrane potential and increase in ROS levels in the isolated mitochondria.
  • caspase-8 specific inhibitor, IETD-fmk, did not change the DRE response in these cells. This was in contrast to our previous study in leukemia and pancreatic cancer cells
    • katherine-medina
       
      For each different cancer a new slightly different result is produced
  • he pro-apoptotic genes including Caspase-1, Interferon gamma and the TNF ligands and receptors, were up-regulated in HT-29 cells, prior to the apoptosis induction, while the same genes were down-regulated in NCM460 cells.
  • Previous findings show that taraxasterol has anti-inflammatory and chemopreventive activit
  • suggesting its importance in the anti-cancer activity of dandelion root extract, especially on the expression levels of COX-2. Additionally, we show that 10 μM lupeol is not very effective on its own
  •  
    Yet another article about how DRE can fight against cancer.
katherine-medina

The Efficacy of Dandelion Root Extract in Inducing Apoptosis in Drug-Resistant Human Me... - 0 views

  • 2. Materials and Methods
  • 2. Materials and Methods
    • katherine-medina
       
      If I plan on doing some sort of experiment with Dandelion root, I will likely need to come back and look at how this study did it.
  • After a long exposure of 96 hours, NHFs did not exhibit any reduction in cell viability
    • katherine-medina
       
      Wow, so even after 3 days the Dandelion Root did nothing to the NHF aka. normal human cells.
  • ...15 more annotations...
  • With DRE having proven its efficacy in successfully killing this aggressive, chemoresistant form of skin cancer, DRE toxicity on normal cells had to be evaluated
    • katherine-medina
       
      Always remember to hav e a control.
  • DRE was found to reduce cell viability in a dose-dependent fashion, over time, in A375 melanoma cells as was measured by WST-1 assay. Based on metabolic activity of A375s, it was confirmed that treatment at 2.5 mg/mL DRE resulted in ~50% reduction in cell viability against control within 24 hours (Figure 1(a))
  • Higher doses were then used and a response was observed at a concentration of 10 mg/mL (
    • katherine-medina
       
      For different types of melanoma a different amount of DRE is needed.
  • Typical apoptotic morphology was observed in G361 cells treated with DRE starting at 5 mg/mL concentrations for 72 hours
  • . However, there has been little scientific advancement made in this field with regard to the effect of dandelion root extract on cancer, and even more so on chemoresistant, human malignant melanoma skin cancer.
    • katherine-medina
       
      I do so love it when the author identifies the fact that there is so few research papers about DRE.
  • ) is more than a worthy chemopreventative, it is fast-acting, nontoxic, and therefore specific in its targeting of human melanoma cancer cells, making it a valuable chemotherapeutic. We have investigated the induction of apoptosis in human malignant melanoma cells and observed its long-term effects in human melanoma cancer.
    • katherine-medina
       
      alrighty then.
  • We are yet to determine the effect of each of the individual components (such as the family of triterpene alcohols and phenolic acids—found in the roots—and cinnamic acids, flavinoids and coumarins—that are found in the leaves
    • katherine-medina
       
      Maybe I could look into the specific component that kills the cancer, so that in future years after I had figured this out I could put it into practice.
  • Given that DRE has traditionally been used naturopathically for a variety of ailments, we assume that it would be relatively nontoxic to healthy cells. Our results show that the Normal Human Fibroblasts (NHFs) (which were treated at a low population doubling where NHFs have the best proliferation rate) and Peripheral Blood Mononuclear Cells remained unaffected and healthy after a 96-hour and 48- hour exposure to DRE, respectively (Figures 2(a)–2(d)).
  • Lupeol,
    • katherine-medina
       
      What is Lupeol. (I should probably look into that.)
  • taraxasterol
  • More importantly, an increase in ROS production indicates prooxidant behaviour of DRE on cancer cell mitochondria, which is contrary to the antioxidant convictions of traditional medicine and previous studies on Taraxacum extracts citing reductions in NO, ROS, RNS, and COX-2 [10, 11] in mouse macrophages.
    • katherine-medina
       
      That is very important and interesting.
  • There are two main points that must be stated here: firstly, that noncancerous cells are unaffected by DRE treatment, and secondly, melanoma cells retain the signals to commit suicide long after DRE has been removed from the system
    • katherine-medina
       
      Good to restate.
  • Metformin acts as a metabolism interfering compound that debilitates cancer cells, and the case of G361-resistant melanoma cells, combining DRE with metformin reduces cell viability at even lower doses (Figures 9(a) and 9(b)).
  • By 48 hours, human melanoma A375 cells uncharacteristically showed susceptibility to apoptosis induction by DRE
  • We believe that this nontoxic extract can undergo precipitous translation from bench top to bedside, with dandelion products that are already commercially available in the form of tea and supplements.
  •  
    Essentially it is an article that figured out that DRE can induce apoptosis in melanoma cells, and it also proved that DRE is non-toxic to normal human cells.
katherine-medina

In Vitro and In Vivo Evaluation of the Effectiveness and Safety of Amygdalin as a Cance... - 0 views

  • ]. Approximately 80% of all medications approved by the FDA in the last three decades have been derived from natural sources
  • Transdermal drug delivery is a promising route for cancer treatment compared with the oral route due to its low side effects and improved efficacy and selectivity
  • All ALN formulations containing DDP exhibited a higher percent of EE and smaller particle size and PDI than those that did not have DDP at the molar ratio investigated.
    • katherine-medina
       
      If I am to do my research project over the affects of amygdalin patches on cancer, I will need to keep this section of text in mind.
  • ...9 more annotations...
  • The optimum ALN gel formulation reduced mean relative carcinoma volume (MCV) at a higher rate (p < 0.05) compared with free amygdalin solution and free tamoxifene suspension.
  • that the optimum ALN gel formulation reduced mean relative carcinoma volume (MCV) compared with the DMBA control.
    • katherine-medina
       
      reduced carcinoma in comparison to control group
  • . The value of the zeta potential indicated a negative surface charge, which is considered advantageous for transdermal drug delivery and for electrostatic stabilization due to the electrostatic repulsions between vesicles
    • katherine-medina
       
      Good to note.
  • 3. Materials and Methods3.1. MaterialsAmygdalin was attained from Nature’s Only Choice Company (Tbilisi, GA, USA). Sigma Aldrich (Agitech Company, Cairo, Egypt) provided Tween 60, Span 60, cholesterol, 7, 12-dimethylbenz[a] anthracene (DMBA), triethanolamine, and dihexadecyl phosphate. Carbopol 934, methanol, acetone, and chloroform were attained from Corner-Lab Company (Cairo, Egypt).
  • Histological examination of the oral tamoxifen suspension treated group (Figure 7C) revealed the presence of hyperkeratosis and acanthosis in the surface epithelium of the epidermis with signs of a diffuse inflammatory response and edema in the dermis and sub-cutaneous tissue.
  • Histological examination of the optimum ALN gel treated group (Figure 7F) showed clearly healed skin with normal covering epithelium and marked improvement in all signs of the epidermis and dermis that were better than those of the oral amygdalin solution. These results confirmed the effectiveness of amygdalin loaded niosomes gel as a cancer therapy in vivo.
    • katherine-medina
       
      Cool, so they tested and confirmed that ALN gel does work to treat cancer of the skin.
  • Histological examination of the optimum ALN gel-treated group (Figure 8B) showed clearly healed skin with normal covering epithelium.
  • The group treated with plain niosomes gel showed MCV nearly similar to that of the DMBA control group.
    • katherine-medina
       
      SO the niosome gel doesn't do much.
  • The optimum ALN gel enhanced the permeation of amygdalin into deep skin layers and showed significant anti-tumor activity compared with oral tamoxifen.
    • katherine-medina
       
      I think that Amygdalen could be one of my research topics for this upcoming year.
  •  
    A really cool study that showed that Amygdalin in a gel form can prove to be very sucsessful at healing tumors from the epidermis.
katherine-medina

IJMS | Free Full-Text | Antioxidant Versus Pro-Apoptotic Effects of Mushroom-Enriched D... - 0 views

  • In addition, the gut microbiota has also been described to be modulated by mushroom bioactive molecules, with implications in reducing liver inflammation during NAFLD progression.
  • non-alcoholic steatohepatitis (NASH)
    • katherine-medina
       
      I like the abbreviation
  • NASH is currently the third most common indication for liver transplantation in the United States and accounts for 10% of all HCC cases in Europe
  • ...22 more annotations...
  • The increase in nutrient availability causes systemic metabolic alterations that lead to an increase in hepatic mitochondrial respiration as well as changes in the mitochondrial lipid membrane composition.
  • They are also rich in phenolic acids, such as hydroxybenzoic and hydroxycinnamic acid, flavonoids, tocopherols, ascorbic acid and carotenoids that are known for their antioxidant activity
    • katherine-medina
       
      They are high in polyphenols, just like I had thought, I do wonder if they have tried to single out a certain flavonoid or stilbene in order to determine whether or not it was the mechanism that caused the positive reaction.
  • Of note, indeed NAFLD patients present a “metabolic inflexibility”, that is, a reduced capacity to switch back from
    • katherine-medina
       
      I did not know that
  • The increased levels of β-oxidation seem to result in an increase in citrate within the mitochondrial matrix that can be transported to the cytosol via the citrate-malate shuttle and converted to acetyl-CoA and oxaloacetate by the enzyme ATP-citrate lyase [90,106]. Indeed, NAFLD patients present increased citrate levels in plasma
  • An alternative explanation for the deficient mitochondrial respiration might be the alterations in the mitochondria lipid composition, which are already present in steatosis.
  • As defined by mitohormesis, ROS production is physiological at low levels, acting as a crucial effector in proliferation, expression of antioxidant enzymes and insulin signalling. However, high levels of ROS formation causes oxidative stress and cell damage by reacting with its different components [90,114,115]. Oxidative stress occurs when the antioxidant capacity of the cell is not sufficient to neutralize the overproduction of ROS. ROS generation causes the peroxidation of phospholipids and cardiolipin at the mitochondrial membrane
  • All these mechanisms seem to be involved in the progression from NAFL to NASH. Indeed, NASH patients present increased ROS production, DNA damage, as measured by 8-Oxo-2’-deoxyguanosine (8OHdG) levels, and hepatic lipid peroxidation coupled with decreased expression of ETC Com
    • katherine-medina
       
      So essentially NASH patients have a higher level of ROS which damages their DNA. increase of ROS = DNA damage
  • In parallel, the negative regulation on the inhibitor of apoptosis proteins (IAPs) mediated by the translocation of a series of IAP antagonists such as Smac, HTRA2/Omi and apoptosis-related protein in the TGF-ß signalling pathway (ARTS) to the cytosol, results in the release and activation of caspases
  • Aiming at weight loss, calorie-restricted diets and regular physical activity can improve hepatic mitochondria dysfunction by decreasing FFA liver input and alleviating oxidative stress.
  • New therapies need to be developed to target NAFLD and NASH,
    • katherine-medina
       
      Are there any new therapies to treat NASH and NAFLD
  • showed lipid metabolism-modulating properties in the liver
  • This may lead to a decrease in lipogenesis and a concomitant increase in β-oxidation that could explain the reduction in IHTG content [203]. Similarly, the supplementation with a 1% aqueous extract of A. cinnamomea for 8 weeks reduced the expression of leptin and increased the expression of adiponectin, which was accompanied by an increase of AMPK and PGC-1α and a reduced expression of ACC, FAS and SREBP
  • IHTG content that was similar to the positive control group, treated with rosiglitazone, a PPAR-agonist antidiabetic drug
    • katherine-medina
       
      Interesting, I wonder if there is more that can be done with this extract with these types of effects.
  • herefore, these studies suggest a pivotal capacity of mushroom extracts to counteract the detrimental oxidative damage of mitochondria in NAFLD.
  • which seems to exacerbate NASH. H2O2 over-production may open the mPTP, while its transmembrane diffusion to the cytoplasm may even result in highly detrimental OH• formation. [93,131,132]. In contrast, the capacity of mushroom extracts from species such as Pleurotus ostreatus (Jacq.) P. Kumm. (oyster mushroom) or G. lucidum to elevate the entire antioxidant defence system of hepatocytes, seems a more promising therapeutic effect against the oxidative stress in NASH.
  • . Such evidence further supports the potential of G. lucidum extracts in reversing mitochondrial dysfunction in NAFLD.
  • In this line of research, novel therapies aim to target apoptosis via mitochondria, using molecules that mimic BH3 proteins and disrupt the interactions of pro-apoptotic and anti-apoptotic proteins.
  • Both aqueous and ethanol extracts, or isolated compounds (GL22 from Ganoderma leucocontextum T.H Li, W.Q. Deng, Dong M. Wang & H.P. Hu) increased the pro-apoptotic Bax to anti-apoptotic Bcl-2/Bcl-xL ratio
  • The antitumorigenic effects of mushroom extracts and isolated compounds have also been demonstrated in in-vivo xenograft models, resulting in tumour size reduction and increased animal survival rates (Table 2). Furthermore, in the HCC Huh7 xenograft mice model, fatty acid binding proteins
  • Therefore, the mechanisms by which mushroom extracts or isolated compounds induce mitochondrial-related apoptosis pathways are diverse and may be related with specific bioactive compounds. Modulation of pathways crucial for cell survival and alterations in lipid homeostasis seem to be related with the pro-apoptotic effects observed in HCC cell lines and in in-vivo xenograft models.
    • katherine-medina
       
      Cool
  • To sum up, mitochondria play a central role in the pathophysiology and progression of NAFLD as well as in the development of HCC, which can be a late-stage consequence of NASH. Hepatic mitochondria undergo bioenergetic remodelling to face the metabolic burden imposed by the increased FFAs load secondary to systemic IR. In turn, a decompensation of these processes may result in ROS formation and mitochondrial dysfunction, contributing to the development of NASH. Lastly, hepatic mitochondria also seem to be involved in anti-apoptotic oncogenic processes driving HCC. Targeting mitochondrial dysfunction is thus a promising approach for the treatment of the NAFLD continuum. The following section describes some of the in-vitro and in-vivo studies on the beneficial effects of mushroom-enriched diets or mushroom-derived compounds/extracts (Box 2) in preventing/reverting such liver damage.
  • This distinct property of mushroom-based therapy or -containing diet is especially relevant in the multifactorial context of NAFLD and especially NASH, where systemic synergistic metabolic alterations need to be addressed.
  •  
    An article detailing a bit of the effects that mushrooms can have on the liver's mitochondrial cells.
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