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katherine-medina

Cerebrospinal Fluid (CSF) Exchange with Artificial CSF Enriched with Mesenchymal Stem C... - 2 views

www.ncbi.nlm.nih.gov/...PMC6480705

CSF-experiments mice stem-cells potential research idea HSR-2023

shared by katherine-medina on 29 May 23 - No Cached
  • Moreover, toxicity of the CSF from patients affected by neurodegenerative diseases has been reported
  • Moreover, toxicity of the CSF from patients affected by neurodegenerative diseases has been reported
    • katherine-medina
      katherine-medina on 29 May 23
       
      Good to note.
  • Moreover, no studies using biologically enriched-aCSF for exchanging the CSF have been published.
    • katherine-medina
      katherine-medina on 29 May 23
       
      That makes me want to search hard for studies that do involve the transfer, however I would have to figure out a way to create an experiment around this base idea of artificial CSF.
  • ...21 more annotations...
  • n humans, it was reported that filtration of the CSF was beneficial in Guillain-Barré syndrome [
    • katherine-medina
      katherine-medina on 29 May 23
       
      interesting that filtering the liquid can help people.
  • . MSCs produce a variety of neurogenic, neuroprotective, and immunomodulatory agents [15,16,17,18,19,20,21], and have been shown to induce beneficial effects when transplanted in EAE-mice [22,23,24,25], stroke [26,27], traumatic brain injury [28], Parkinson’s disease [29], schizophrenia, and autism [30,31] as well as increased neurogenesis in adult mice
  • A beneficial effect was also demonstrated under Aβ neurotoxicity in PC12 cells
  • Artifical cerebrospinal fluid (aCSF) enriched with secretions of mesenchymal stem cells (MSCs) increases cell viability of PC12 and SH-SY5Y neuronal cell lines
  • While secretions of 2 days growing 10 or 100 K/mL MSCs in aCSF did not show an increase in PC12 cell viability
    • katherine-medina
      katherine-medina on 29 May 23
       
      HMMM.... that is utterly fascinating the fact that after 2 days there seemed to be no change amongst the cell viability for both the 10 and 100 ml aCSF.
  • etions of 5 days growing MSCs in aCSF did show a significant increase in the PC12 cell viability relative to unenriched-aCSF treated cells
  • The principle of CSF exchange is similar to plasma exchange by plasmapheresis, which is in use for the treatment of autoimmune disorders
    • katherine-medina
      katherine-medina on 29 May 23
       
      Did not know that it was similar to plasmapheresis.
  • while cell viability was reduced under Aβ, a significant increase in cell viability was noted in the enriched-aCSF treated cells
  • A similar trend of increased cell viability by enriched-aCSF treatment was noticed in SH-SY5Y cells exposed to H2O2, but without reaching a statistical significance
  • a significant increase in cell viability was noticed in the enriched-aCSF (
  • significantly suppressed in spleen lymphocytes treated with the enriched-aCSF compared to lymphocytes treated with (unenriched-) aCSF
    • katherine-medina
      katherine-medina on 29 May 23
       
      This limiting of the lymphocytes is most likely a good thing under the guise of this paper because the suppression of lymphocytes likely helps with certain autoimmune disorders.
  • These results show that the “in/out” enriched-aCSF therapy was the most effective one, affecting both time of onset (EAE-control mice develop the disease at day 10 while the treated mice at day 14 post induction) and disease progression
  • Prolonged amelioration of EAE clinical symptoms during prolonged CSF exchange therapy: (in/out) enriched-aCSF protocol was more effective than (in) enriched-aCSF and (in/out) aCSF.
  • A trend of less demyelination in the LFB staining in the (in/out) enriched-aCSF treated- mice relative to the EAE-control mic
  • Our results show that elimination of endogenous CSF, and its replacement with MSC secretions enriched-aCSF ((in/out)-enriched-aCSF), delayed EAE onset and reduced the clinical score with indications of reduced axonal damage and demyelination.
  • in vitro and in vivo, has shown that MSCs can promote survival and axonal myelination in sensory dorsal root ganglia neurons and may be effective in non-inflammatory models of demyelination [
  • MSCs transplantation alone has been applied and tested with strong indications of beneficial effects in animal models of MS [22,23,24,25], stroke [26,27], traumatic brain injury [28], PD [29], schizophrenia, and autism
  • showing that local (intraventricular) transplantation of MSCs is more effective than intravenous administration
  • The feasibility of CSF exchange therapy reported here in the EAE model might possibly be applied to other neurodegenerative diseases of the CNS.
  • our approach of CSF exchange therapy could be beneficial for fully developed neurological diseases through a repeated application of the proposed CSF exchange protocol
    • katherine-medina
      katherine-medina on 29 May 23
       
      A good thing to recognize and note for future reference.
  • 5. Conclusions
  • katherine-medina on 29 May 23
     
    This is a more recent study about the effects of artificial CSF infused with MSC in mice.
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