Group items tagged

LC-MS/MS overcomes the limitations of immunoassays in hormone testing.  LC-MS/MS is the new gold standard with sensitivity, specificity, and ease second to none.  LC-MS/MS is the bench science now coming to the practice of medicine.

LC-MS/MS shown to be useful in evaluation in people with suspected elevated cortisol levels.  In this study, they found the dexamethasone suppression test was required.  The point however is that cortisol is a valid medium and this was with LC-MS/MS instead of the more commonly used ELIZA technique.

Great review on the literature of LC-MS/MS and now the new ESI-MS/MS method in saliva hormone testing. This technique is highly sensitive and specific. This article goes through the literature support for each hormone. It also discusses how/why saliva testing is valid. Conclusion: "the author believes that the saliva-based hormone test will be integrated into clinical practice in the near future".

LC-MS/MS shown to be "accurate and highly sensitive" when compared to GC-MS for saliva testing of hormones.  Up to this point, GC-MS has been considered the "gold standard".

salivary testosterone and DHEA using LC-MS/MS accuracy exceeds 98.5%.  Compare that with historical 50% resolution of pain with a hysterectomy.  There is no perfect test, but if accuracy exceeds 98%, you have  reliable test.

LC-MS for salivary testosterone and DHEA validated.  Also, T and DHEA were shown to have specific diurnal rhythms.  Changes in levels were also followed after DHEA therapy.

Endocrine society clinical  practice guideline's state proves validity of salivary cortisol testing.  Statement: "the diagnostic sensitivity and specificity is said to be excellent (92-100%)" and " Measurement of salivary cortisol by ELISA and LC-MS/MS has been well validated in the United States"

LC-MS/MS shows excellent sensitivity and specificity for cortisol evaluation

salivary testosterone and DHEA evaluation by LC-MS/MS validated and shown to be applicable in the diagnosis and therapy in those men with low testosterone and DHEA.

LC-MS/MS shown to be reliable in salivary cortisol evaluation.  Obese individuals create special challenges with regards to coritsol levels, due to peripheral cortisol to cortisone conversion.   This study points out that saliva should not be used solely in the diagnosis of Cushing's syndrome due to unreferenced norms.   That however, doesn't nullify salivary cortisol.  It requires a look at the references.  for those that are obese and being evaluated for cushing's.  There is no perfect test.  T This study is a little misleading in its conclusion.  Salivary testing is valid, but the reference ranges in the obese with suspected cushing's syndrome needs further evaluation: according to these authors.

LC-MS/MS will be the new gold standard in hormone evaluation.

salivary testosterone, as measured by LC-MS and ELISA, showed strong correlation with serum free "calculated" testosterone.

LC-ESI-MS/MS) for hormone evaluation has been validated for hormone testing.  Progesterone was used in this study.

Opinion publication recommends total Testosterone as primary means of evaluating Testosterone.  This recommendation relies on the use of immunoassay techniques which can lack specificity compared to newer LC/MS and GC/MS techniques.  The authors did recommend using salivary Testosterone when borderline and SHBG variables play in, which happens to be most clients I see.  The best approach is to use both simultaneously to give a more clear physiologic picture to start.

MS-LS show to be highly sensitive in the dual identification of testosterone and cortisol in saliva. These hormones were found to be stable up to 28 days at room temperature. This disproves the argument that saliva, as a test medium, has a very short shelf life.

night-time cortisol via LC shown to reliable in detection of Cushing Syndrome

specificity of saliva/salivary cortisol testing for Cushing's syndrome is 100%; but sensitivity was 69%.  Testing not done via LC-MS which is the gold standard.  This could explain the low sensitivity as compared to other studies.

salivary testosterone and androstenedione via mass spectroscopy shown to be valid.