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Casey Finnerty

ScienceDirect.com - Advances in Virus Research - Chapter 3 - Sputnik, a Virophage Infec... - 0 views

www.sciencedirect.com/...B9780123946218000133

virology mimivirus virophage

shared by Casey Finnerty on 13 Sep 12 - No Cached
  • Casey Finnerty on 13 Sep 12
     
    Did you know that there are viruses of viruses? They're called virophages, and they infect, you guessed it, mimiviruses.
Casey Finnerty

Panel Recommends HPV Vaccine for Boys and Young Men - NYTimes.com - 0 views

www.nytimes.com/...26vaccine.html

microbiology HPV vaccination virology

shared by Casey Finnerty on 29 Aug 12 - No Cached
  • HPV infection is the most common sexually transmitted disease — between 75 percent and 80 percent of females and males in the United States will be infected at some point in their lives. Most will overcome the infection with no ill effects. But in some people, infections lead to cellular changes that cause warts or cancer, including cervical, vaginal and vulvar cancers in women and anal cancers in men and women. A growing body of evidence suggests that HPV also causes throat cancers in men and women as a result of oral sex. HPV infections cause about 15,000 cancers in women and 7,000 cancers in men each year. And while cervical cancer rates have plunged over the past four decades because of widespread screening, anal cancer rates in men and women have been increasing. Head and neck cancers have also been increasing, with the share associated with HPV infection increasing rapidly — perhaps because oral sex has increased in popularity.
kchenvert09

Enterovirus and Diabetes type 1 - 0 views

www.ncbi.nlm.nih.gov/...PMC3033438

virology

shared by kchenvert09 on 26 Sep 12 - No Cached
  • kchenvert09 on 26 Sep 12
     
    This paper deals with the possibility that a virus may be a cause for diabetes type 1
jiyoung yoon

Rapid amplification of 5' complementary DNA ends... [Nat Methods. 2005] - PubMed - NCBI - 0 views

www.ncbi.nlm.nih.gov/...16145794

shared by jiyoung yoon on 27 Sep 12 - No Cached
  • jiyoung yoon on 27 Sep 12
     
    i want to know about More detailed work of 5'-RACE . from that research, i could know that 5'-race make a complementary connection with second primer required for PCR mRNA so that provides a primer-binding site upstream of the unknown 5' sequence of the target mRNA !
Sarah Muncy

ScienceDirect.com - Vaccine - Intranasal and intramuscular immunization with Baculoviru... - 0 views

www.sciencedirect.com/...S0264410X10009412

shared by Sarah Muncy on 27 Sep 12 - No Cached
  • An anti-malarial transmission-blocking vaccine (TBV) that prevents fertilization and/or ookinete/oocyst development within the mosquito is an attractive strategy to limit the transmission of malaria
  • The present study used this system to generate a Plasmodium vivax transmission-blocking immunogen (AcNPV-Dual-Pvs25).
  • Plasmodium vivax
  • ...10 more annotations...
  • A variety of expression vectors (e.g., Escherichia coli, Pichia pastoris and DNA) have been used to express Pvs25 protein which has been administered alone or in combination with adjuvants
  • To date these studies suggest that the recombinant protein currently requires both not only linear, but conformation dependent epitopes, and a strong adjuvant to induce transmission-blocking antibodies.
  • Intranasal and intramuscular immunization with Baculovirus Dual Expression System-based Pvs25 vaccine substantially blocks Plasmodium vivax transmission
  • Recently, we have developed a new vaccine vector system based on the baculovirus Autographa californica nucleopolyhedrosis virus (AcNPV) termed the “Baculovirus Dual Expression System”, which drives expression of vaccine candidate antigens by a dual promoter that consists of tandemly arranged baculovirus-derived polyhedrin and mammalian-derived CMV promoters. It has been shown that AcNPV, an enveloped double-stranded DNA virus that naturally infects insects, possesses strong adjuvant properties that can activate dendritic cell-mediated innate immunity
  • Mucosal vaccines have several attractive features compared with parenteral vaccines (e.g., safety, cost-effectiveness and ease of administration), but studies on their use have been limited almost exclusively to protection against mucosally transmitted pathogens. We provide evidence that i.n. immunization is a feasible alternative for preventing malaria, which is transmitted through non-mucosal routes
  • These results are consistent with our previous work showing that intranasal immunization with the baculovirus-based vaccine induced strong systemic humoral immune responses with high titres of antigen-specific antibodies and conferred complete protection against malaria blood-stage challenge
  • which can induce immunological memory against heterologous antigens in a rodent model; however, it is precluded from clinical use due to its enterotoxicity and potential hazardous effects on olfactory nerves [22]. In contrast, a baculovirus-based delivery system may offer an attractive immunization method, as AcNPV exhibits low cytotoxicity and is incapable of replication in mammalian cells
  • The data described here adds to previously presented data showing the significant potential of the baculovirus dual expression system against the blood stages of the parasite
  • but also demonstrates clearly its ability to induce antibodies against the ookinete surface protein Pvs25, and to elicit a transmission-blocking immune response against the P. vivax isolates from endemic areas, and a transgenic rodent malaria parasite model in preliminary studies.
  • One was SMFA on peripheral blood from P. vivax infected patients.
  • Sarah Muncy on 27 Sep 12
     
    Reference paper #2. Gave me information on malaria and baculoviruses.
mattgreatens

French Experience of 2009 A/H1N1v Influenza in Pregnant Women - 0 views

www.plosone.org/...10.1371%2Fjournal.pone.0013112

H1N1 Avian Influenza

shared by mattgreatens on 02 Oct 12 - No Cached
  • mattgreatens on 02 Oct 12
     
    This is another supplementary article for my paper.
sdahlseng10

Possible applications for replicating HIV 1 vectors - 0 views

www.ncbi.nlm.nih.gov/...PMC2889699

vaccine HIV vector gene therapy virotherapy

shared by sdahlseng10 on 02 Oct 12 - No Cached
  • sdahlseng10 on 02 Oct 12
     
    Review Article; Journal club 1: This is a basic review article about HIV virus vectors and their potential uses in clinical science.
sdahlseng10

Improved lentiviral vectors for Wiskott-Aldrich syndrome gene therapy mimic... - 0 views

web.ebscohost.com.wsuproxy.mnpals.net/...pdfviewer

gene therapy hematopoietic cells immunodeficiency HIV

shared by sdahlseng10 on 02 Oct 12 - No Cached
  • sdahlseng10 on 02 Oct 12
     
    Supplemental Article to Lesch et al. (2011): This article talks about one of the disorders that is treatable by gene therapy: a relatively rare x-linked chromosomal abnormality that causes immunodeficiency as a result of hematopoietic cell damage causing inefficient T-cell receptor binding. Lentiviruses appear to be good vectors for the gene therapy treatment of this disorder as a highly regulated therapy is required for a beneficial outcome.
Casey Finnerty

Functional importance of deletion mut... [Appl Environ Microbiol. 2005] - PubMed - NCBI - 0 views

www.ncbi.nlm.nih.gov/...16085811

virology baculoviruses genomics population biology genetics

shared by Casey Finnerty on 25 Sep 12 - No Cached
  • Casey Finnerty on 25 Sep 12
     
    This paper shows the importance of genotypic diversity for baculovirus populations, particularly in terms of pathogenicity.
sdahlseng10

Replication-competent Lentivirus Analysis of Clinical Grade Vector Products - 0 views

www.ncbi.nlm.nih.gov/...PMC3048188

HIV lentivirus vector replication competent gene therapy

shared by sdahlseng10 on 02 Oct 12 - No Cached
  • sdahlseng10 on 02 Oct 12
     
    Supplementary Article to Lesch et al. (2011); journal club 1: This article helps us understand the dangers of attenuated lentiviruses used for gene therapy and other clinical applications. It also details a method for screening newly synthesized lentiviruses for replication competency.
sdahlseng10

In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector - 0 views

www.jstor.org.wsuproxy.mnpals.net/...2889637.pdf

gene therapy nondividing cells lentivirus vector

shared by sdahlseng10 on 02 Oct 12 - No Cached
  • sdahlseng10 on 02 Oct 12
     
    Supplemental Article to Lesch et al. (2011); Journal club 1: This article provides interesting support to the effectiveness of lentivirus vectors for gene therapy treatment. Not only is HIV or lentivirus efficient at transduction to rapidly dividing cells, it is also capable of transduction to nondividing cells; making it an exciting vector for gene therapy treatment. The range of diseases that could be treatable with a gene therapy vector that can target other cell types than hematopoietic cells could transform the science of gene therapy.
mattgreatens

Pneumonia and Respiratory Failure from Swine-Origin Influenza A (H1N1) in Mexico - 0 views

www.nejm.org/...NEJMoa0904252

H1N1 Avian Influenza

shared by mattgreatens on 02 Oct 12 - No Cached
  • mattgreatens on 02 Oct 12
     
    This is one of my supplementary articles.
mattgreatens

Spreading Patterns of the Influenza A (H1N1) Pandemic - 0 views

www.plosone.org/...10.1371%2Fjournal.pone.0017823

H1N1 Avian Influenza

shared by mattgreatens on 02 Oct 12 - No Cached
  • mattgreatens on 02 Oct 12
     
    This is the final supplementary article.
Casey Finnerty

For Young Scientists, A Wild Ride - 0 views

www.sciencemag.org/...1495.full

virology microbiology Influenza

shared by Casey Finnerty on 02 Oct 12 - No Cached
  • Casey Finnerty on 02 Oct 12
     
    This article discusses the drama behind the scenes of the debate over publication of the H5N1 transmissibility studies and the effect it had on young scientists carrying out the work.
Sarah Muncy

ScienceDirect.com - Journal of Bioscience and Bioengineering - Efficient production of ... - 0 views

www.sciencedirect.com/...S138917231000191X

shared by Sarah Muncy on 26 Sep 12 - No Cached
  • Sarah Muncy on 26 Sep 12
     
    This article about baculoviruses goes into how not only can baculoviruses make simple proteins, or express angitens of forgein substances, they can also make pieces of antibodies!
Sarah Muncy

ScienceDirect.com - Vaccine - Hemagglutinin Displayed Baculovirus Protects Against High... - 0 views

www.sciencedirect.com/...S0264410X10011850

shared by Sarah Muncy on 26 Sep 12 - No Cached
    • Sarah Muncy
      Sarah Muncy on 26 Sep 12
       
      So, the baculovirus on TOP of having the H5HA on it, can also get the immune system to kick in better?
  • It is remarkable that low doses (103pfu/mouse) of BVs act as an effective adjuvant [41]. Therefore, reducing BV concentration and elongating vaccination intervals may prevent memory responses to BV administration
  • scanning densitometry
  • ...8 more annotations...
  • Foreign immunogens or peptides can be displayed on the envelope of AcMNPV by fusion with the baculovirus major envelope protein gp64
  • Baculoviruses have strong adjuvant activity to promote humoral and cellular immune responses against coadministered antigens, activate dendritic cells maturation, induce the production of cytokines, chemokines, and type I IFNs
  • There are two influenza vaccine approaches licensed in the US; the inactivated, split vaccine and the live-attenuated virus vaccine. Inactivated vaccines can efficiently induce humoral immune responses but generally only poor cellular immune responses.
  • Therefore, influenza HA can be displayed on the surface of baculovirus
  • virus-like particle (VLP)
  • Even though cellular immune responses cannot confer sterilizing immunity, they are able to reduce the severity of infection and lower morbidity and mortality rates [47], and antigen-specific memory T cells are able to rapidly respond to a secondary virus infection [45]. Furthermore, cellular immune responses to the conserved epitopes contained in vaccines may provide cross-protective immunity against different subtypes of influenza virus infection
  • To confirm that each HA was incorporated on the envelope of baculoviruses, supernatants from infected Sf9 cells were used to perform hemagglutination assay
  • Most BV display strategies rely on gp64 protein which is the major envelope protein of baculovirus.
  • Sarah Muncy on 26 Sep 12
     
    This paper gave me a better understanding of some aspects of my focal paper that were unclear. How to test for HA, and how baculoviruses may be adjuvants in addition to expression vectors.
Casey Finnerty

Judy Mikovits in Prison: What Does It Mean for Research on Chronic Fatigue Syndrome? - ... - 0 views

www.slate.com/...chronic_fatigue_syndrome_.html

research microbiology virology

shared by Casey Finnerty on 29 Aug 12 - No Cached
  • This created a climate of mistrust that breeds hero-worship and conspiracy theories and that can cast a scientist simultaneously as a savior and a villain.
Sarah Muncy

PLOS ONE: Safety and Immunogenicity of H5N1 Influenza Vaccine Based on Baculovirus Surf... - 0 views

www.plosone.org/...5E58993766A3A1256696729D429B79

shared by Sarah Muncy on 26 Sep 12 - No Cached
  • Sarah Muncy on 26 Sep 12
     
    This paper gives a descriptive about how they were able to make a candidate vaccine for influenza that is really cheap/safe and very effective using Bombyx mori caterpillars as bioreactors to get needed proteins.
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