Taken together, these data indicate that ascorbate at concentrations achieved
only by i.v. administration may be a pro-drug for formation of H2O2, and that blood can be a delivery system of the pro-drug to tissues.
Group items matching
in title, tags, annotations or url
1More
Karma 8020 Wheelchair - 0 views
www.wheelchairindia.com/...a-8020-X-Heavy-Duty-Wheelchair
Extra Wide Heavy Duty Wheelchair reinforced cross braces Wheelchair Heavy Duty strong structure design larger turning radius Karma 8020 Wheelchair
shared by wheelchairindia9 on 18 Apr 15
- No Cached
-
wheelchairindia9 on 18 Apr 15This wheelchair of choice for those who desire an extra wide, heavy-duty chair. Bariatric equipment is designed to be used in the care of large and heavy individuals. With childhood obesity levels also rising, the needs of heavier patients and those caring for them, have to be addressed. Wheelchairs that have been constructed for use by the heaviest individuals have built-in strengthening and bracing of the frame, to reduce the possibility of it twisting or buckling in use. The bariatric chairs are available in 20 and 22 inch widths with a red or blue frame. It comes with dual, reinforced steel cross braces for heavy duty support. The seat upholstery is reinforced for long life and comes with a back carry pouch for small items.The 12″ rear wheels are flat free, and the desk length arms are removable and reversible. Features bariatric chairs: Dual, reinforced steel cross braces. Supports individuals. Reinforced steel frame provides added support. Heavy duty, nylon reinforced upholstery with a back carry pouch. Removable, reversible desk length arms. Adjustable leg support. 12″ flat free rear wheels. Using a Karma KM-8020: wheelchairs require more strength to push by the caregiver and by the user, if the chair is self-propelled. In some cases, it may be wise for the caregiver to ask for assistance from another person in order to push the wheelchair safely. Because heavy duty wheelchairs are wider than standard wheelchairs, they require wider doorways and a larger turning radius. They are also heavier to lift into a vehicle and harder to push uphill. Features Can be used as standard self propelled wheelchair or a transport chair all in one. Quick release 24″ wheels can be removed to transition self propelled chair to a transport chair. All aluminum frame. Comes with two sets of aluminum wheel locks: one for use with wheelchair, one for use with transport chair. Fold down back with deluxe back release. Comes standard with swingaway footre
1More
Tricycle For Disabled Children - 0 views
www.wheelchairindia.com/...Handicapped-Child-Tricycle
Tricycle For Adults And Children With Special Needs shape of a conventional bicycle leg calibers various seating and bar adjustment reduced power height adjustable backrests control poles steering restrictors
shared by wheelchairindia9 on 17 Apr 15
- No Cached
-
The tricycles are individually designed to meet the needs of each disabled child and help provide physiotherapy, build muscle tone and promote play. Italso help benefit growth and development in children with disabilities, enabling them to keep active and retain their independence. Disabled children get huge physical and emotional benefits from having the specially tricycles, which are each custom made to suit the individual child's needs. Riding a tricycle provides both fun and fitness, and is a therapeutic activity that allows children to exercise their lower extremities. As the muscles move through cycling motions, they are flexed, extended and stretched. This range of motion is crucial for children with disabilities, because muscles are incapable of keeping up with bone growth unless fully extended. Tricycles are provided to severely disabled children and adults and come in two sizes, 20 inch and 24 inch models. These trikes are grant funded and to qualify for one of these, an individual must suffer from a condition such as, but not limited to, Cerebral Palsy, Down Syndrome, Traumatic Brain Injury or Autism. Trunk support system is ideal for riders who have trouble remaining upright while seated. An optional headrest is available. A headrest can be purchased with either a flat or contoured headpiece, and adjusts both horizontally and vertically for optimum positioning. The Front Pulley System maintains a level pedal position for children whose extreme tone forces the front of the pedal downward. Turn the oval knob to raise and lower the handlebar. A handbrake for the conventional handlebar will arrive installed on Large tricycle. Open access combined with a low transfer step makes it easy to get on and off trikes. Tricycle Deluxe Double Hand Drive: Frame : Made by E.R.W. Tubes 38.0 mm-16 G. 25.4 mm & 22.22 mm- 18 G. Seat & Back : M S, C.R.C. Sheet. Seat Size 18"x 16" Wheel Size : Wheel Diameter 24"x 1 1/2" Tyre and Tube Standard Company. Pa
1More
Electronic Wheel chair - 0 views
www.wheelchairindia.com/...POWER-WHEELCHAIR
Shower Wheelchair Can Dramatically Improve Lives Electronic Wheel chair electric propulsion by motors a joy stick or push buttons shower wheelchair lightweight and portable for travel accessible seat frame
shared by wheelchairindia9 on 16 Apr 15
- No Cached
-
Golden Technologies takes all the worry out of purchasing a power wheelchair. Designers blend the most technologically advanced components with superior designs to provide with the almost in quality, comfort and style. Motorized wheelchairs to enter bike lanes if a sidewalk isn't available or passable by wheelchair. Wheelchair users would be required to yield the right-of-way to bikers. The proposal also adds motorized wheelchairs to current law affecting vehicles and bicycles on the road, including the required three foot law between bikers and drivers. Supporters bill would help wheelchair users get around quickly and that areas without sidewalks are often difficult to navigate. It's the standard chair, with the main frame attaches to the front. This isn't a futuristic design as such, but a very good way of maintaining or building strength in those who are bound to a wheelchair, and may hope to walk again in their future. Personally, this bike is pretty cool. It's got a front frame similar to a chopper, with the added comfort of a chair appose to a saddle. A new module is set to transform electric powered wheelchairs into communication hubs. An powerchair is more compact and has a better turning radius than an electric scooter; making it is easier to navigate narrow doorways and tight turns. Another advantage of the powerwheelchair is that its armchair joystick does not require an upright posture like an electric scooter's handlebars. Most power wheelchairs can also be taken apart and stowed, while scooters usually can't. Powerwheelchairs are also usually less expensive than scooters. For many disabled people, the only way to move around is by using a wheelchair. Those who cannot powered wheelchairs propel themselves with their arms, which often leads to fatigue, pain, and even permanent damage to arms and shoulders. BENEFITS: Activates circulation system and improves cardio-pulmonary function. Helps prevent decubitus sores. Improves bowel regu
57More
Pharmacologic ascorbic acid concentrations selectively kill cancer cells: Action as a p... - 0 views
www.pnas.org/...13604.full
IV vitamin C cancer ascorbic acid vitamin C H2O2 GPX glutathione Perioxidase
shared by Nathan Goodyear on 09 Feb 11
- Cached
-
These findings give plausibility to i.v. ascorbic acid in cancer treatment, and have unexpected implications for treatment of infections where H2O2 may be beneficial
-
pharmacologic concentrations of ascorbate killed cancer but not normal cells, that cell death was dependent only on extracellular but not intracellular ascorbate, and that killing was dependent on extracellular hydrogen peroxide (H2O2) formation with ascorbate radical as an intermediate
- ...48 more annotations...
-
Our data show that ascorbic acid selectively killed cancer but not normal cells, using concentrations that could only be achieved by i.v. administration
-
Ascorbate-mediated cell death was due to protein-dependent extracellular H2O2 generation, via ascorbate radical formation from ascorbate as the electron donor. Like glucose, when ascorbate is infused i.v., the resulting pharmacologic concentrations should distribute rapidly in the extracellular water space (42). We showed that such pharmacologic ascorbate concentrations in media, as a surrogate for extracellular fluid, generated ascorbate radical and H2O2. In contrast, the same pharmacologic ascorbate concentrations in whole blood generated little detectable ascorbate radical and no detectable H2O2. These findings can be accounted for by efficient and redundant H2O2 catabolic pathways in whole blood (e.g., catalase and glutathione peroxidase) relative to those in media or extracellular fluid
-
ascorbic acid administered i.v. in pharmacologic concentrations may serve as a pro-drug for H2O2 delivery to the extracellular milieu
-
H2O2 generated in blood is normally removed by catalase and glutathione peroxidase within red blood cells, with internal glutathione providing reducing equivalents
-
The electron source for glutathione is NADPH from the pentose shunt, via glucose-6-phosphate dehydrogenase. If activity of this enzyme is diminished, the predicted outcome is impaired H2O2 removal causing intravascular hemolysis, the observed clinical finding.
-
Only recently has it been understood that the discordant clinical findings can be explained by previously unrecognized fundamental pharmacokinetics properties of ascorbate
-
Intracellular transport of ascorbate is tightly controlled in relation to extracellular concentration
-
Intravenous ascorbate infusion is expected to drastically change extracellular but not intracellular concentrations
-
For i.v. ascorbate to be clinically useful in killing cancer cells, pharmacologic but not physiologic extracellular concentrations should be effective, independent of intracellular ascorbate concentrations.
-
There was no correlation with ascorbate-induced cell death and glutathione, catalase activity, or glutathione peroxidase activity.
-
H2O2, as the product of pharmacologic ascorbate concentrations, has potential therapeutic uses in addition to cancer treatment, especially in infections
-
Use of ascorbate as an H2O2-delivery system against sensitive pathogens, viral or bacterial, has substantial clinical implications that deserve rapid exploration.
-
Recent pharmacokinetics studies in men and women show that 10 g of ascorbate given i.v. is expected to produce plasma concentrations of nearly 6 mM, which are >25-fold higher than those concentrations from the same oral dose
-
As much as a 70-fold difference in plasma concentrations is expected between oral and i.v. administration,
-
Complementary and alternative medicine practitioners worldwide currently use ascorbate i.v. in some patients, in part because there is no apparent harm
-
We first investigated whether ascorbate in pharmacologic concentrations selectively affected the survival of cancer cells by studying nine cancer cell lines
-
Clinical pharmacokinetics analyses show that pharmacologic concentrations of plasma ascorbate, from 0.3 to 15 mM, are achievable only from i.v. administration
-
plasma ascorbate concentrations from maximum possible oral doses cannot exceed 0.22 mM because of limited intestinal absorption
-
For five of the nine cancer cell lines, ascorbate concentrations causing a 50% decrease in cell survival (EC50 values) were less than 5 mM, a concentration easily achievable from i.v. infusion
-
All tested normal cells were insensitive to 20 mM ascorbate.
-
As ascorbate concentration increased, the pattern of death changed from apoptosis to pyknosis/necrosis, a pattern suggestive of H2O2-mediated cell death
-
Apoptosis occurred by 6 h after exposure, and cell death by pyknosis was ≈90% at 14 h after exposure
-
In contrast to lymphoma cells, there was little or no killing of normal lymphocytes and monocytes by ascorbate
-
Ascorbate is transported into cells as such by sodium-dependent transporters, whereas dehydroascorbic acid is transported into cells by glucose transporters and then immediately reduced internally to ascorbate
-
Whether or not intracellular ascorbate was preloaded, extracellular ascorbate induced the same amount and type of death.
-
extracellular ascorbate in pharmacologic concentrations mediates death of lymphoma cells by apoptosis and pyknosis/necrosis, independently of intracellular ascorbate.
-
Because these data implicated H2O2 in cell killing, we added H2O2 to lymphoma cells and studied death patterns using nuclear staining (19, 28). The death patterns found with exogenous H2O2 exposure were similar to those found with ascorbate
-
For both ascorbate and H2O2, death changed from apoptosis to pyknosis/necrosis as concentrations increased
-
Sensitivity to direct exposure to H2O2 was greater in lymphoma cells compared with normal lymphocytes and normal monocytes
-
There was no association between the EC50 for ascorbate-mediated cell death and intracellular glutathione concentrations, catalase activity, or glutathione peroxidase activity
-
H2O2 generation was dependent on time, ascorbate concentration, and the presence of trace amounts of serum in media
-
whatever H2O2 is generated should be removed by glutathione peroxidase and catalase within red blood cells, because H2O2 is membrane permeable
-
The data are consistent with the hypothesis that ascorbate in pharmacologic concentrations is a pro-drug for H2O2 generation in the extracellular milieu but not in blood.
-
In patients with glucose-6-phosphate dehydrogenase deficiency, i.v. ascorbate is contraindicated because it causes intravascular hemolysis
-
ascorbate, an electron-donor in such reactions, ironically initiates pro-oxidant chemistry and H2O2 formation
-
data here showed that ascorbate initiated H2O2 formation extracellularly, but H2O2 targets could be either intracellular or extracellular, because H2O2 is membrane permeant
-
More than 100 patients have been described, presumably without glucose-6-phosphate dehydrogenase deficiency, who received 10 g or more of i.v. ascorbate with no reported adverse effects other than tumor lysis
8More
Effects of short-term high-fat overfeeding on genome-wide DNA methylation in the skelet... - 0 views
-
The induction of DNA methylation changes after 5 days of HFO supports the growing awareness of DNA methylation as a dynamic signal that is possibly relevant to short-term day-to-day metabolic adaptations, including acute exercise
- ...4 more annotations...
-
Diverging DNA methylation levels between elderly, but not young, genetically identical twins indicate that environmental exposures throughout life may permanently influence DNA methylation, suggesting some preservation of de novo DNA methylation in adults
-
our finding of a slow reversibility rate indicates the demethylation process may be somewhat impeded compared with the induction of methylation changes by diet, which could have implications for the preservation or build-up of CpG methylation over time
-
A slow reversibility of DNA methylation induced by carcinogenic agents has likewise been observed due to ingestion of high-fat diets in rodents
Cancer Metastasis: Building a Framework: Cell - 0 views
1More
Blog - Best Wordpress Developer / Digital Marketing Agency in Atlanta, GA - 0 views
A Review of the Mechanism of Injury and Treatment Approaches for Illness Resulting from... - 0 views
6More
IgG4 Antibodies Induced by Repeated Vaccination May Generate Immune Tolerance to the SA... - 0 views
www.ncbi.nlm.nih.gov/...PMC10222767
spike protein spike proteins IgG4 COVID19 COVID-19 COVID vaccination SARS-CoV-2
shared by Nathan Goodyear on 01 Jun 23
- No Cached
-
These mRNA vaccines represented a new type of vaccine that comprises synthetic mRNA molecules that contain the coding sequence necessary to build the SARS-CoV-2 Spike protein, which is encased in the lipid nanoparticles (LNPs) to allow for the delivery of mRNA to cells.
- ...3 more annotations...
-
provide the scientific rationale suggesting that repeated vaccination with mRNA vaccines could generate an immune tolerance mechanism, thereby favoring unopposed SARS-CoV-2 replication.
13More
We're Not "DON" Yet: Optimal Dosing and Prodrug Delivery of 6-Diazo-5-oxo-L-norleucine ... - 0 views
-
Rapidly proliferating healthy cells (GI epithelium, lymphocytes) or cells under physiologic stress have increased demand for glutamine
-
Glutamine is transported into cells by one of multiple amino acid transporters (e.g. ASCT2, BOAT2), several of which are thought to be upregulated in cancer cells
- ...10 more annotations...
-
Glutamate, produced from glutamine by glutaminase and glutamine amidotransferase activities, may be further metabolized to alpha ketoglutarate and provide a carbon skeleton source for the mitochondrial tricarboxylic acid cycle (TCA cycle)
-
Glutamine-derived glutamate is also involved in the synthesis of the reducing equivalent glutathione, vital to maintaining cellular redox status
-
Many tumors become largely dependent on glutamine to provide carbon and nitrogen building blocks needed for proliferation
-
In cancer model systems, Eagle and colleagues first demonstrated tumor cells in culture require supplementation with exogenous glutamine for efficient proliferation
-
The most well-characterized oncogene to regulate glutamine metabolism is MYC (9), which enhances glutaminase expression, upregulates glutamine transporters, and enhances glutamine utilization in energy production and biosynthesis
-
Other pro-tumorigenic regulators such as KRAS and mTOR, as well as tumor suppressors (p53, VHL) have also been associated with alterations in glutamine metabolism
-
Tumor cells are highly adaptable and alter nutrient uptake and metabolic networks to resist single agent glutaminase inhibition
-
cells in the microenvironment of several tumor types upregulate glutamine production, thereby enabling tumor cells to escape glutaminase inhibition