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Digital Storytelling is the modern expression of the ancient art of storytelling. Digital stories derive their power by weaving images, music, narrative and voice together, thereby giving deep dimension and vivid color to characters, situations, experiences, and insights. Tell your story now digitally. - Leslie Rule, Digital Storytelling Association

The Center for Digital Storytelling is an international not-for-profit community arts organization rooted in the craft of personal storytelling. We assist youth and adults around the world in using media tools to share, record, and value stories from their lives, in ways that promote artistic expression, health and well being, and justice.

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MicroRNA(miRNA) is a class of endogenous non-coding RNA found in eukaryotes with regulatory functions. They are about 20~25 nt. miRNAs are evolutionally-conserved and have a rich variety of species, they are not translated into proteins, but play an important role in regulating gene expression. miRNA can specifically bind to complementary regions within messenger RNA(mRNA) to promote its degradation or inhibit translation. miRNA is widely involved in cell proliferation, differentiation, apoptosis and cell cycle regulation, and plays a key role in gene experiments.

To create a lentivirus stable cell line, cells are typically transduced with lentiviral particles containing the desired gene of interest. The lentiviral vector integrates into the host cell's genome, leading to stable expression of the gene in the cell line. This allows researchers to study the effects of the gene of interest in a controlled and reproducible manner.

Lentivirus vector is a gene therapy vector developed on the base of HIV-1 (human immunodeficiency virus type 1). Lentivirus vector is a powerful tool for introduction of exogenous genes, with the advantages of wide infection spectrum, effective infection and long-term stable expression of exogenous genes. Now the lentivirus system has been widely used in various cell lines for gene overexpression, RNA interference, microRNA research, as well as in vivo experiments.

The mycoplasma, lacking a cell wall, is the smallest prokaryotic cell. During the process of cell culture, the contamination rate of mycoplasma exceeds 60%. When cells (especially passage cells) are contaminated by mycoplasma, it alters the expression of DNA, RNA, and proteins within these cells. Severe contamination leads to slow cell growth, changes in cellular morphology, and cytomorphy development. Consequently, preventing mycoplasma contamination during cell culture remains a global challenge.

Adeno-Associated Virus (AAV package) belongs to the family of parvovirus and is a non-enveloped single-stranded DNA virus, with a genome size of 4.7 kb composed of two open reading frames (ORFs) flanked by inverted terminal repeat sequences (ITRs) of 145 nucleotides each. AAV vectors are one of the most commonly used vectors for a variety of gene therapy applications and have been widely used in clinics and basic research mainly owing to their capable of transducing a wide range of species and tissues in vivo with no evidence of toxicity, and it generates relatively mild innate and adaptive immune responses as well as their long-term transgene expression and steady physical properties.

In the biomedical field, the transfer of plasmid vectors into mammalian cells using conventional transfection is the most widely used method. Although a number of more advanced gene introduction vector systems have been developed in recent years (e.g., lentiviral vectors, adenoviral vectors, AAV vectors, and piggyBac, etc.), conventional plasmid gene expression vectors are still used in the majority of laboratories, mainly due to their technical simplicity and efficient transfection rates in various types of cells.

ptogenetics and chemical genetics are powerful tools used in neuroscience and molecular biology to study and manipulate cellular processes with high spatiotemporal precision. These tools allow researchers to control and modulate cellular activity, signaling pathways, and gene expression in a targeted and reversible manner.

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