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To create a lentivirus stable cell line, cells are typically transduced with lentiviral particles containing the desired gene of interest. The lentiviral vector integrates into the host cell's genome, leading to stable expression of the gene in the cell line. This allows researchers to study the effects of the gene of interest in a controlled and reproducible manner.

Lentivirus vector is a gene therapy vector developed on the base of HIV-1 (human immunodeficiency virus type 1). Lentivirus vector is a powerful tool for introduction of exogenous genes, with the advantages of wide infection spectrum, effective infection and long-term stable expression of exogenous genes. Now the lentivirus system has been widely used in various cell lines for gene overexpression, RNA interference, microRNA research, as well as in vivo experiments.

A pseudovirus is a laboratory-created viral particle that mimics the structure of a real virus but lacks the ability to replicate and cause infection. Pseudoviruses are formed by encapsulating specific nucleic acid fragments within a viral capsid protein. Currently, the pseudoviruses launched by Hanbio Biotechnology are mainly lentivirus, adenovirus and adeno-associated virus. The quality of products is strictly controlled to ensure the stability between batches. Pseudoviruses are mainly used as positive control standard and internal control standard in viral nucleic acid test kits.