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Nathan Goodyear

Development and validation of stable-isotope dilution liquid chromatography-tandem mass spectrometric method for determination of salivary progesterone - Higashi - 2011 - Biomedical chromatography - Wiley Online Library - 0 views

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    LC-ESI-MS/MS) for hormone evaluation has been validated for hormone testing.  Progesterone was used in this study.
Nathan Goodyear

Diagnostic significance of salivary testosterone measurement revisited: using liquid chromatography/mass spectrometry and enzyme-linked immunosorbent assay - 0 views

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    salivary testosterone, as measured by LC-MS and ELISA, showed strong correlation with serum free "calculated" testosterone.
Nathan Goodyear

Rapid determination of natural steroidal hormones in saliva for the clinical diagnoses - 0 views

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    This study looked at combined sex hormone analysis via gas chromatography-mass spec.  Levels were able to be detected as low as 0.002 mcro/L in saliva.  This study validates the GC-MS for the use of multiple, simultaneous steroid hormone analysis
Nathan Goodyear

ScienceDirect.com - Journal of Chromatography B - Simultaneous determination of salivary testosterone and dehydroepiandrosterone using LC-MS/MS: Method development and evaluation of applicability for diagnosis and medication for late-onset hypogonadism - 0 views

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    LC-MS for salivary testosterone and DHEA validated.  Also, T and DHEA were shown to have specific diurnal rhythms.  Changes in levels were also followed after DHEA therapy.
Nathan Goodyear

Evaluation of a Candidate Reference Measurement Procedure for Serum Free Testosterone Based on Ultrafiltration and Isotope Dilution-Gas Chromatography-Mass Spectrometry - 0 views

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    Though this study looked  at serum testosterone, GC-MS was shown to be valid technique to evaluate testosterone levels.
Nathan Goodyear

The role of salivary cortisol measured by liquid chromatography-tandem mass spectrometry in the diagnosis of subclinical hypercortisolism - 0 views

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    LC-MS/MS shown to be useful in evaluation in people with suspected elevated cortisol levels.  In this study, they found the dexamethasone suppression test was required.  The point however is that cortisol is a valid medium and this was with LC-MS/MS instead of the more commonly used ELIZA technique.
Nathan Goodyear

Simultaneous d... [J Chromatogr B Analyt Technol Biomed Life Sci. 2009] - PubMed - NCBI - 0 views

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    salivary testosterone and DHEA evaluation by LC-MS/MS validated and shown to be applicable in the diagnosis and therapy in those men with low testosterone and DHEA.
Nathan Goodyear

Comparison of Liquid Chromatography-Tandem Mass Spectrometry, RIA, and ELISA Methods for Measurement of Urinary Estrogens - 0 views

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    Mass Spectrometry better than RIA and ELISA for urinary estrogen metabolite testing.
Nathan Goodyear

Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) Assay for Simultaneous Measurement of Salivary Testosterone and Cortisol in Healthy Men for Utilization in the Diagnosis of Late-onset Hypogonadism in Males - 0 views

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    MS-LS show to be highly sensitive in the dual identification of testosterone and cortisol in saliva. These hormones were found to be stable up to 28 days at room temperature. This disproves the argument that saliva, as a test medium, has a very short shelf life.
Nathan Goodyear

Measurement of salivary cortisol with liquid chromatography-tandem mass spectrometry in patients undergoing dynamic endocrine testing - Perogamvros - 2009 - Clinical Endocrinology - Wiley Online Library - 1 views

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    LC-MS/MS shows excellent sensitivity and specificity for cortisol evaluation
Nathan Goodyear

Diagnostic Characteristics of Late-Night Salivary Cortisol Using Liquid Chromatography-Tandem Mass Spectrometry - 0 views

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    night-time cortisol via LC shown to reliable in detection of Cushing Syndrome
Nathan Goodyear

Salivary Hormone Measurement Using LC/MS/MS: Specific and Patient- Friendly Tool for Assessment of Endocrine Function - 0 views

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    Great review on the literature of LC-MS/MS and now the new ESI-MS/MS method in saliva hormone testing. This technique is highly sensitive and specific. This article goes through the literature support for each hormone. It also discusses how/why saliva testing is valid. Conclusion: "the author believes that the saliva-based hormone test will be integrated into clinical practice in the near future".
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