"CRACKING THE CODE"/"Cloning Paper Plasmid" activities can (1) serve as a review of the "genetic code" and the role it plays in our life; and, (2) to help students see how genes may be manipulated for genetic research, namely, gene cloning/genetic engineering.
The laboratory time, the specialized equipment and expertise to carry out recombinant DNA experiments may be lacking in the high school. Activity 2 will help students conceptualize the mechanics involved in cutting and ligating DNAs into a plasmid vector with "sticky ends" of complementary DNA base pairs.
3 min video, Scientists have made a major advancement in the world of cloning-- creating hundreds of identical mice from a single drop of blood. The implications are pretty big. Why? Anthony Carboni as the answers.
LONDON, England- (24 July 1997)- Move over Dolly, and make room for Polly, the first sheep cloned by nuclear transfer technology bearing a human gene. Investors hope the lamb will make them a mint, but more importantly, the arrival of Polly could be good news for hemophiliacs and others who rely on expensive protein therapy of their diseases.
In molecular biology, ligation refers to the joining of two DNA fragments through the formation of a phosphodiester bond. An enzyme known as a ligase catalyzes the ligation reaction. In the cell, ligases repair single and double strand breaks that occur during DNA replication. In the laboratory, DNA ligase is used during molecular cloning to join DNA fragments of inserts with vectors - carrier DNA molecules that will replicate target fragments in host organisms.
9:30 video Hank & his clone Circus Hank explain the power of epigenetics, which studies the factors that determine how much or whether some genes are expressed in your body.
In one of the first successful attempts at genetically engineering mosquitoes, HHMI researchers have altered the way the insects respond to odors, including the smell of humans and the insect repellant DEET. The research not only demonstrates that mosquitoes can be genetically altered using the latest research techniques, but paves the way to understanding why the insect is so attracted to humans, and how to block that attraction. "The time has come now to do genetics in these important disease-vector insects.
What effects will the rapidly growing field of synthetic biology have on the conservation of nature? The ecological and ethical challenges stemming from this question will require a new and continuing dialogue between members of the synthetic biology and biodiversity conservation communities, according to authors of a new paper.