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The analytical supercritical fluid chromatograph uses supercritical fluid (such as CO2) with good dissolution and mass transfer characteristics as the mobile phase, and optimizes the analysis and preparation conditions by adjusting the flow rate and composition of the mobile phase, as well as the system temperature and pressure.

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In the biomedical field, the transfer of plasmid vectors into mammalian cells using conventional transfection is the most widely used method. Although a number of more advanced gene introduction vector systems have been developed in recent years (e.g., lentiviral vectors, adenoviral vectors, AAV vectors, and piggyBac, etc.), conventional plasmid gene expression vectors are still used in the majority of laboratories, mainly due to their technical simplicity and efficient transfection rates in various types of cells.

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