Drew Sowersby - Google+ - Real-Time Experiment # 1 I am going to try and expe... - 0 views
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Erich Feldmeier on 30 Jan 12"I am going to try and experiment within an experiment today. I cloned a couple genes from yeast and have been spending months now trying to get them into several vectors for my combinatorial overexpression project. However, as it goes with wet messy biology, this seemingly simple process has been fighting me tooth and nail. I have retrieved one good clone out of 56 minipreps (plasmid construct isolation) and tried several ligations. So I have tripled down this week. In the picture here, I have 32 tubes, each with 5 individual E. coli colonies. That is a grand total of 160 isolates. 1.5 mL of cells have been transferred from overnight cultures and I am about to spin, lyse, and retrieve."