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katherine-medina

Plants | Free Full-Text | Expression of a Stilbene Synthase Gene from the Vitis labrusc... - 0 views

www.mdpi.com/770

polyphenols stilbene plants polyphenol-transfer HSR-2023 botany tobacco grapes

shared by katherine-medina on 28 Jun 23 - No Cached
  • Stilbenes are found in plants of different families, but the best studied stilbene is resveratrol (3,5,4′-trihydroxystilbene) isolated from grape plants.
  • Resveratrol synthesis in transgenic plants did not always render them fully resistant to one or another pathogen.
  • The aim of our investigation was to isolate a VlvSTS stilbene synthase gene from the hybrid grape V. labrusca x V. vinifera L. and obtain transgenic tobacco plants overexpressing the gene to study their resistance to a number of bacterial and fungal pathogens, among them bacteria as E. carotovora and fungi as B. cinerea, F. oxysporum, F. sporotrichioides, F. culmorum.
  • ...16 more annotations...
  • by PCR. F
    • katherine-medina
      katherine-medina on 28 Jun 23
       
      PCR is a method with which scientists are able to amplify a gene
  • Based on the results of the VlvSTS gene transcription analysis, seven transgenic tobacco lines were selected for further experiments.
  • Expression of the VlvSTS gene in tobacco plants led to a significant increase in the pollen grain size, but with a tendency to a decrease in the total number of pollen grains per anther
    • katherine-medina
      katherine-medina on 28 Jun 23
       
      Too much of that gene leads to a decrease of fertility.
  • In all tests with E. carotovora bacteria, transgenic plants expressing the VlvSTS gene demonstrated a significantly higher resistance versus control plants
    • katherine-medina
      katherine-medina on 28 Jun 23
       
      interesting
  • The leaf involvement was significantly smaller in transgenic plants expressing the VlvSTS gene.
  • Overexpression of the VlvSTS gene reduced the corolla pigmentation in transgenic plants.
    • katherine-medina
      katherine-medina on 28 Jun 23
       
      Why did the overexpression of that gene reduce the corolla pgmentation.
  • binary vector
    • katherine-medina
      katherine-medina on 28 Jun 23
       
      like a DNA transfer
  • showed a significant fertility reduction,
  • For the first time it was shown that plants expressing the VlvSTS gene had enhanced resistance to the bacterial pathogen E. carotovora subsp. carotovora B15.
    • katherine-medina
      katherine-medina on 28 Jun 23
       
      Interesting that it took this long for it to be shown that this specific stilbene can fight against certain pathogens.
  • Interestingly, resistance correlated with the expression level of the stilbene synthase gene. In plants with a higher gene expression (L10 and L23), the area of Erwinia-caused leaf damage was in a range from 0 to 40% respect to the control, while the leaves of line L24 were affected up to 60–100%.
  • hile in transgenic lines, the lysis areas were significantly smaller, probably due to the synthesis of the phytoalexin resveratrol.
  • Some studies showed that Arabidopsis plants transformed with wild grape stilbene synthase genes responded to a pathogen attack by activating genes responsible for the synthesis of signaling molecules such as salicylic and jasmonic acids (SA and JA, respectively) [
  • Our data show a decrease in the total flavonoid content in the flower petals but not in the leaves of plants upon expression of the VlvSTS gene.
    • katherine-medina
      katherine-medina on 28 Jun 23
       
      That seems rather odd.
  • We were the first to show that transgenic tobacco plants carrying the VlvSTS gene had a significantly larger pollen grain size and a smaller number of pollen grains per anther. At the same time, the number of fertile pollen grains decreased, especially in the plant line with the highest expression of the VlvSTS gene.
  • For example, in apple plants with the grape stilbene synthase gene Vst1 under its own promoter, the expression of the gene had no effect on pollen development
  • The study showed that the expression of a VlvSTS stilbene syntase gene in tobacco transgenic plants increases their resistance to bacterial pathogen E. carotovora. There was a significant reduction of disease symptoms after infection of leaves by grey mould fungus B. cinerea, but not to Fusarium fungi. We were the first to show that transgenic tobacco plants carrying the VlvSTS gene had a significantly larger pollen grain size and a smaller number of pollen grains per anther. The number of fertile pollen grains decreased, especially in the plant line with the highest expression of the VlvSTS gene. These changes resulted in a decreased weight of seed bolls in the transgenic tobacco lines.
    • katherine-medina
      katherine-medina on 28 Jun 23
       
      Overall, this article is one that I may come back to depending on what path I go down when searching to develop a research topic further.
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