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Sean Nash

CID Bio-Science Digital Plant Canopy Imager - 0 views

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    (OMG- this would be anazing)
katherine-medina

Antioxidants | Free Full-Text | Drought and UV Radiation Stress Tolerance in Rice Is Im... - 0 views

  • . Plant Material, Growth Conditions, and Phenotypic EvaluationWe used the rice (Oryza sativa L.) seeds of the transgenic (OxF3H) and non-transgenic Nogdong cultivars provided by the Plant Molecular Breeding Laboratory, Kyungpook National University, Korea [41]. Rice seeds were sterilized with fungicides overnight and then washed with double distilled water three times. Next, the rice seeds were soaked in water for four days in an incubator in the dark condition at 32 °C, with the water changed after each 24 h as previously reported by [41]. After soaking and successful sprouting, rice seeds were transferred to autoclaved soil and kept in the dark again for three days. After growth, the three week rice seedlings were exposed to light and kept in a greenhouse for further experimentation.
    • katherine-medina
       
      Found ya, this study waited til rice was 3 weeks old!!!
  • 3. Results
  • 2.7
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  • 2.8.
  • 2.9. Chlorophyll
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    "genin converts very rapidly into flavonoids in response to drought and UV-B radiation stress (see Figure 5). The close image of detection of kaempferol and quercetin is represented in Supplementary Figure S3. "
katherine-medina

Sci-Hub | A droplet-based electricity generator with high instantaneous power density. ... - 0 views

    • katherine-medina
       
      We find that when a falling water droplet spreads on the PTFE surface, it bridges the originally disconnected components (the PTFE/ITO and aluminum electrode) into a closed-loop, electrical system Interesting.
    • katherine-medina
       
      Fig. 1 | Design of the DEG. a, Schematic diagram. b, Optical image showing four parallel DEG devices fabricated on a glass substrate. The volume of each droplet is 100.0 μl. c, As individual droplets continue to impinge on the asfabricated device, the amount of charge on the PTFE surface increases gradually and eventually reaches a stable value. d, One hundred commercial LEDs can be powered when one droplet, released from a height of 15.0 cm, is in contact with the device. e, Under the same experimental conditions (for example, the same droplet size and height of release), the output voltage measured from the DEG (in red, with the frequency of impinging droplets being
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    Interesting to see a different method on creating power out of water. I kinda of want to look into the science of water.
Sean Nash

Satellite Monitoring of Biodiversity Moves Within Reach | Current Science Daily - 2 views

  • Data for the study came from the National Ecological Observatory Network (NEON). The network uses standardized methods to collect biodiversity and Earth observation data across the United States which are then made publicly available. NEON imaging spectrometer data collected from research flights have a pixel size of 1x1 meter.
    • Sean Nash
       
      I wonder if any of the imagery they have is: 1. Accessible by us, and 2. Available for our area. If so, it might be as simple as doing plant diversity surveys to look for ground truth in our area (young forests & grasslands) between what is REALLY on the ground here, vs. what the satellite imagery shows.
  • Biodiversity monitoring from space possible via satellite
  • The reflectance of plants is determined by their chemical, anatomical and morphological characteristics, which are important for interactions among plants and with their environment. “Plants with similar traits, as well as closely related species, therefore tend to have similar reflectance spectra,” explains Schweiger.
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  • Spectral diversity calculated in forests with closed canopies and large individual trees matched plant diversity determined on the ground better than spectral diversity calculated in open landscapes dominated by small herbaceous plants and grasses.
katherine-medina

Restoring the activity of the antibiotic aztreonam using the polyphenol epigallocatechi... - 0 views

  • epigallocatechin gallate (EGCG) against multidrug-resistant clinical isolates of Pseudomonas aeruginosa
    • katherine-medina
       
      Epigallocatechin (EGCG) is a type of catechin or a natural phenol antioxidant. It is commonly found in tea leaves, plums, apple skin, and onions. Sidenote this bacteria is found in green tea
  • However, with resistance increasing against many classes of antibiotic, clinicians often use multiple combinations to treat critically ill patients
  • relatively low toxicity of EGCG to human keratinocytes and G. mellonella larvae.
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  • . EGCG was able to restore the activity of aztreonam against MDR P. aeruginosa . The data presented support further evaluation of the aztreonam–EGCG combination and highlight its potential for use in clinical medici
  • Polyphenols
    • katherine-medina
       
      These are bioactive compounds that are found in fruits and leaves of plants. The main focus of this paper is a type of polyphenol.
  • with EGCG in checkerboard assays, susceptibility increased in P. aeruginosa (n=16, 100%), with the combination proving synergistic in all strains tested
    • katherine-medina
       
      Wow. for how much it increased the susceptibility.
  • Another option to restore the activity of aztreonam against bacterial strains with multiple resistance mechanisms would be to use polyphenols
  • he results demonstrate that synergy between aztreonam and EGCG exists [fractional inhibitory concentration indices (FICIs) 0.02-0.5], with the combination affording significantly (P=<0.05) enhanced bacterial killing, with a >3 log10 reduction in colony-forming units ml−1 at 24 h
  • To access synergy between aztreonam and EGCG, checkerboard assays were performed
  • Chemicals, media, bacterial isolates and animals
    • katherine-medina
       
      Really important to look back at these methods because even though it may not be feasible for me to do an experiment like this one, it still has valuable information for me to look at.
  • Synergy was also found between EGCG and the third-generation cephalosporin, cefotaxime
  • with scores of 64 and 56 out of a maximum of 64 for strains PA2 and PA6, respectively.
  • the increased activity may also be due to the inhibition of the non-mevalonate pathway, resulting in increased susceptibility to aztreonam.
  • Overall, the G. mellonella assays demonstrated that the aztreonam–EGCG combination was superior to monotherapy with either agent against every isolate tested, with significantly lower larval mortality rates
  • In conclusion, the results from this study demonstrate that synergy exists between aztreonam and EGCG against MDR clinical strains of P. aeruginosa in vitro and in vivo. EGCG is also able to restore the antibacterial activity of aztreonam to concentrations below the EUCAST susceptibility breakpoint for P. aeruginosa , potentially expanding and extending its useful therapeutic lifespan. Further work should be undertaken to determine if this combination has the potential to treat clinical infections caused by MDR P. aeruginosa .
    • katherine-medina
       
      My final thought for this article is as follows: 1. This brings up a very interesting topic for me to dig into (polyphenols & antibiotics)
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    A gateway article for me to further my search into the scientific realm involving polyphenols that aid antibiotics.
katherine-medina

Frontiers | Cerebrospinal Fluid Pressure-Related Features in Chronic Headache: A Prospe... - 0 views

  • magnetic resonance
  • magnetic resonance
    • katherine-medina
       
      A non-invasive imaging software
  • A respondent to medical treatment (good outcome) was considered a subject who achieved a 50% or greater reduction in number of headache days per month.
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  • The pressure transducer (Transducer kit, Edwards Lifesciences, Irvine, CA, USA) was attached to the hub of needle via a 10-cm long flexible tube without loss of CSF.
  • 93 had abnormal CSF pressure pulsations, with opening pressures ranging from 206 to 385 mm
  • . Group 1 comprised 55 patients, each with an opening pressure below 200 mm H2O
  • Group 2 comprised 56 patients, each with a slight elevation of CSF opening pressure (mean = 231.8
  • Group 3 included 37 patients, all with both elevated CSF opening pressure (mean = 282.2 mm H2O,
  • n Group 3, patients with severe abnormal pressure pulsations and mean pressure >250 mm H2O had the most severe headaches (highest scores on VAS and MIDAS) and associated symptoms (high frequency of: nocturnal headache, postural headache, photopsias, transient visual obscurations).
  • In Group 2, patients with less severe abnormal pressure pulsations and mean pressure >200 mm H2O, had less severe symptoms; in Group 1, patients who had no abnormal pressure pulsations and mean pressures below 200 mm H2O had primary chronic headache disorder (Figure 1).
  • . All of the subjects in the control group, all with opening pressures below 200 mm H2o and normal CSF pressure pulsations, had no headache.
    • katherine-medina
       
      So essentially if the CSF pressure was too low the patient had chronic headache disorder, but if the patients had a higher level of pressure they would suffer from the most painful headaches. (This occurring as nocturnal headaches, postural headaches, and transient visual obstructions.
  • An improvement in the intensity of pain and frequency of headaches was observed following CSF removal in all patients with ICH
  • 69 (74%) out 93 patients achieved ≥50% reduction in headache days.
  • Our results provide evidence that nocturnal and postural headache, and abnormal pressure pulsations are the most common pressure-related features of isolated high CSF pressure in patients with chronic headache
  • o explain this mimicry, we hypothesize that abnormal pulsations in CSF pressure may trigger the same mechanism that cause the pain in a primary headache attack. The mimicry thereby emerges as a variation of pre-existing primary headache; as such, it could be considered a triggered headache rather than a secondary or primary one.
  • The pathogenesis of these abnormal pulsations in CSF pressure (B waves and plateau waves) may be clarified by reference to the theory that CSF pressure is derived from the circulation of cerebral blood and cerebrospinal fluid
    • katherine-medina
       
      Hmmm... I had no idea that there is a theory that states that CSF pressure is derived from the circulation of blood. (I might wanna look into the further)
  • This suggests that CSF pressure assessment by measuring the height of the fluid column in the manometer line connected to a spinal puncture needle may be misleading in the case of headache sufferers.
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    A good study to look at when looking at possible causes of headaches, and more information about CSF.
Sean Nash

SciDraw | Scientific Drawings - 0 views

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    This is a great source for constructing diagrams you will need for your display, presentation, & manuscript
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