Limitation of Cytokinin Export to the Shoots by Nucleoside Transporter ENT3 and Its Lin... - 0 views
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Kylie John on 07 Nov 24"first_pagesettingsOrder Article Reprints Open AccessArticle Limitation of Cytokinin Export to the Shoots by Nucleoside Transporter ENT3 and Its Linkage with Root Elongation in Arabidopsis by Alla Korobova 1,Bulat Kuluev 2,3,Torsten Möhlmann 4,Dmitriy Veselov 1 andGuzel Kudoyarova 1,3,* 1 Laboratory of Plant Physiology, Ufa Institute of Biology, Ufa Federal Research Centre, RAS, 450054 Ufa, Russia 2 Institute of Biochemistry and Genetics, Ufa Federal Research Centre, RAS, 450054 Ufa, Russia 3 Biological Department, Bashkir State University, 450076 Ufa, Russia 4 Department of Biology, University of Kaiserslautern, 67663 Kaiserslautern, Germany * Author to whom correspondence should be addressed. Cells 2021, 10(2), 350; https://doi.org/10.3390/cells10020350 Submission received: 18 December 2020 / Revised: 1 February 2021 / Accepted: 6 February 2021 / Published: 8 February 2021 (This article belongs to the Special Issue Local and Systemic Signals of Macronutrient and Water Availability Regulating Root Growth and Development) Downloadkeyboard_arrow_down Browse Figures Versions Notes Abstract The trans-membrane carrier AtENT3 is known to transport externally supplied cytokinin ribosides and thus promote uptake by cells. However, its role in distributing either exogenous or endogenous cytokinins within the intact plant has not hitherto been reported. To test this, we used ent3-1 mutant Arabidopsis seedlings in which the gene is not expressed due to a T-DNA insertion, and examined the effect on the concentration and distribution of either endogenous cytokinins or exogenous trans-zeatin riboside applied to the roots. In the mutant, accumulation of endogenous cytokinins in the roots was reduced and capacity to deliver externally supplied trans-zeatin riboside to the shoots was increased suggesting involvement of equilibrative nucleoside (ENT) transporter in the control of cytokinin distribution in the plants. Roots of ent3-1 were longer in the mutant in association with t
