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ScienceDirect.com - Vaccine - Intranasal and intramuscular immunization with Baculoviru... - 0 views

www.sciencedirect.com/...S0264410X10009412
shared by Sarah Muncy on 27 Sep 12 - No Cached
  • An anti-malarial transmission-blocking vaccine (TBV) that prevents fertilization and/or ookinete/oocyst development within the mosquito is an attractive strategy to limit the transmission of malaria
  • The present study used this system to generate a Plasmodium vivax transmission-blocking immunogen (AcNPV-Dual-Pvs25).
  • Plasmodium vivax
  • ...10 more annotations...
  • A variety of expression vectors (e.g., Escherichia coli, Pichia pastoris and DNA) have been used to express Pvs25 protein which has been administered alone or in combination with adjuvants
  • To date these studies suggest that the recombinant protein currently requires both not only linear, but conformation dependent epitopes, and a strong adjuvant to induce transmission-blocking antibodies.
  • Intranasal and intramuscular immunization with Baculovirus Dual Expression System-based Pvs25 vaccine substantially blocks Plasmodium vivax transmission
  • Recently, we have developed a new vaccine vector system based on the baculovirus Autographa californica nucleopolyhedrosis virus (AcNPV) termed the “Baculovirus Dual Expression System”, which drives expression of vaccine candidate antigens by a dual promoter that consists of tandemly arranged baculovirus-derived polyhedrin and mammalian-derived CMV promoters. It has been shown that AcNPV, an enveloped double-stranded DNA virus that naturally infects insects, possesses strong adjuvant properties that can activate dendritic cell-mediated innate immunity
  • Mucosal vaccines have several attractive features compared with parenteral vaccines (e.g., safety, cost-effectiveness and ease of administration), but studies on their use have been limited almost exclusively to protection against mucosally transmitted pathogens. We provide evidence that i.n. immunization is a feasible alternative for preventing malaria, which is transmitted through non-mucosal routes
  • These results are consistent with our previous work showing that intranasal immunization with the baculovirus-based vaccine induced strong systemic humoral immune responses with high titres of antigen-specific antibodies and conferred complete protection against malaria blood-stage challenge
  • which can induce immunological memory against heterologous antigens in a rodent model; however, it is precluded from clinical use due to its enterotoxicity and potential hazardous effects on olfactory nerves [22]. In contrast, a baculovirus-based delivery system may offer an attractive immunization method, as AcNPV exhibits low cytotoxicity and is incapable of replication in mammalian cells
  • The data described here adds to previously presented data showing the significant potential of the baculovirus dual expression system against the blood stages of the parasite
  • but also demonstrates clearly its ability to induce antibodies against the ookinete surface protein Pvs25, and to elicit a transmission-blocking immune response against the P. vivax isolates from endemic areas, and a transgenic rodent malaria parasite model in preliminary studies.
  • One was SMFA on peripheral blood from P. vivax infected patients.
  • Sarah Muncy on 27 Sep 12
     
    Reference paper #2. Gave me information on malaria and baculoviruses.
1More

Genomic Characterization of a Novel Virus of the Family Tymoviridae Isolated from Mosqu... - 3 views

www.plosone.org/...10.1371%2Fjournal.pone.0039845 virus tymoviridae virology
shared by jiyoung yoon on 27 Sep 12 - No Cached
  • jiyoung yoon on 27 Sep 12
     
    in this article we can know about unique novel tymoviridae virus. they are characterising about this new virus. one of the most unique feature about them is they can replicate even in mosquitoes!in consider that no mosquitoes have been reported as vectores of plant viruse,this research is quite suprising thing! but something i want to recommend is ...first At the result, it said cell lysates did not react with antisera to known only a few arboviruses of the genera. And said this virus is an uncommon arbovirus . I think the author should define more specially about the subject. and second, this research said that CuTLV virus may be transferred to male mosquitoes feed on plant juices and can started to replicate in the insect also. Because marifviruses can potentially replicate both in plants and in insects but, I can't figure out how this viruse can move into human,(animal) different kinds of species , can make a disease even by male ! I think the writer should more explain about that route. the last thing is There is many pictures .but some of them ,like figure 4&figure 5, are too massy to be hard recognized or unneeded thing. So I think they need more arrangement about picture.
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Rapid amplification of 5' complementary DNA ends... [Nat Methods. 2005] - PubMed - NCBI - 0 views

www.ncbi.nlm.nih.gov/...16145794
shared by jiyoung yoon on 27 Sep 12 - No Cached
  • jiyoung yoon on 27 Sep 12
     
    i want to know about More detailed work of 5'-RACE . from that research, i could know that 5'-race make a complementary connection with second primer required for PCR mRNA so that provides a primer-binding site upstream of the unknown 5' sequence of the target mRNA !
1More

EBSCOhost: Isolates of Liao Ning Virus from Wild-Caught Mosquitoes in the Xinjiang Pro... - 1 views

web.ebscohost.com/...pdfviewer virology genomics
shared by jiyoung yoon on 27 Sep 12 - No Cached
  • jiyoung yoon on 27 Sep 12
     
    from that research, i could know about what kinds of other virus which can use mosquitoes for their movement.
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EBSCOhost: Isolates of Liao Ning Virus from Wild-Caught Mosquitoes in the Xinjiang Pro... - 2 views

web.ebscohost.com/...pdfviewer virology
shared by jiyoung yoon on 27 Sep 12 - No Cached
  • jiyoung yoon on 27 Sep 12
     
    from that writing i could find the anwer "why rna virus can maintain their big size?". because they have really complex rigid structure
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Enterovirus and Diabetes type 1 - 0 views

www.ncbi.nlm.nih.gov/...PMC3033438 virology
shared by kchenvert09 on 26 Sep 12 - No Cached
  • kchenvert09 on 26 Sep 12
     
    This paper deals with the possibility that a virus may be a cause for diabetes type 1
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ScienceDirect.com - Journal of Bioscience and Bioengineering - Efficient production of ... - 0 views

www.sciencedirect.com/...S138917231000191X
shared by Sarah Muncy on 26 Sep 12 - No Cached
  • Sarah Muncy on 26 Sep 12
     
    This article about baculoviruses goes into how not only can baculoviruses make simple proteins, or express angitens of forgein substances, they can also make pieces of antibodies!
12More

ScienceDirect.com - Vaccine - Hemagglutinin Displayed Baculovirus Protects Against High... - 0 views

www.sciencedirect.com/...S0264410X10011850
shared by Sarah Muncy on 26 Sep 12 - No Cached
    • Sarah Muncy
      Sarah Muncy on 26 Sep 12
       
      So, the baculovirus on TOP of having the H5HA on it, can also get the immune system to kick in better?
  • It is remarkable that low doses (103pfu/mouse) of BVs act as an effective adjuvant [41]. Therefore, reducing BV concentration and elongating vaccination intervals may prevent memory responses to BV administration
  • scanning densitometry
  • ...8 more annotations...
  • Foreign immunogens or peptides can be displayed on the envelope of AcMNPV by fusion with the baculovirus major envelope protein gp64
  • Baculoviruses have strong adjuvant activity to promote humoral and cellular immune responses against coadministered antigens, activate dendritic cells maturation, induce the production of cytokines, chemokines, and type I IFNs
  • There are two influenza vaccine approaches licensed in the US; the inactivated, split vaccine and the live-attenuated virus vaccine. Inactivated vaccines can efficiently induce humoral immune responses but generally only poor cellular immune responses.
  • Therefore, influenza HA can be displayed on the surface of baculovirus
  • virus-like particle (VLP)
  • Even though cellular immune responses cannot confer sterilizing immunity, they are able to reduce the severity of infection and lower morbidity and mortality rates [47], and antigen-specific memory T cells are able to rapidly respond to a secondary virus infection [45]. Furthermore, cellular immune responses to the conserved epitopes contained in vaccines may provide cross-protective immunity against different subtypes of influenza virus infection
  • To confirm that each HA was incorporated on the envelope of baculoviruses, supernatants from infected Sf9 cells were used to perform hemagglutination assay
  • Most BV display strategies rely on gp64 protein which is the major envelope protein of baculovirus.
  • Sarah Muncy on 26 Sep 12
     
    This paper gave me a better understanding of some aspects of my focal paper that were unclear. How to test for HA, and how baculoviruses may be adjuvants in addition to expression vectors.
1More

PLOS ONE: Safety and Immunogenicity of H5N1 Influenza Vaccine Based on Baculovirus Surf... - 0 views

www.plosone.org/...5E58993766A3A1256696729D429B79
shared by Sarah Muncy on 26 Sep 12 - No Cached
  • Sarah Muncy on 26 Sep 12
     
    This paper gives a descriptive about how they were able to make a candidate vaccine for influenza that is really cheap/safe and very effective using Bombyx mori caterpillars as bioreactors to get needed proteins.
1More

Cooperative interactions in the West Nile virus mutant swarm - 0 views

www.ncbi.nlm.nih.gov/...PMC3358237 virology population biology genomics genetics
shared by Casey Finnerty on 25 Sep 12 - No Cached
  • In addition, our data demonstrate that increasing levels of co-infection can lead to widespread strain complementation, which acts to maintain high levels of phenotypic and genetic diversity and potentially slow selection for individual variants. Lastly, we show that cooperative interactions may lead to swarm fitness levels which exceed the relative fitness levels of any individual genotype.
1More

Quasispecies diversity determines pathogenesis throug... [Nature. 2006] - PubMed - NCBI - 0 views

www.ncbi.nlm.nih.gov/...16327776 virology genomics population biology genetics
shared by Casey Finnerty on 25 Sep 12 - No Cached
  • Analysis of viruses isolated from brain provides direct evidence for complementation between members in the quasispecies, indicating that selection indeed occurs at the population level rather than on individual variants.
1More

Introduction of the Six Major Genomic Deletions of Modified Vaccinia Virus Ankara (MVA)... - 0 views

www.ncbi.nlm.nih.gov/...PMC2937755 virology genomics
shared by Casey Finnerty on 25 Sep 12 - No Cached
  • In mice, CVA mutants with up to three deletions showed slightly enhanced virulence, suggesting that gene deletion in replicating vaccinia virus (VACV) can result in gain of fitness in vivo.
1More

VIRULENCE NOT ONLY COSTS BUT ALSO BENEFITS THE TRANSMISSION OF A FUNGAL VIRUS - Bryner ... - 0 views

onlinelibrary.wiley.com/...full virology evolution
shared by Casey Finnerty on 25 Sep 12 - No Cached
  • Casey Finnerty on 25 Sep 12
     
    This paper provides experimental support for the ideas of Anderson and May on host-parasite coevolution.
1More

Coevolution of hosts and parasites. [Parasitology. 1982] - PubMed - NCBI - 0 views

www.ncbi.nlm.nih.gov/...6755367 virology
shared by Casey Finnerty on 25 Sep 12 - No Cached
  • Casey Finnerty on 25 Sep 12
     
    This paper is a seminal publication in our understanding of host-parasite coevolution. The authors suggest that virulence will be maintained if necessary for parasite transmission.
1More

ScienceDirect.com - Virology - Internally deleted WNV genomes isolated from exotic bird... - 0 views

www.sciencedirect.com/...S004268221200075X virology WNV genomics
shared by Casey Finnerty on 25 Sep 12 - No Cached
  • In mosquitoes, the shortened WNV genomes reduced infection and dissemination rates, and virus titers overall, and were not detected in legs or salivary secretions at 14 or 21 days post-infection. In mice, inoculation with internally deleted genomes did not attenuate pathogenesis relative to full-length or infectious clone derived virus, and shortened genomes were not detected in mice at the time of death.
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