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"I am going to try and experiment within an experiment today. I cloned a couple genes from yeast and have been spending months now trying to get them into several vectors for my combinatorial overexpression project. However, as it goes with wet messy biology, this seemingly simple process has been fighting me tooth and nail. I have retrieved one good clone out of 56 minipreps (plasmid construct isolation) and tried several ligations. So I have tripled down this week. In the picture here, I have 32 tubes, each with 5 individual E. coli colonies. That is a grand total of 160 isolates. 1.5 mL of cells have been transferred from overnight cultures and I am about to spin, lyse, and retrieve."

"Leah Pyter: Well basically what we know is that patients with cancer have a higher likelihood of also developing depression at some point in their disease progression, so whether that occurred before and is predisposing them to cancer, or it's due to the tumours themselves, or other aspects of having the disease, we don't know. We were only studying right now whether the cancer itself can cause depression. Chris Smith: How could a tumour trigger depression, because a tumour can occur anywhere in the body, therefore at the remote sites in the brain, so how could it trigger changes in brain activity? Leah Pyter: Sure, well what we hypothesized was that the tumours themselves can produce cytokines which has been shown before. Chris Smith: These are inflammatory chemicals that drive the immune system? Leah Pyter: Right, exactly! And there is also a pile of research on how cytokines can access the brain specifically regions of the brain that are associated with depression and anxiety and emotional behaviours, and they can access the brain both tumourally through the blood, or neurally through the vegas nerves. "

"During wakefulness, we are always conscious of ourselves. In sleep, however, we are not. But there are people, known as lucid dreamers, who can become aware of dreaming during sleep. Studies employing magnetic resonance tomography (MRT) have now been able to demonstrate that a specific cortical network consisting of the right dorsolateral prefrontal cortex, the frontopolar regions and the precuneus is activated when this lucid consciousness is attained. All of these regions are associated with self-reflective functions. This research into lucid dreaming gives the authors of the latest study insight into the neural basis of human consciousness."

"An IEEE Spectrum contributing editor, Charette is a self-described "risk ecologist" who investigates the impact of risk on technology and society. His interest is both professional and personal: He's a 33-year member of the IEEE Computer Society and has two daughters who are contemplating STEM careers. "Now I can give better career advice to my daughters," he says"

Many newly diagnosed prostate cancers present as low Gleason score tumors that require no treatment intervention. Distinguishing the many indolent tumors from the minority of lethal ones remains a major clinical challenge. We now show that low Gleason score prostate tumors can be distinguished as indolent and aggressive subgroups on the basis of their expression of genes associated with aging and senescence. Using gene set enrichment analysis, we identified a 19-gene signature enriched in indolent prostate tumors. We then further classified this signature with a decision tree learning model to identify three genes-FGFR1, PMP22, and CDKN1A-that together accurately predicted outcome of low Gleason score tumors. Validation of this three-gene panel on independent cohorts confirmed its independent prognostic value as well as its ability to improve prognosis with currently used clinical nomograms. Furthermore, protein expression of this three-gene panel in biopsy samples distinguished Gleason 6 patients who failed surveillance over a 10-year period. We propose that this signature may be incorporated into prognostic assays for monitoring patients on active surveillance to facilitate appropriate courses of treatment.

Scientists had thought that there was a single species of housecat-sized Brazilian tigrina. However, the molecular data now show that tigrina populations in northeastern versus southern Brazil are completely separate, with no evidence of interbreeding between them. As such, they are best described as two distinct species.

"Biohacking: The next great wave of innovation The hacker culture that launched the computing revolution is now taking root in the bio space"

"The informatics portal io-port.net offers fast and convenient access to about more than two million publications in informatics and related subject areas from all over the world. All information, which up to then had been stored in various data sources, has been consolidated and is now available from one source. All steps required for information retrieval are available via an easy-to-use, powerful interface."

Bacteria are often social creatures. Suspended in colonies of varying shapes and sizes, these microbes communicate with their brethren and even other bacterial species - interactions that can sometimes make them more deadly or more resistant to antibiotics. Now, bacterial colonies sculpted into custom shapes by a 3-D printer could be a key to understanding how some antibiotic-resistant infections develop. The new technique uses methods similar to those employed by commercial 3-D printers, which extrude plastic, to create gelatin-based bacterial breeding grounds. These microbial condos can be carved into almost any three-dimensional shape, including pyramids and nested spheres.

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Now you can avail the best quality sediment control products to minimize soil deterioration from Coastline Sediment Control. These products and techniques are used to reduce the quantity of sediment leaving a site.

"Interactive climbs are now possible for the first time in the history of alpinism. Experience the most famous routes of the world in a 360° view. Please use a smart phone or a tablet of the latest generation or an up-to-date browser for a perfect 360° experience."

For more than a century, neuroscientists believed that the brains of humans and other mammals differed from the brains of other animals, such as birds (and so were presumably better). Researchers have now found that a comparable region in the brains of chickens concerned with analyzing auditory inputs is constructed similarly to that of mammals.

Drug-resistant bacteria kills, even in top hospitals. But now tough infections like staph and anthrax may be in for a surprise. Nobel-winning chemist Kary Mullis, who watched a friend die when powerful antibiotics failed, unveils a radical new cure that shows extraordinary promise.

The US Space Shuttle is down to its final few flights. The space shuttle has been flying for 29 years now and is coming to a close. The Shuttle Program has accomplished much in these 29 years.

By working with the simplest amino acids and elementary RNAs, physicists led by Rockefeller University's Albert J. Libchaber, head of the Laboratory of Experimental Condensed Matter Physics, have now generated the first theoretical model that shows how a coded genetic system can emerge from an ancestral broth of simple molecules.

As if they weren't considered beastly enough, black holes can dive into nearby stars and devour them from the inside out, scientists now suggest. Such invasions by such black holes could help explain the most powerful explosions in the universe, gamma-ray bursts, whose origins remain elusive.

WHAT do earthquakes, spinning stellar remnants, bright space objects and a host of other natural phenomena have in common? Some of their properties conform to a curious and little known mathematical law, which could now find new uses.

By dampening the immune system, cannabis provides relief from inflammatory diseases, but it also increases the risk of infections. Now we know how