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Custom Cloning: Hanbio can clone your sequence into the vector of your choice. We have a wide range of vectors to choose from, including the conventional pUC57, pUC57-Kan, and 6 other high-copy cloning plasmids. We also offer commonly used commercial vectors such as the pcDNA3.1 series, pET series, among others. Alternatively, you can provide your specified vector for us to assist in the cloning construction.

Lentivirus vector is a gene therapy vector developed on the base of HIV-1 (human immunodeficiency virus type 1). Lentivirus vector is a powerful tool for introduction of exogenous genes, with the advantages of wide infection spectrum, effective infection and long-term stable expression of exogenous genes.

The crispr cas 9 plasmid system is a gene editing technology based on the modification of adaptive immunity mechanisms in prokaryotes. It consists of two modules, one is the gRNA and its backbone that recognise specific DNA sequences, the other is Cas9, a protein that binds and edits DNA. Since its invention, the technology has developed rapidly. Nowadays, cas9 grna plasmid and its variants are widely used in various fields, such as editing, detecting, labelling, and imaging of nucleic acids in live cells. The technology has revolutionised the way basic life sciences and biomedicine are researched due to its efficiency and ease of use in different species. The inventor of the technology was also awarded the 2020 Nobel Prize in Chemistry. Today the crispr cas virus system is still thriving, with broad prospects and deep potential both in basic science research and in clinical applications such as gene therapy and cell therapy.

Seamless clone technique from HANBIO is a new, fast and simple cloning method, which can insert one or more target DNA fragments at any site of the plasmid without any restriction enzymes and ligases. Breaking through the traditional double enzyme digestion and linking, the recombinant vector of high efficiency cloning can be obtained by one-step recombinant method, which greatly improves the working efficiency.

To create a stable cell line generation lentivirus, cells are typically transduced with lentiviral particles containing the desired gene of interest. The lentiviral vector integrates into the host cell's genome, leading to stable expression of the gene in the cell line. This allows researchers to study the effects of the gene of interest in a controlled and reproducible manner.