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To create a stable cell line generation lentivirus, cells are typically transduced with lentiviral particles containing the desired gene of interest. The lentiviral vector integrates into the host cell's genome, leading to stable expression of the gene in the cell line. This allows researchers to study the effects of the gene of interest in a controlled and reproducible manner.

Luciferase is a general term for enzymes that can produce bioluminescence in nature, with the most representative ones are F-Luciferase from fireflies and R-Luciferase from renilla. Under the catalysis of luciferase, luciferin can efficiently convert to oxidized luciferin and emit strong fluorescence at the same time. Therefore, researchers used luciferase to develop a highly sensitive and convenient gene reporter system. Currently, this system is widely applied in various fields, including transcription factor binding site and promoter activity analysis, RNA degradation, signal transduction, drug screening, and in vivo animal imaging.