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The crispr cas 9 plasmid system is a gene editing technology based on the modification of adaptive immunity mechanisms in prokaryotes. It consists of two modules, one is the gRNA and its backbone that recognise specific DNA sequences, the other is Cas9, a protein that binds and edits DNA. Since its invention, the technology has developed rapidly. Nowadays, cas9 grna plasmid and its variants are widely used in various fields, such as editing, detecting, labelling, and imaging of nucleic acids in live cells. The technology has revolutionised the way basic life sciences and biomedicine are researched due to its efficiency and ease of use in different species. The inventor of the technology was also awarded the 2020 Nobel Prize in Chemistry. Today the crispr cas virus system is still thriving, with broad prospects and deep potential both in basic science research and in clinical applications such as gene therapy and cell therapy.

Custom Cloning: Hanbio can clone your sequence into the vector of your choice. We have a wide range of vectors to choose from, including the conventional pUC57, pUC57-Kan, and 6 other high-copy cloning plasmids. We also offer commonly used commercial vectors such as the pcDNA3.1 series, pET series, among others. Alternatively, you can provide your specified vector for us to assist in the cloning construction.

Luciferase is a general term for enzymes that can produce bioluminescence in nature, with the most representative ones are F-Luciferase from fireflies and R-Luciferase from renilla. Under the catalysis of luciferase, luciferin can efficiently convert to oxidized luciferin and emit strong fluorescence at the same time. Therefore, researchers used luciferase to develop a highly sensitive and convenient gene reporter system. Currently, this system is widely applied in various fields, including transcription factor binding site and promoter activity analysis, RNA degradation, signal transduction, drug screening, and in vivo animal imaging.

Circular RNAs (circRNAs) are a type of non-coding RNA molecules that lack a 5' cap and a 3' poly(A) tail and form a circular structure with covalent bonds. Distinct from traditional linear RNAs, circRNAs possess a closed circular structure and are abundantly present in the eukaryotic transcriptome. The majority of circRNAs are composed of exon sequences and exhibit conservation across different species, as well as tissue-specific and expression patterns of developmental stage-specific.

Long Noncoding RNA as Modular Scaffold of Histone Modification Complex Long intergenic non protein coding rna is a class of long non coding rna in plants molecule with a length of more than 200nt, which is a by-product of RNA polymerase II transcription. It can regulate gene expression at various levels (epigenetic regulation, transcriptional regulation and post-transcriptional regulation, etc.). It is a research hotspot of genetics.

Seamless clone technique from HANBIO is a new, fast and simple cloning method, which can insert one or more target DNA fragments at any site of the plasmid without any restriction enzymes and ligases. Breaking through the traditional double enzyme digestion and linking, the recombinant vector of high efficiency cloning can be obtained by one-step recombinant method, which greatly improves the working efficiency.