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Matti Narkia

Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo - Nut... - 0 views

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    Anticancer properties of Ganoderma lucidum methanol extracts in vitro and in vivo.
    Harhaji Trajković LM, Mijatović SA, Maksimović-Ivanić DD, Stojanović ID, Momcilović MB, Tufegdzić SJ, Maksimović VM, Marjanović ZS, Stosić-Grujicić SD.
    Nutr Cancer. 2009;61(5):696-707.
    PMID: 19838944
    DOI: 10.1080/01635580902898743

    Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.
Matti Narkia

Lingzhi mushroom - Wikipedia, the free encyclopedia - 0 views

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    "Língzhī (traditional Chinese: 靈芝; simplified Chinese: 灵芝; Japanese: reishi; Korean: yeongji, hangul: 영지) is the name for one form of the mushroom Ganoderma lucidum, and its close relative Ganoderma tsugae. Ganoderma lucidum enjoys special veneration in Asia, where it has been used as a medicinal mushroom in traditional Chinese medicine for more than 4,000 years, making it one of the oldest mushrooms known to have been used in medicine.

    Lingzhi may possess anti-tumor, immunomodulatory and immunotherapeutic activities, supported by studies on polysaccharides, terpenes, and other bioactive compounds isolated from fruiting bodies and mycelia of this fungus (reviewed by R. R. Paterson[4] and Lindequist et al.[7]). It has also been found to inhibit platelet aggregation, and to lower blood pressure (via inhibition of angiotensin-converting enzyme[8]), cholesterol and blood sugar.[9]

    Laboratory studies have shown anti-neoplastic effects of fungal extracts or isolated compounds against some types of cancer. In an animal model, Ganoderma has been reported to prevent cancer metastasis,[10] with potency comparable to Lentinan from Shiitake mushrooms.[11]

    The mechanisms by which G. lucidum may affect cancer are unknown and they may target different stages of cancer development: inhibition of angiogenesis (formation of new, tumor-induced blood vessels, created to supply nutrients to the tumor) mediated by cytokines, cytoxicity, inhibiting migration of the cancer cells and metastasis, and inducing and enhancing apoptosis of tumor cells
Matti Narkia

Glucose restriction can extend normal cell lifespan and impair precancerous cell growth... - 1 views

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    Glucose restriction can extend normal cell lifespan and impair precancerous cell growth through epigenetic control of hTERT and p16 expression.
    Li Y, Liu L, Tollefsbol TO.
    FASEB J. 2009 Dec 17. [Epub ahead of print]
    PMID: 20019239
    doi: 10.1096/fj.09-149328

    Cancer cells metabolize glucose at elevated rates and have a higher sensitivity to glucose reduction. However, the precise molecular mechanisms leading to different responses to glucose restriction between normal and cancer cells are not fully understood. We analyzed normal WI-38 and immortalized WI-38/S fetal lung fibroblasts and found that glucose restriction resulted in growth inhibition and apoptosis in WI-38/S cells, whereas it induced lifespan extension in WI-38 cells. Moreover, in WI-38/S cells glucose restriction decreased expression of hTERT (human telomerase reverse transcriptase) and increased expression of p16(INK4a). Opposite effects were found in the gene expression of hTERT and p16 in WI-38 cells in response to glucose restriction. The altered gene expression was partly due to glucose restriction-induced DNA methylation changes and chromatin remodeling of the hTERT and p16 promoters in normal and immortalized WI-38 cells. Furthermore, glucose restriction resulted in altered hTERT and p16 expression in response to epigenetic regulators in WI-38 rather than WI-38/S cells, suggesting that energy stress-induced differential epigenetic regulation may lead to different cellular fates in normal and precancerous cells. Collectively, these results provide new insights into the epigenetic mechanisms of a nutrient control strategy that may contribute to cancer therapy as well as antiaging approaches.
Matti Narkia

Induction of Ovarian Cancer Cell Apoptosis by 1,25-Dihydroxyvitamin D3 through the Down... - 0 views

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    Induction of ovarian cancer cell apoptosis by 1,25-dihydroxyvitamin D3 through the down-regulation of telomerase.
    Jiang F, Bao J, Li P, Nicosia SV, Bai W.
    J Biol Chem. 2004 Dec 17;279(51):53213-21. Epub 2004 Oct 12.
    PMID: 15485861
    doi: 10.1074/jbc.M410395200

    Overall, the study suggests that the down-regulation of telomerase activity by 1,25(OH)2VD3 and the resulting cell death are important components of the response of OCa cells to 1,25(OH)2VD3-induced growth suppression.

    Progressive shortening of telomere associated with cell divisions limits the life span of normal cells and eventually leads to senescence. To become immortal, human cancers including OCa are invariably associated with activation of mechanism that maintains telomere length. Approximately 85-90% of cancers show reactivation of telomerase. The present study shows that telomerase in OCa cells is down-regulated by 1,25(OH)2VD3. Down-regulation of telomerase is due to decreased stability of hTERT mRNA rather than VDRE-mediated transcriptional repression through the putative VDRE present in the regulatory region of the hTERT gene.

    It is known that the inhibition of telomerase may lead to a phenotypic lag during which cells would continue to divide until the point at which the telomeres became critically short. This phenomenon may explain why the apoptotic induction by 1,25(OH)2VD3 needs the treatment for more than 6 days. As mentioned in the results, no detectable shortening of telomeric repeats was observed in parental OVCAR3 cells after 9 days of treatment with 1,25(OH)2VD3 (Fig. 4D). This is likely due to the fact that the short telomere (about 3 kb) in OVCAR3 cells is very close to the minimal length required for survival and that cells with detectably shorter telomere may have been selected against apoptosis. It has been shown that transformed human cells enter crisis once the terminal restriction fragment of the telomere reaches a length of about 4 kb. This is insufficient to protect chro
Matti Narkia

Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependen... - 0 views

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    Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells.
    Mantena SK, Sharma SD, Katiyar SK.
    Mol Cancer Ther. 2006 Feb;5(2):296-308.
    PMID: 16505103
    doi: 10.1158/1535-7163.MCT-05-0448

    The effectiveness of berberine in checking the growth of androgen-insensitive, as well as androgen-sensitive, prostate cancer cells without affecting the growth of normal prostate epithelial cells indicates that it may be a promising candidate for prostate cancer therapy.

    The evaluation of ancient herbal medicines may indicate novel strategies for the treatment of prostate cancer, which remains the leading cause of cancer-related deaths in American men (1). In our present investigation, we show that a naturally occurring isoquinoline alkaloid, berberine, significantly inhibits the proliferation and reduces the viability of DU145 and PC-3 as well as LNCaP cells (Fig. 1), which suggests that berberine may be an effective chemotherapeutic agent against both androgen-sensitive and androgen-insensitive prostate cancer cells. Importantly, we found that berberine did not exhibit toxicity to nonneoplastic human prostate epithelial cells under the conditions used, except for a moderate reduction in cell viability at higher concentrations when cells were treated in vitro for an extended period of time.

    In conclusion, the results of the present study indicate that berberine inhibits proliferation and induces G1-phase arrest and apoptosis in human prostate cancer cells but not in normal human prostate epithelial cells. In addition, we provide mechanistic evidence that berberine-induced apoptosis in prostate carcinoma cells, particularly hormone-refractory prostate carcinoma cells, is mediated through enhanced expression of Bax, disruption of the mitochondrial membrane potential, and activation of caspase-3.
Matti Narkia

Modulatory effects of EPA and DHA on proliferation and apoptosis of pancreatic cancer c... - 0 views

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    It was concluded that omega-3 fatty acid could inhibit the proliferation of pancreatic cancer cell line SW1990 cells and promote their apoptosis. The down-regulation of the cyclin E expression by omega-3 fatty acid might be one of the mechanisms for its anti-tumor effect on pancreatic cancer.

    Modulatory effects of EPA and DHA on proliferation and apoptosis of pancreatic cancer cells.
    Zhang W, Long Y, Zhang J, Wang C.
    J Huazhong Univ Sci Technolog Med Sci. 2007 Oct;27(5):547-50.
    PMID: 18060632
Matti Narkia

Anticancer properties of oxidation products of docosahexaenoic acid - [Chem Phys Lipids... - 0 views

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    Anticancer properties of oxidation products of docosahexaenoic acid.
    Siddiqui RA, Harvey K, Stillwell W.
    Chem Phys Lipids. 2008 May;153(1):47-56. Epub 2008 Feb 23. Review.
    PMID: 18343223
Matti Narkia

How spicy foods can kill cancers - BBC NEWS | Health - 0 views

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    Scientists have discovered the key to the ability of spicy foods to kill cancer cells.
    They found capsaicin, an ingredient of jalapeno peppers, triggers cancer cell death by attacking mitochondria - the cells' energy-generating boiler rooms.
Matti Narkia

CD40L - a multipotent molecule for tumor therapy. - Bentham Science Publishers - Endocr... - 0 views

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    CD40L - a multipotent molecule for tumor therapy.
    Loskog A, Tötterman TH.
    Endocr Metab Immune Disord Drug Targets. 2007 Mar;7(1):23-8. Review.
    PMID: 17346201
Matti Narkia

White button mushroom (Agaricus bisporus) exhibits antiproliferative and proapoptotic p... - 0 views

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    White button mushroom (Agaricus bisporus) exhibits antiproliferative and proapoptotic properties and inhibits prostate tumor growth in athymic mice.
    Adams LS, Phung S, Wu X, Ki L, Chen S.
    Nutr Cancer. 2008;60(6):744-56.
    PMID: 19005974
Matti Narkia

Exercise affects platelet-impeded antitumor cytotoxicity of natural killer cell. - [Med... - 0 views

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    Exercise affects platelet-impeded antitumor cytotoxicity of natural killer cell.
    Wang JS, Chung Y, Chow SE.
    Med Sci Sports Exerc. 2009 Jan;41(1):115-22.
    PMID: 19092699
Matti Narkia

White Button Mushroom (Agaricus Bisporus) Exhibits Antiproliferative and Proapoptotic P... - 0 views

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    White button mushroom (Agaricus bisporus) exhibits antiproliferative and proapoptotic properties and inhibits prostate tumor growth in athymic mice.
    Adams LS, Phung S, Wu X, Ki L, Chen S.
    Nutr Cancer. 2008;60(6):744-56.
    PMID: 19005974
    DOI: 10.1080/01635580802192866
Matti Narkia

Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human... - 0 views

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    Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells.
    Habbel P, Weylandt KH, Lichopoj K, Nowak J, Purschke M, Wang JD, He CW, Baumgart DC, Kang JX.
    World J Gastroenterol. 2009 Mar 7;15(9):1079-84.
    PMID: 19266600
Matti Narkia

Arginine and cancer. - J Nutr. 2004 Oct - 0 views

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    Arginine and cancer.
    Lind DS.
    J Nutr. 2004 Oct;134(10 Suppl):2837S-2841S; discussion 2853S. Review.
    PMID: 15465796
Matti Narkia

Apoptotic effects of dietary and synthetic sphingolipids in androgen-independent (PC-3)... - 0 views

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    Apoptotic effects of dietary and synthetic sphingolipids in androgen-independent (PC-3) prostate cancer cells.
    Kent KD, Clubbs EA, Harper WJ, Bomser JA.
    Lipids. 2008 Feb;43(2):143-9. Epub 2008 Jan 10.
    PMID: 18188632
    DOI: 10.1007/s11745-007-3148-
Matti Narkia

White button mushroom (Agaricus bisporus) exhibits...[Nutr Cancer. 2008] - PubMed Result - 0 views

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    White button mushroom (Agaricus bisporus) exhibits antiproliferative and proapoptotic properties and inhibits prostate tumor growth in athymic mice.
    Adams LS, Phung S, Wu X, Ki L, Chen S.
    Nutr Cancer. 2008;60(6):744-56.
    PMID: 19005974
Matti Narkia

Vitamin C Antagonizes the Cytotoxic Effects of Antineoplastic Drugs - 0 views

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    Vitamin C antagonizes the cytotoxic effects of antineoplastic drugs.\nHeaney ML, Gardner JR, Karasavvas N, Golde DW, Scheinberg DA, Smith EA, O'Connor OA.\nCancer Res. 2008 Oct 1;68(19):8031-8.\nPMID: 18829561
Matti Narkia

A Mitochondria-K+ Channel Axis Is Suppressed in Cancer and Its Normalization Promotes A... - 0 views

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    A mitochondria-K+ channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth.
    Bonnet S, Archer SL, Allalunis-Turner J, Haromy A, Beaulieu C, Thompson R, Lee CT, Lopaschuk GD, Puttagunta L, Bonnet S, Harry G, Hashimoto K, Porter CJ, Andrade MA, Thebaud B, Michelakis ED.
    Cancer Cell. 2007 Jan;11(1):37-51.
    PMID: 17222789
    doi:10.1016/j.ccr.2006.10.020
Matti Narkia

Dichloroacetate induces apoptosis in endometrial cancer cells. - ScienceDirect - Gyneco... - 0 views

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    Dichloroacetate induces apoptosis in endometrial cancer cells.
    Wong JY, Huggins GS, Debidda M, Munshi NC, De Vivo I.
    Gynecol Oncol. 2008 Jun;109(3):394-402. Epub 2008 Apr 18.
    PMID: 18423823
    doi:10.1016/j.ygyno.2008.01.038
Matti Narkia

Dichloroacetate (DCA) as a potential metabolic-targeting therapy for cancer - British J... - 0 views

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    Dichloroacetate (DCA) as a potential metabolic-targeting therapy for cancer.\nMichelakis ED, Webster L, Mackey JR.\nBr J Cancer. 2008 Oct 7;99(7):989-94. Epub 2008 Sep 2. Review.\nPMID: 18766181 \ndoi:10.1038/sj.bjc.6604554 \n
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