The Countstar S2 System combines the two fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and Cell transfection efficiency through the use of preconfigured biological applications (BioApps). They are specifically optimized for analysis of primary cells from peripheral blood, including PBMC, CAR-T, NK cell and MSC, which commonly used in cell therapy.
The Countstar S3 System combines the three fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and T/NK cell mediated cytotoxicity through the use of preconfigured biological applications (BioApps). They are designed to simplify routine cell lab tasks，which leaves you time to focus on the research!
Peripheral blood mononuclear cells (PBMCs) are often processed to separate from whole blood by density gradient centrifugation. Those cells are consist of lymphocytes (T cells, B cells, NK cells) and monocytes, commonly used in the field of immunology, cell therapy, infectious disease and vaccine development. Monitoring and analyzing viability and concentration of PBMC is crucial for the clinical laboratories, basic medical science research and immune cell production.
The cytotoxicity % is calculated by the equation below.
Cytotoxicity % = (Live counts of control - Live counts of treated) / Live counts of control * 100
By labeling the target tumor cells with non-toxic, non-radioactive calcein AM or transfect with GFP, we can monitor the killing of the tumor cells by CAR-T cells. While live target cancer cells will be labeled by a green calcein AM or GFP, the dead cells cannot retain the green dye. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with membrane bound calcein AM, PI is used for stain the dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.
The Countstar® Rigel System combines three fluorescence channels with a bright field digital microscope, image cytometer, and cell counter into a single bench-top instrument. This application-driven, compact, and automated cell imaging system provides an all-in-one solution for cell counting, cell viability and T/NK cell mediated cytotoxicity using Countstar® BioApps.
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