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The cytotoxicity % is calculated by the equation below. Cytotoxicity % = (Live counts of control - Live counts of treated) / Live counts of control * 100 By labeling the target tumor cells with non-toxic, non-radioactive calcein AM or transfect with GFP, we can monitor the killing of the tumor cells by CAR-T cells. While live target cancer cells will be labeled by a green calcein AM or GFP, the dead cells cannot retain the green dye. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with membrane bound calcein AM, PI is used for stain the dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.

The Countstar S3 System combines the three fluorescence channels plus a bright field digital microscope, an image cytometer, and a cell counter in a single bench-top instrument. This application-driven, compact, automated cell imaging system provides an all-in-one solution for cell counting, cell viability and T/NK cell mediated cytotoxicity through the use of preconfigured biological applications (BioApps). They are designed to simplify routine cell lab tasks，which leaves you time to focus on the research!

The Countstar® Rigel System combines three fluorescence channels with a bright field digital microscope, image cytometer, and cell counter into a single bench-top instrument. This application-driven, compact, and automated cell imaging system provides an all-in-one solution for cell counting, cell viability and T/NK cell mediated cytotoxicity using Countstar® BioApps.