Group items tagged

In the biomedical field, the transfer of plasmid vectors into mammalian cells using conventional transfection is the most widely used method. Although a number of more advanced gene introduction vector systems have been developed in recent years (e.g., lentiviral vectors, adenoviral vectors, AAV vectors, and piggyBac, etc.), conventional plasmid gene expression vectors are still used in the majority of laboratories, mainly due to their technical simplicity and efficient transfection rates in various types of cells.

MicroRNA(miRNA) is a class of endogenous non-coding RNA found in eukaryotes with regulatory functions. They are about 20~25 nt. miRNAs are evolutionally-conserved and have a rich variety of species, they are not translated into proteins, but play an important role in regulating gene expression. miRNA can specifically bind to complementary regions within messenger RNA(mRNA) to promote its degradation or inhibit translation. miRNA is widely involved in cell proliferation, differentiation, apoptosis and cell cycle regulation, and plays a key role in gene experiments.

Lentivirus vector is a gene therapy vector developed on the base of HIV-1 (human immunodeficiency virus type 1). Lentivirus vector is a powerful tool for introduction of exogenous genes, with the advantages of wide infection spectrum, effective infection and long-term stable expression of exogenous genes. Now the lentivirus system has been widely used in various cell lines for gene overexpression, RNA interference, microRNA research, as well as in vivo experiments.

Seamless cloning technique from HANBIO is a new, fast and simple cloning method, which can insert one or more target DNA fragments at any site of the plasmid without any restriction enzymes and ligases. Breaking through the traditional double enzyme digestion and linking, the recombinant vector of high efficiency cloning can be obtained by one-step recombinant method, which greatly improves the working efficiency.

Non-coding RNAs (ncRNAs) are functional RNA molecules that cannot be translated into proteins. Common regulatory ncRNAs include miRNA, lncRNA, circRNA, piRNA, and so on. There are many ways for non-coding RNAs to function. They can interact with proteins, DNA and RNA, and participate in various cellular activities. Increasing experimental datas proved that they play regulatory roles in many biological processes such as gene silencing, cell differentiation, protein synthesis and functional regulation, metabolic regulation, organ development, etc.