Previous calicivirus reverse-genetics systems have relied on the transfection of either in vitro-transcribed, 5′-capped calicivirus genomic RNA (Chang et al., 2005; Sosnovtsev & Green, 1995) or cDNA constructs, followed by delivery of T7 RNA polymerase using a VACV recombinant (Sosnovtsev et al., 2002; Thumfart & Meyers, 2002). A recent report on rabbit hemorrhagic disease virus (RHDV) has also demonstrated that in vitro-transcribed, uncapped RNA is infectious when transfected into cells or delivered directly to the liver in vivo (Liu et al., 2006). Attempts to recover MNV by using any of these established approaches failed to produce infectious virus (data not shown).
CIDRAP - 1 views
COMMENTARY: Health workers need optimal respiratory protection for Ebola | CIDRAP - 0 views
Recovery of genetically defined murine norovirus in tissue culture by using a fowlpox v... - 1 views
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This paper contains very good background on the development of the MNV reverse genetics system. The norovirus genome is uncappped, but transfection with uncapped mRNAs does NOT produce many progeny virions.
Ribosomal frameshifting on viral RNAs - 0 views
Farmers Gain Weapon Against Devastating Pig Virus - NYTimes.com - 0 views
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“I’ve been in this industry for 37 years, and I have never seen it pull together like it has in this crisis,” said Bill Marks, chief executive of MJ Biologics in Mankato, Minn., which is hoping to get the Agriculture Department’s approval soon for the third vaccine.
ViralZone: Ebolavirus cycle - 0 views
ViralZone: Ebolavirus - 0 views
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Infection Mechanism of Genus Ebolavirus - MicrobeWiki - 0 views
PLOS Pathogens: Antigenic Subversion: A Novel Mechanism of Host Immune Evasion by Ebola... - 0 views
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