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CIDRAP - 1 views

www.cidrap.umn.edu
shared by Casey Finnerty on 23 Sep 14 - Cached
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Recovery of genetically defined murine norovirus in tissue culture by using a fowlpox v... - 1 views

vir.sgmjournals.org/...2091.full norovirus
shared by Casey Finnerty on 22 Sep 14 - No Cached
  • Previous calicivirus reverse-genetics systems have relied on the transfection of either in vitro-transcribed, 5′-capped calicivirus genomic RNA (Chang et al., 2005; Sosnovtsev & Green, 1995) or cDNA constructs, followed by delivery of T7 RNA polymerase using a VACV recombinant (Sosnovtsev et al., 2002; Thumfart & Meyers, 2002). A recent report on rabbit hemorrhagic disease virus (RHDV) has also demonstrated that in vitro-transcribed, uncapped RNA is infectious when transfected into cells or delivered directly to the liver in vivo (Liu et al., 2006). Attempts to recover MNV by using any of these established approaches failed to produce infectious virus (data not shown).
  • Casey Finnerty on 22 Sep 14
     
    This paper contains very good background on the development of the MNV reverse genetics system. The norovirus genome is uncappped, but transfection with uncapped mRNAs does NOT produce many progeny virions.
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Prevent the Spread of Norovirus | Features | CDC - 1 views

www.cdc.gov/norovirus norovirus
shared by Casey Finnerty on 22 Sep 14 - No Cached
    • Casey Finnerty
      Casey Finnerty on 22 Sep 14
       
      So happy, so innocent. Little do they know what awaits them. (Don't you love stock photos?)
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Identification of Protein Interacting Partners Using Tandem Affinity Purification - 0 views

www.ncbi.nlm.nih.gov/...PMC3399501 norovirus
shared by Casey Finnerty on 19 Sep 14 - No Cached
  • Casey Finnerty on 19 Sep 14
     
    This paper includes a video that explains the use of Tandem Affinity Purification by the Goodfellow group.
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American Society for Microbiology:Norovirus Disinfection: How Much is Enough? - 0 views

www.asm.org/...isinfection-how-much-is-enough microbiology disinfection
shared by Casey Finnerty on 19 Sep 14 - Cached
  • wipe with a wet cloth, and then disinfect with chlorine.
  • Household bleach, says Duizer, is an average about 5 percent chlorine when new (concentration declines with age), or 50,000 PPM, and thus, can be diluted 50-fold to achieve the 1,000 ppm.
  • Norovirus is apparently no more resistant to cleaning and disinfection than other pathogens, says Duizer. The virus’ efficiency in causing outbreaks “is more likely due to their extremely low infectious dose
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