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katherine-medina

Dandelion root extract affects colorectal cancer proliferation and survival through the... - 0 views

www.ncbi.nlm.nih.gov/...PMC5341965

Dandelions Cancer-fighting HSR-2023 potential research idea botany colon-cancer

shared by katherine-medina on 09 Jun 23 - No Cached
Sean Nash liked it
  • of an aqueous dandelion root extract
  • caspase-8 activation was not essential for the induction of cell death in colon cancer cells as an inhibition of caspase-8 activation did not alter the cytotoxicity of DRE
  • We have been able to identify four pharmacologically active components, α-amyrin, β-amyrin, lupeol and taraxasterol, in two out of the six bioactive fractions, but the anti-cancer activities of the individual compounds were not as strong as that of the unfractionated DRE indicating, clearly, the benefits of using the whole extract.
  • ...16 more annotations...
  • which might represent a novel non-toxic alternative to conventional cancer therapy available today.
  • These results clearly indicate that dandelion root extract can inhibit the ability of colorectal cancer cells to migrate and invade, and therefore metastasize to secondary locations.
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      Wow. I like to see that in 3 different studies DRE was proven to selectively pick the cancer cells, and ignore the normal cells.
  • morphological differences in tissue slices between the control untreated and the DRE treated group
  • aken together, these results established that systemic oral intake of the DRE was safe and its anti-cancer efficacy should be further investigated.
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      I love the fact that they yet again state that I should look more into this topic.
  • , but the DRE treatment efficiently suppressed the growth of both p53 WT and p53 mutant tumors in-vivo (Figure 4B – 4C)
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      great, they suppressed the growth of the tumors.
  • with no difference between the control and DRE treated samples of NCM460
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      I would not have thought that the mitochondria would be left alone by the drug.
  • We observed a decrease in the viability of cells treated with α-amyrin, with 10 μM as the most effective concentration.
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      Hmm. the beginnings of narrowing down what it is about the plant that is able to fight cancer.
  • The results showed a progressive destabilization of the mitochondrial membrane following the DRE treatment, which was observed as early as 30 minutes post treatment (Figure ​6C). Pro-caspase-8 (green) was localized in the mitochondria (red) in control untreated cells; however, following the DRE treatment, activated caspase-8 was released from the mitochondria into cytoplasmic space, as indicated by the dispersed green fluorescence (Figure ​6C
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      Pro-capase-8 helps to fight against the cancer
  • suggesting that in HT-29 colorectal cancer cells the DRE-induced cell death was caspase-8 independent.
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      So essentially caspase 8 had nothing to do with it
  • Others suggest that following activation, caspases re-localize to the mitochondria, where they interact with other pro-apoptotic proteins during the progression of apoptosis [15]. A third option, put forward by Qin and colleagues, suggests that inactive caspases are kept in the mitochondria, but following apoptotic stimuli and activation, they are released from the mitochondria into the cytoplasmic peri-nuclear space [
  • However, these results indicate that DRE and its anti-cancer components must be absorbed and circulated, in order to reach the site of the tumor (in order to inhibit tumor growth).
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      So it needs to be drank, or swallowed in a pill form to work.
  • , we confirmed the vulnerability of cancer cell mitochondria by showing that the DRE treatment led to a decrease in the mitochondrial membrane potential and increase in ROS levels in the isolated mitochondria.
  • caspase-8 specific inhibitor, IETD-fmk, did not change the DRE response in these cells. This was in contrast to our previous study in leukemia and pancreatic cancer cells
    • katherine-medina
      katherine-medina on 09 Jun 23
       
      For each different cancer a new slightly different result is produced
  • he pro-apoptotic genes including Caspase-1, Interferon gamma and the TNF ligands and receptors, were up-regulated in HT-29 cells, prior to the apoptosis induction, while the same genes were down-regulated in NCM460 cells.
  • Previous findings show that taraxasterol has anti-inflammatory and chemopreventive activit
  • suggesting its importance in the anti-cancer activity of dandelion root extract, especially on the expression levels of COX-2. Additionally, we show that 10 μM lupeol is not very effective on its own
  • katherine-medina on 09 Jun 23
     
    Yet another article about how DRE can fight against cancer.
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