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Gene synthesis does not require a template and involves amplifying DNA fragments in vitro using overlapping short primers, which range in length from 100bp to 10kb. This method is suitable for obtaining various target genes that are difficult to access, non-natural sequences, amino acid mutations, or rare codon substitutions, allowing for the fastest acquisition of the desired sequence with 100% accuracy.

Luciferase is a general term for enzymes that can produce bioluminescence in nature, with the most representative ones are F-Luciferase from fireflies and R-Luciferase from renilla. Under the catalysis of luciferase, luciferin can efficiently convert to oxidized luciferin and emit strong fluorescence at the same time. Therefore, researchers used luciferase to develop a highly sensitive and convenient gene reporter system. Currently, this system is widely applied in various fields, including transcription factor binding site and promoter activity analysis, RNA degradation, signal transduction, drug screening, and in vivo animal imaging.

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A monoclonal CRISPR/Cas9 knockout cell line is a cell line that has been genetically modified using the CRISPR/Cas9 gene editing technology to create a specific knockout of a target gene. This cell line contains a single, identical copy of the modified gene, making it a monoclonal cell line. The knockout of the target gene allows researchers to study the function of that gene in the cell and its role in various cellular processes. These cell lines are valuable tools for studying gene function, drug discovery, and disease modeling.

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