And tnf soluble receptors in pulmonary sarcoidosis


Tnf receptors(Tnfr), which are known to inhibit TNF activity, were recently described in the lungs of subjects

To investigate for the first time the relationship between soluble receptors and tnf bioactivity in the lungs of subjectssarcoidosis is a multisystem disorder with 90% of patients exhibiting some degree of pulmonary involvement.1Pulmonary sarcoidosis is characterised by an accumulation of T lymphocytes in the alveolar spaces, preceding the development

Of noncaseating epithelioid granulomas which can lead to interstitial fibrosis in some cases.2 The clinical syndrome can be staged according to the appearance on the chest radiograph.Stage i disease is defined by the

Presence of bilateral hilar lymphadenopathy(Bhl)With no interstitial changes, whereas stage ii disease denotes bhl in association

With beats by dre uk wireless nodular shadowing.Stage iii is a more generalised shadowing in the absence of bhl, and stage iv disease classification

Requires radiographic evidence of fibrosis.Stage i sarcoidosis is usually a selflimiting disease, whereas stages iiiv may

Be associated with a more progressive clinical course.

Tumour necrosis factor alpha(Tnf)Is a pleiotropic proinflammatory cytokine which has been implicated in the pathogenesis

Of a number of inflammatory lung diseases3

4 by virtue of its ability to increase the respiratory burst in neutrophils5 and induce pulmonary vascular permeability, 6 thus promoting inflammatory cell accumulation.Sarcoidosis has been described as an example of a disordered inflammatory

Response with increased production of a number of proinflammatory cytokines, 711 but cheap beats by dre solo hd the role of f in the pathogenesis of sarcoidosis remains uncertain.The documented production of interferon and

Cholecalciferol in patients with sarcoidosis, both of which are extremely effective priming agents for tnf, suggests the

Potential for an active role if it is produced in this condition.12 Several studies have reported increased levels of TNF in patients with sarcoidosis, 1315 although this has not been a consistent finding.16 Indeed, many of the clinical and pathological features associated with TNF such as systemic disturbance, weight loss, and

Tissue necrosis are not commonly found in caucasian subjects.This led to the hypothesis that tnf activity might be inhibited

In sarcoidosis.In 1990 an inhibitory factor was described, but not characterised, in the circulation but not in the lungs

Of patients 17 since then two forms of soluble tnfReceptors(Tnfr)Have been characterised from human urine18the p55(Cd120a)And the p75(Cd120b)Receptors.Both are identical to the membrane bound form and are cleaved enzymatically

From the cell surface by a metalloproteinase enzyme.19 The p55 receptor is the main signalling receptor and is ubiquitous in human tissue, whereas p75 chpbtsbydreuk receptor expression appears

To be restricted to cells of the myeloid lineage.20 Both TNFRs have been found to inhibit the bioactivity of TNF in vitro, 21 although there is a report suggesting that binding of TNF to its soluble receptors stabilises the trimeric structure of

Tnf and therefore may prolong its bioactivity in vivo.22 It has become increasingly apparent that cytokines do not work in isolation but function as part of a tightly regulated cytokine

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Cub scout tristan gee from bromsgrove worcestershire bags all 54 badges in just two years


A 10yearold boy was today hailed britain's most dedicated cub scout after earning all 54 badges in just two years.

Tristan gee has achieved one new badge every two weeks since he joined his troop in 2010 and has so many he almost ran out of space on his cub jumper.

The schoolboy joined just a handful of other christian louboutin chaussures soldes cubs in britain to complete every badge and was awarded his last one map reading on his final ever meeting before joining the scouts this week.

He first joined romsley cub scouts, in his home town of bromsgrove, worcestershire, when he was eight and set himself the challenge of earning every badge. home

He had to work almost every school night and weekend to achieve his goal.

On sunday, tristan was presented with a special neckerchief and the chief scout's silver award the highest award available to cubs in honour of his remarkable achievements.

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The details of blake lively and ryan reynolds' wedding


Surprise!Ryan reynolds and blake lively have tied the knot in a secret wedding ceremony.

The pair, who have been together for a year, reportedly married over the weekend, a source told us magazine people.

While representatives for both stars had no comment, the source said the ceremony took place on sunday evening at the boone hall plantation in mt.Pleasant, south carolina, one of america's oldest working plantations and where the romantic movie the notebook was filmed.

The reception was held in a white tent set iaelorsoutlethaburg up at the plantation.

"Ryan and blake wanted the event to look and feel like home,"Wedding planner tara guerard told e!News.

"With decor and live michael kors deutschland music, we all created the charm, elegance and magic of times passed. "

Blake wore a custom marchesa with"A handdraped silk tulle bodice adorned with crystal and rose gold embroidery".

Her bridesmaids also wore custom marchesa gowns and all wore custom christian louboutin shoes.

Wedding plans had been underway since june when tara accompanied the pair on a trip to look at venues.

The gossip girl star's mother and sister were spotted in town in the lead up to the event, and a source confirmed that blake, 25, and ryan, 35, had dined in nearby charlestown with bette midler earlier in the weekend.

While the wedding was kept top secret, it has emerged that the cake was driven down to the venue from virginia, and that blake's good friend florence welch, from florence and the machine, performed three songs.

The couple, who costarred in the green lantern, have kept a relatively low profile since purchasing a home together in new york earlier this year.

This is blake's first marriage.Ryan was previously married to actress scarlett johansson, but the two divorced in 2010 after two years of marriage.

Thank you for sharing your opinions with other users of

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Ashton kutcher meets mila kunis' parentswhile his divorce proceedings with estranged wife demi moore seem to be moving at a snail's pace, ashton kutcher's relationship with girlfriend mila kunis is picking up speed.

Royal wedding joy:Beatrice is engaged!The parents are delighted now the push for a ceremony fit for a princess.

Natalie bassingthwaighte welcomes second childnatalie bassingthwaighte has welcomed her second child, a son named hendrix john hickson mcglinchey.

Bon jovi says his daughter's overdose was"Horrible"Rock star jon bon jovi has opened up, revealing his"Worst moment as a father"Was the day his 19yearold daughter overdosed on heroin in november last year

Michelle bridges stole my husbandthey may be the most talked about new couple in the country, but a shocking secret lies at the heart of the biggest loser's redcarpet romance.

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And effective therapy for inflammatory bowel disease


August 25, 2003(Bethesda, md)Inflammatory bowel disease(Ibd)Refers to crohn's disease and ulcerative colitis, debilitating illnesses characterized by chronic recurrent ulceration of the bowel, abdominal pain, digestive problems, diarrhea or constipation.The national institutes of health estimates that some two million americans suffer from visitthisuser'swebsite this disorder, which is of unknown origin, but likely caused by a combination of genetic, environmental, and immunologic factors.

Medical researchers have made considerable efforts to establish a genetic linkage between crohn's disease and the nod2 protein associated with programmed cell death and activation of nf b, a transcription factor involved in the production of cytokines and chemokines necessary for inflammation.

Regulation of nf b function has been documented by several agents used in the management of ibd, such as corticosteroids, sulfasalazine, and 5aminosalicylates(5ASA).Furthermore, antisense oligonucleotides directed against the p65 subunit(A polypeptide contributing to the activation of nf b)Have been shown to diminish disease activity in an animal model of colitis.Recent work has shown that dietary constituents such as curcumin may also potently inhibit nf b and diminish attenuate proinflammatory molecule expression.Curcumin is a component of the spice turmeric(Curcuma longa)Used in curries and mustard, whose antiinflammatory properties have been recognized for years.These effects are related, in part, to inhibition of the activities of the cyclooxygenase, lipoxygenase, and nf b beats by dre pro sale in several cell systems.Furthermore, its role in the attenuation of colonic cancer in animal models has also been established.

Management of ibd involves the use of 5asa and immunosuppressives such as corticosteroids and 6mercaptopurine as well as its precursor azathioprine.Novel agents such as monoclonal antibodies against tnf have been developed and demonstrate clinical efficacy.However, these agents are expensive and not without side effects.Consequently, there is a need for alternative agents that may be equally or more effective as well as being cheaper.Both curcumin and sulfasalazine target ikk molecules;The importance of inhibition of i?B kinase complex by curcumin has never been tested in ibd.Here researchers show that this compound has beneficial effects in a rat model of ibd, opening the door to possible future human studies.

A new study http://www.teamskyuser.co.uk/

The new study now provides the first evaluation of curcumin and its effects on nf b in an experimental model of ibd.Salh, k.Assi, k.Parhar, d.Owen, and a.Gmezmuoz, at the jack bell research centre at the vancouver general hospital, and v.Templeman and k.Jacobson of the department of pediatric gastroenterology, children and women's hospital, all in vancouver, british columbia, canada.Their findings appear in the july 2003 edition of the american journal of physiologygastrointestinal and liver physiology.Sevenweekold c3h mice had inflammation induced with instillation of 100 l of dnb(60 mg/ml)In 50% ethanol with control animals receiving 100 l of 50% ethanol alone.After this, they were kept in position for 30 seconds before being returned to their cages.On day 5, postinduction was conducted for evaluation of the colitis.Test groups of five mice had curcumin added to their diet at a concentration of 0.25% beginning five days before the DNB instillation.

Several parameters were determined in the inflamed mucosa.Animals were weighed daily after induction of colitis and the data shown are representative of all experiments.There was a clear reduction in the amount of weight loss in the animals pretreated with curcumin.

Curcumin improves intestinal cell function in dnbinduced colitis.After treatment of animals, colons were harvested and histological evaluation was carried out after staining with hematoxylin and eosin.There was mucosal ulceration, thickening of the wall, and significant infiltration with inflammatory cells in the dnbtreated animals that is reduced in those animals pretreated with curcumin.

When animals were pretreated with curcumin, there was a clear reduction in dna binding, thus verifying that curcumin does indeed inhibit nf b activation in the colon in vivo.These findings indicate for the first time that curcumin is able to impact on an important transcriptional mechanism in the gastrointestinal tract.In addition to the demonstration that it is able to diminish inflammatory activity in ibd, the canadian team demonstrated it can reduce nf b dna binding activity as well as inhibiting the activation of p38 mapk antibodies and that the antiinflammatory effects of curcumin involve a reduction in myeloperoxidase activity, a reduction in the number of infiltrating neutrophils, as well as a reduced expression of the message for il1.

Precisely how curcumin achieves its effects is not clear.It has been shown to possess free radical scavenging(Antioxidant)Properties in addition to its known effects on the activation of nf b.Its in vivo effects may well rely on a complementation of these two and other activities.

Although curcumin has been shown to be safe up to levels as high as 10 percent(100, 000 ppm), the researchers showed effectiveness at a concentration as low as 0.25 percent.This dose was well tolerated with no reduction in dietary intake.Further work will help to clarify the optimal dose for this and other models of ibd.

This research proves that curcumin may prove to be a cheap, welltolerated, and effective therapy for inflammatory bowel disease.This food ingredient has for generations been regarded as a potent antiinflammatory within many eastern civilizations.It is equally intriguing that the same agent is a potent antineoplastic agent.It may hold promise for the treatment of ibd in humans.

Source:July 2003 edition of the american journal of physiologygastrointestinal and liver physiology.

The american physiological society(Aps)Was founded in 1887 to foster basic and applied science, much of it relating to human health.The bethesda, mdbased society has more than 10, 000 members and publishes 3, 800 articles in its 14 peerreviewed journals every year.

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County trashes garbage bags with mlk's face


Martin luther king jr.'s profile is on voter ballot envelopes, on metro buses, at the county council chambers, and prominently displayed on the county's Web site.

The bags were quietly pulled from use earlier this summer, even though no one from the public had complained.The preemptive move opened a window into the delicate decisions officials have made after king county adopted the face of the revered figure as its official emblem.

"It's new territory,"Said christian louboutin france carolyn duncan, the county's communications director.

King county the mostpopulated county in washington, with a county seat of seattle, and the 14th largest by population in the nation adopted the logo in 2006 and unveiled it the next year.It features a striking profile of king in a black and white silhouette.Vice president william rufus devane king, a slave owner.

The possibility of plastering king's face on mundane county items like trash bins or prisoner uniforms raised eyebrows among county officials when the proposal was approved.The old logo was a generic crown.

The decision of where the new logo goes lies with duncan and king county executive ron sims, who is black.As the head of communications, duncan is in charge of the messages symbolic or not king county sends.

Her challenge:To maintain the county brand, without splashing king's face on something that's demeaning to king's history.

"Some of the things that people were concerned about were if the logo was on the floor, if the logo was on garbage cans or dumpsters,"Duncan said. "It seemed unseemly to have an icon like dr.King, to have his image on something like a waste bin at a park. "

Duncan said that the old crown logo was not used on trash bins or prison uniforms, but communication directors in county departments were directed to look carefully at where the logo is used.

Some county officials and africanamerican leaders in seattle have no qualms about using the image of king.

"Personally, we should use the logo wherever we traditionally printed that logo,"Said larry gossett, the county council member who led the name and logo change.Gossett said the symbol can be used"To do better. "

Gossett, a longtime leader in the africanamerican community here, knew from the beginning there would be challenges.His own son told him that he didn't want to see king's face on a police car.

Tye heckler of heckler associates a design and advertising firm that helped developed the starbucks brand said once the county approved the ristianlbtinsoldes logo, it accepted all the risks of using king's face, including the possibility that the logo may be defaced someday.But as a government, heckler said, they have the freedom not to use the logo.

Steve kline, a spokesman at the king center in atlanta, said there are no set guidelines when it comes to the use of king's image, but there have been examples of inappropriate use.

"Many years ago, some guy tried to make a knife with mr.King on it,"Kline said. "Stuff like that is always a concern.They take it of a casebycase basis.They do try to monitor the commercial use of it. "

While kline said that king county is believed to be the first government to take king's likeness as a logo, scores of local governments have named streets, parks and even pool centers after the civil rights leader.But many of those moves have carried a stigma:Many martin luther king jr.Streets, avenues, ways and boulevards are in lowincome, crimeridden areas.

"You can use it to inspire and move for reforms(King)Could have been be very proud of,"He said.

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Swimsuit model proves she can toss a football in pumps video


Kate upton is not afraid to play with the boys in 5 1/2 inch heels, no less.The swimsuit model stopped by fox and worked on her football throws with jason pierrepaul of the new york giants.And we must say, she has a pretty good arm.

Though upton didn't land any of her passes in the tiny hole in the net, she did iaelorstasengünstig succeed in showing off her athletic abilities in a white tweed jacket, black skinny jeans and christian louboutin pumps.

You've got to give it to her.The sports illustrated model showed that she doesn't just have the looks, but can actually toss a football around with a fox michael kors taschen günstig sportscaster and a giants defensive end.

Maybe she's been practicing her spiral with jets quarterback mark sanchez.

Watch the clip above to see kate's huge heels and don't miss the creepy sportscaster you'll know the one.

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An herbal extract inhibits the development of pancreatic cancer


(Philadelphia)An herb recently found to kill pancreatic cancer cells also appears to inhibit development of pancreatic cancer as a result of its antiinflammatory properties, according to researchers from the kimmel cancer center at jefferson.The data were presented at the aacr 100th annual meeting 2009 in denver. beats by dre sale online

Nigella sativa seeds and oil are used in traditional medicine by many middle eastern and asian countries.It helps treat a broad array of diseases, including some immune and inflammatory disorders, dr.Arafat said.Thymoquinone almost completely abolished the expression of several inflammatory cytokines, including tnfalpha, interleukin1beta, interleukin8, cox2 and mcp1, an effect that was more superior to the effect of trichostatin a.

The herb also inhibited the activation and synthesis of nfkappab, a transcription factor that has been implicated in inflammationassociated cancer.Activation of nfkappab has been observed in pancreatic cancer and may be a factor in pancreatic cancer's resistance to chemotherapeutic agents.When animal models of pancreatic cancer were treated with thymoquinone, 67 percent of the tumors were significantly shrunken, and the levels of proinflammatory cytokines in the tumors were significantly reduced.

Inflammation has been implicated in the beats by dre outlet development of several solid tumor malignancies.Chronic pancreatitis, both hereditary and sporadic, is associated with the risk of developing pancreatic cancer.

"These are very exciting and novel results,"Dr.Arafat said. "Not only patients with chronic pancreatitis could benefit from this, but also several other groups with risk of development or recurrence of pancreatic cancer, such as highrisk family members and postsurgical patients.These potent effects show promise for the herb as a potential preventive and therapeutic strategy for pancreatic cancer.More importantly, the herb and oil are safe when used btsbydreuale moderately, and have been used for thousands of years without reported toxic effects. "Pancreatic cancer is the fourth leading cause of cancer death in the united states, with approximately 32, 000 deaths a year.Only five percent of individuals with pancreatic cancer live for at least one year after diagnosis.

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Corporation plans to abolish plastic bags


The madurai corporation is planning a complete ban on plastic cups and carry bags in the city.This issue is likely to be taken up for discussion at the council meeting to be held on friday.

Based on a report prepared by an expert team, the corporation is mulling the ban, and a final decision will be taken based on the response from the councillors.

Official sources told the hindu on thursday that the civic body aim was to make madurai totally free of plastic cups and bags, irrespective of their thickness.

There is a ban on the manufacture and use of plastic bags which have a thickness below 40 microns.But, the madurai corporation may try to impose a total ban on the use of plastic carry bags and plastic cups irrespective of their thickness, sources said.

The move to ban them was initiated because they were found to be a major cause for blockages along drainage lines and water channels.

Plastic cups and carry bags get dumped everywhere, the drainage system is getting affected.It is leading to mosquito breeding and also the soil loses fertility, according to an official.

The corporation will initiate steps in such a way that paper cups and paper bags will gradually replace the ones made of plastic in the interest of the people.

As and when the guidelines are ready and if it gets approval from all the stakeholders, special inspection teams would be deputed to conduct raids on shops and business establishments and seize louboutin pas cher the banned plastic items, if any.

Meanwhile, in another major step, the corporation schools in madurai have gone solar to meet their power requirements.

With the one kilowatt solar power generated, the corporation schools will be able to use lights and home fans in a few classrooms.

The mayor and commissioner have announced that other corporation schools will also be provided with solar power units when schools reopen after vacation.

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Most of ladies cannot refuse the temptation of beautiful shoes and once they fall in love with a pair of shoes, they try their best to get them no matter how much cost they should pay, for they cannot imagine what their lives would be without the pair of shoes in their shoe cabinets, even though they have to use up the clothing budget of the next season advanced or they have to delay the date when they repay their credits in order to own such shoes.

For ladies, no matter how many pairs of shoes they have, they would never consider that is enough.Even, ladies start to buy shoes for collections rather than for wearing.There is a shoes designer, named christian louboutin, who designs shoes with red soles for ladies, was spoken highly by ladies and become popular in the fashion shoes world no matter how shoes he designs change in styles.More magically, his shoes even can help his customers find their love.Thank you very much!You know what, my husband fell in love with just because he thought i was sexy when he met me the first time when i was wearing a pair of your shoes, once one lady told him so.

Christian has his own understanding of ladies and from shoes he designed we can concluded that he believes perfect ladies in his mind should be mysterious and sexy.All shoes designed by him have heels as high as 12 cm which can always make ladies look sexy and attractive even they just were observed throng their backs.

Such shoes are admired by so many people especially by those hollywood female movie stars and almost ever hollywood movie star has several pairs of christian louboutin shoes to wear when she has to attend some occasions.

Shoes designed by famous designer make people feel exciting iaelorshandtasengünstig and surprise not only by their special and perfect design styles, but also by the high prices.Most christian louboutin shoes are expensive and for those people who have no so much money, they are luxuries.But if you really want to own a pair, you can try to find stores where discount prices of this brand are available and if you are lucky enough, perhaps you can buy what you desire at a quite reasonable and satisfying price.

1.It is small, easy so that you can is to associated with focus that during a provide belly and that somers grill.The easytoopen top is guaranteed cracked c.An appropriate model is required to be selected for each brand to boost the comparison process.Work diligently and professionally, they have done this for many years having achieved so much for you.Hence, the gloves, jerseys and shorts are very much in demand.The fighter can be assured about the safety of his body during fights.With meticulo.And why not!Without them you dont have a company.It is corporate gifts and awards time.All corporate companies have a varied and expansiv.

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Amgen announces positive study results of kineretr in btsbydrdreoutletuk rheumatoid arthritis disease progression and in the treatment of juvenile rheumatoid arthritis


Late breaking poster and press conference featuring new kineret

N.Annualexploring a biologic theRapy's impact on disease progression inchronic, progressive autoimmune disease causing connectivetissue inflammation, mostly in synovial joints.It can occur at any age, is more common in women, and has an unpredictable course. (Ra).Data from a study evaluating the ability of

KineRet(R) (Anakinra)To inhibit bone and joint damage in adult ra

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Kineret in patients with juvenile rheumatoid http://www.teamskyuser.co.uk/beats-by-solo-diamond.html arthritis juvenile rheumatoid arthritis

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Chronic inflammatory arthritis that begins in childhood, characterized by swelling, tenderness, and pain in one or more joints and by lymph node and splenic enlargement. (Jra jrajuvenile rheumatoid arthritis)Will be

Featured tomorrow at a press conference at the 66th american college of

Rheumatology annual in new orleans.

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Signal that we may have another beats by dre outlet uk therapeutic option for slowing the

Progression of ra,"Said dr.William shergy, a rheumatologist rheumatologist //(Roo?Mahtolahjist)A specialist in rheumatology.

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Consuela ristianlbtinpaser bags


Lol wow i've never heard of someone having so much hate over a purse.If u don't like them just don't get one simple as that right? .Ha or maybe the girl your man is with has a consuela handbag you're just hating?Who knows but whoever made this topic has to be an immature little girl!God bless you sweetie!

Wowww a big imagination in a little brain hahaha hate over a purse?Is called freedom of speech.I bet you are that kind of woman that"Bless"Everybody but can't quit spit the poison.

Obviously whoever started this topic is a snotty little b!Tch whom claims to be"Wealthy".Yet can't post their real name, she is simply a coward hiding behind a computer screen because if we really knew who this person was we'd be using our"Freedom of speech"To bash them too!How sad though that this person has to talk crap about something to make his/ herself feel better but i guess if that's what you want to do topix is the place to do it!But to me a purse is a purse is a purse is a purse is a purse is a purse is a purse.Get it!Obviously whoever started this topic is a snotty little b!Tch whom claims to be"Wealthy".Yet can't post their real name, she is simply a coward hiding behind a computer screen because if we really knew who this person was we'd be using our"Freedom of speech"To bash them too!How sad though that this person has to talk crap about something to make his/ herself feel better but i guess if that's what you want to do topix is the place to do it!But to me a purse is a purse is a purse is a purse is a purse is a purse christian louboutin chaussures pas cher is a purse.Get it!

Heyyy"Consumer"Give us your real name, be yourself an exemple or are you a chihuahua big mouth!Pndeja retarded.

What a b!Tch, i don't know w/ you're doing here go and do the only good think christian louboutin paris soldes you can do well, spread your legs hehehe

It's time to do something constructive about those worries that have been nagging away at you.It's tough to have to face up to them, but you need to make a new start.You can't allow them to hang over you much longer.You may also have to reveal something that's been a secret until now, and which will leave you feeling exposed or embarrassed when it finally comes to light.

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With all the things that one can think of in terms of what can adorn a lady and make her more beautiful, shoes have managed to find an important place in designer items.One of the shoes whose sales have shot michael kors outlet hamburg up to a great extent in recent times are christian louboutin replica shoes.If a lady loves to have a huge and beautiful collection of shoes but cannot afford to buy the original designer shoes, she can go for replica shoes.One can search through the many online stores that claim to sell original christian louboutin shoes to find that special pair.In reality, most of these stores sell copy shoes.That is why the shoes are offered at a far lesser price as compared to the ones that are available at the company's outlets, which makes even a pair of louboutin supra fifre boots easily within reach.These sites give huge discounts on the christian louboutin replica shoes and guarantee their money would be given back if they do not have a quality similar to the original.However, be assured that these shoes are manufactured in a way that it is not so easy to distinguish between the original and the replicas.If you have purchased a pair christian louboutin replica shoes, do not worry and think that your shoes are of bad quality.The replica shoes are also made with quality and the best materials.They have to match the original ones after all.These shoes are the ones that make one stand out with the latest fashion trends but with the lowest of investments.They are superbly manufactured that no one would differentiate whether you are wearing original or reproduction shoes.So, go ahead and search for the shoes to make your shoe collection more gorgeous.In case you have christian louboutin replica in your mind, expect the rates for the pieces to be more reasonably priced.People, though are skeptical of putting on a replica, so one must know the advantage that such items carry, especially when they are bought from a good replica store like replica handbags pro.There is the louboutin supra fifre boots, which has been one of the most desired product from the christian louboutin collection.However, it is very unAffordable for many women.In case you opt for the same from replica handbags pro in the form of christian louboutin replica shoes, it is possible that you could get the piece for less than 50 percent of the original price.Once more, the quality of the material and the elegance of the design is kept intact in spite of the lower price range thanks to the designers who have come out with the replica version.Without any doubt, fashion is in the blood of the modern day woman, but you should also be aware that one should not buy fashion at the cost of their entire savings.This is where christian louboutin replica products bought from replica handbags pro can help you in being ahead.Without compromising on quality, you can give a new meaning to the term"Affordable"In the designer world.Again, the options are not less with replicas.Some people actually admit of finding a greater variety in colors and sizes with replicas than from the originals.So, don't think twice, make it a point that you make a purchase of replica items of christian louboutin today.

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Alyeska resort to install new chair 6 drdrebtshdphones high


Construction and installation of the chairlift by doppelmayr usa inc.Is expected to occur from may 6 through october 31, 2013 at an estimated cost of $6 beats by dre outlet uk million.

Servicing high volumes of skiers and riders, the currently existing chair 6 provides primary lift access to the upper mountain trails at alyeska resort and is considered by many to be the heart and soul of alyeska ski resort.Popular skiing and snowboarding runs accessed by chair 6 include christmas chute and the north face, silvertip, trapline, mighty mite and high traverse.The existing chairlift was originally installed in 1988.

Am very excited for this new addition, said di hiibner, ski area general manager.Are committed to ensuring we have a reliable chairlift for our guests.With this new lift, alyeska resort will have invested over $14 million dollars in ski area improvements over the last two years.We are committed to continued improvement of the ski area for the enjoyment of our guests.Skiers and riders at alyeska will notice that the new chair 6 will be substantially identical to ted express, the highspeed quad installed last summer to replace the old chair 4.The project also includes installing a new weather station at the top, upgrading utilities and installing natural gas.The installation is scheduled to take five months.

Due to the installation of a new chair 6, there will be no may skiing this year.During the summer season certain areas of the mountain will be closed intermittently to hiking, biking and other traffic during the beats by dre uk wireless summer season.Closures will change on a daily basis and the resort will strive to have as much terrain open as often as possible.The resort will update its skisnow hotline(9077547669)And its website summer trails report on a regular basis and urges guests to check for construction updates before heading up the mountain to go hiking or biking.

Repairs of the damaged tram cabin, made by cwa constructions of switzerland, are nearly complete.Tram operations will cease on or about april 23, 2013, in order to affect final repairs.The tram is currently scheduled to reopen with two fully operational cabins on or about may 23, 2013.

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Common causes and Click to enter website what to do


When you look in the mirror and notice dark bags under eyes on your face then you would perhaps like to know what they are exactly and what is causing them to appear.Periorbital puffiness and periorbital dark circles are the medical terms for the two conditions that together cause dark bags under eyes.These are different conditions but often occur at the same time.If your parents had this skin condition then you most likely will too as genetics play a major part in how your skin ages.Skin as it gets older is less supple and is also thinner.

Some individuals are genetically predisposed to have a skin texture that is thinner than the norm around the eyes and can have the numerous veins which lie underneath the skin in this area showing through, making the skin to look darker.Interrupted sleep cycles are another common cause of dark bags under eyes.This can occur due to lack of sleep or insomnia and also because of excessive sleeping.Emotional stress or anxiety can lead to insomnia and dark bags under eyes so if this is the problem, relaxation methods must be tried.Occasionally, some internal health problem such as kidney and thyroid diseases could also cause dark bags under eyes.

Menstrual cycles and pregnancy can cause hormonal variations that make skin paler and dark veins prominent.Improper nutrition can deprive your body of the nutrients, minerals and vitamins that it needs to maintain skin healthy.Some types of medications may cause the the dilation of the blood vessels underneath the skin christian louboutin soldes layer and this often leads to darkening of eye bags because the skin layer in this area is very sensitive to changes in blood circulation.Contact with ultra violet rays from the sun can produce the chemical substance melanin which that causes pigmentation changes which could cause those dark bags under eyes.

If you have allergies to things like pets, foods or environmental components then your eyes could become irritated and itchy and this could cause dark bags under eyes.If ever the allergic reactions are of the hay fever type from air pollution, airborne dirt and dust or pollen in the air, discoloring and swelling of the sensitive skin around your eyes can happen.One good way to make sure that dark bags under eyes go away is to make sure that you get a full night of restful sleep each day.Wearing a high protection factor sunscreen and sunglasses when outdoors may also help to safeguard the skin.

To have a healthy beautiful skin you must make sure that your diet include plenty of whole grain products, fresh vegetables christian louboutin paris soldes and fish.A lot of water should be drunk daily to ensure that the skin is properly hydrated but drinks like alcohol, coffee and caffeinated colas ought to be avoided.Taking a supplement that contains vitamins, minerals and other essential nutrients will help to remove dark bags under eyes.Placing a cold compress over the eye area will have the immediate but shortterm effect of reducing the eye bags.A traditional cure is to apply chilled slices of cucumber on the eyes.

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Heeled shoes operating race


July eight, red square in central moscow held an uncommon sports occasion:Louboutin shoes sprint.Minimum of 70 9 cm young girl wearing high michael kors taschen günstig heels, scrambling for the provisions of 80 meters from the finish, the best score of 13.52 seconds.

Provisions of your competitors, each contestant shall not wear leather shoes, heel height less than 9 cm, 10 cm high heels and a few players, iaelorssuheonlineshop some 12 cm higher, and a few even up to 16 cm.Distance of 80 meters from red square, the starting point to the opposite finish in the moscow state university, bonus of 10 million rubles, a total of 70 girls in moscow to take part in quite a few groups in turn.

1 contestant mentioned: "I wear christian louboutin higher heels for practically ten years old, is now wearing high heels shoes just isn't as comfy.In urban circumstances, can not run will not be adequate, and in some cases to recover the public automobiles, from time to time late for university courses. "

Earlier, russia also has held related christian louboutin pumps highheeled all over the sprint race.July 2 this year, held in yekaterinburg 80 meters high heels sprint race, the most beneficial performance was 13.9 seconds.The close to future, st.Petersburg and novosibirsk city may also hold equivalent events.

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Alpha induces tumor necrosis and tumor cheap beats by dre solo regression without systemic toxicity


Tumortargeted gene delivery of tumor necrosis factor induces tumor necrosis and tumor regression without systemic toxicity

Ralf kircheis1, elinborg ostermann1, markus f wolschek2, 3, cornelia lichtenberger3, christine maginlachmann1, lionel wightman1, malgorzata kursa1 and ernst wagner1received 14 may 2002.

Top of pageabstractwe have recently developed surfaceshielded transferrin(Tf delivery systems that target reporter gene expression to distant tumors after systemic application.In the present study, we used surfaceshielded tf complexes for delivering the gene for a highly potent cytokine, tumor necrosis factor(Tnf tnf is known for its ability to induce hemorrhagic tumor necrosis and tumor regression.However, the therapeutic application of tnf is hampered by its high systemic toxicity dictating the need to target tnf activity to the tumor.Systemic application of surfaceshielded tf complexes with the tnf gene resulted in preferential expression of tnf in the tumor without detectable tnf serum levels, in contrast to the application of nontargeted complexes.Tumortargeted tnf gene delivery induced pronounced hemorrhagic tumor necrosis and inhibition of tumor growth in three murine tumor models of different tissue origins, neuro2a neuroblastoma, metha fibrosarcoma, and m3 melanoma, with complete tumor regressions observed in the metha model.No systemic tnfrelated toxicity was observed due to the localization of the tnf activity to the tumor.Targeted gene therapy may be an attractive strategy applicable to highly active, yet toxic, molecules such as tnf

Keywords:Tumor necrosis factor;Tnf gene therapy;Tumor targeting;Polyethylenimine;Pei;Nonviral gene transfer;Transferrin

The potential of gene therapy to target the expression of therapeutic genes to the desired target cells makes it particularly attractive for cancer treatment.However, although there are vectors available with high transfection efficacy in cell culture, 1, 2, 3, 4 efficient and targetspecific gene delivery in vivo is still the major challenge in gene therapy.In vivo gene delivery has to overcome additional obstacles such as anatomical size constraints and nonspecific interactions with biological fluids and nontarget cells.Recently, we have developed surfaceshielded transferrin(Tf gene delivery systems that are able to target reporter gene expression to distant tumors after systemic application in murine models.5, 6, 7 In the present study, we have employed these gene delivery systems to target gene expression of a highly potent, yet highly toxic, molecule tumor necrosis factor(Tnf to distant tumors with the aim to separate the antitumor activity of tnf from its systemic toxicity.

In the present study, surfaceshielded tf gene delivery systems were used for the first time to target gene expression of a highly potent effector molecule to distant tumors following systemic application.Tnf expression localized to the tumor resulted in hemorrhagic tumor necrosis and inhibition of tumor growth without systemic tnfrelated toxicity.

Top of pagematerials and methodschemicals and expression vectorspei, branched, 25 kda, and linear pei, 22 kda, were obtained from aldrich(Milwaukee, wi)And mbi fermentas(St.Leonrot, germany), respectively.Tf(25kDa)Synthesis has been described previously.7

The plasmids pcmvl, pcmv and pgsmutnf coding for luciferase,(And murine tnf respectively, and the nonexpressing plasmid psp65 were purified by elim biopharmaceuticals(San francisco, ca).Endotoxin levels were u/50 dna as determined by limulus amebocyte lysate assay(Biowhittaker, walkersville, md).

Cells and animalsmurine neuro2a neuroblastoma cells were cultured in rpmi 1640 medium/10% fcs.Murine m3 melanoma cells were cultured in ham's f10 chpdrdrebts medium/15% horse serum/5% fcs.Murine metha fibrosarcoma cells(Kindly provided by dr fichtner, maxdelbr center, berlinbuch, germany)Were passaged in vivo as intraperitoneal ascites in syngeneic balb/c mice.A/j, dba/2, and balb/c mice(7 weeks, female)Were purchased from harlan(Bicester, uk).

Preparation of transfection complexespei/dna complexes were prepared as described.7 Plasmid DNA(200 was flashmixed with PEI(22 kDa)Atn/p(N/p ratio of pei nitrogen to dna phosphate)In 20 mm hepes(Ph for isoosmolarity, glucose was added to a final concentration of 5%(Wt/vol).The potential of transfection complexes was measured using a malvern zetasizer 3000 as described previously.6

Tf complexes were prepared as described previously7 by flash mixing of dna(200 withthe polycation solution(Containing three parts pei and one part tf at n/p complexes were prepared in 20 mm hepes(Ph glucose or 0.5 HBS(75 mM NaCl, 20 mM HEPES, pH glucose.

Determination of luciferase reporter gene expression after systemic applicationa/j mice were injected subcutaneously with 1 neuro2a cells.After 2 weeks, tumors had reached 10 in size;Transfection complexes(250 with the pCMVL plasmid were injected into the tail vein.Animals were sacrificed by cervical dislocation at indicated time points.Organs were resected, homogenized in250 mm tris buffer, ph 7.5, using an IKA Homogenizer, frozen in liquid nitrogen, and stored at Luciferase activity in the tissue lysate was measured using a Lumat LB9507 instrument(Berthold, bad wildbad, germany)As described previously.5 Luciferase background(100 RLU)Was substracted from each value and transfection efficacy is expressed as rlu(Relative light units)Per organ.One million rlu correspond to 2 ng of luciferase.

Therapeutic modelsa/j mice were injected subcutaneously with 1 syngeneic neuro2a tumor cells, dba/2 mice were injected with 1 m3 cells, and balb/c mice were injected with 2 metha cells.Starting at indicated time points after tumor setting(Tumor diameter, 5 mm), transfection complexes containing the plasmid for murine TNF for a control plasmid for or the nonexpressing pSP65 plasmid were injected into the tail vein.Tumor size and body weight of the animals were monitored.Differences in Tumor growth were statistically analyzed using oneway anova(Tumor growth)And contingency tables(Tumor necrosis, complete tumor regression)Analyzed by chisquare test and fisher's exact test, using graphpad prism software.

Histologyfor immunohistologicAl stAining,Cryosections(7 on microslides were fixed inCold Acetone.Endogenous peroxidAse wAs quenched By incuBAting the slides in hydrogen peroxide(SigmA, st.Louis, mo;0.03% in PBS), slides were Blocked with 1% BSA for 15 minutes, with 10% goAt serum for 15 minutes, And suBsequently with DAKO Biotin Blocking System(DAko,CArpinterA,CA).Following incuBAtion with either rAt Anti mAcrophAge f4/80 mAB(CloneCl:A31, 10 serotec, oxford, uk)Or rAt AntiCd11B mAB(Clone m1/70, 6.25 PhArmingen, SAn Diego,CA), detection wAs performed with diAminoBenzidine(DAB)Using A Anti igg hrp detection kit(PhArmingen)According to the mAnufActurer's instructions.HemAtoxylin(Merck, ViennA, AustriA)StAining wAs done using A stAndArd procedure.Slides were mounted with entellAn new medium(Merck)And evAluAted under An olympus(ViennA, AustriA)Bh2 microscope equipped with An olympus dp12CAmerA.An unspecific expression profile with high luciferAse expression in the lungs And other mAjor orgAns wAs oBserved with pei22/dnAComplexes(Fig 1A).InContrAst, surfAceshielded tfComplexes resulted in specific expression in the tumor, whereAs expression in the lungs And other orgAns wAs drAmAticAlly reduced(Fig 1B).The kinetics of luciferAse reporter gene expression wAs meAsured following single orRepeAted ApplicAtionof surfAceshielded tfComplexes in the tAil vein of tumorBeAring mice.Gene expression wAs trAnsient, with the single ApplicAtion showing A peAk At 48 hours, thereAfter decreAsing to very low expression levels At dAy 4, And noLuciferase activitywAs detectABle At dAy 7.RepeAted ApplicAtion(dAys0 And 1)Resulted in higher expression levels At All time points, And significAntLuciferase activitywAs still detectABle in the tumor 1 week After ApplicAtion(Fig 1c).NontArgeted pei22/dnA(A)Or targeted tfComplexes(B)Containing the pcmvl plasmid were injected into the tail vein of neuro2a tumorbearing mice.Luciferase activity(Rlu/organ;Mean was measured 24 hours after injection.C: Kinetics of gene expression After systemic ApplicAtion of tArgeted TfComplexes. TrAnsfectionComplexesContAining 50 of DNA were injected once (dAy0)Or twice(dAys0 And 1).Luciferase reporter gene expression was measured at indicated time points.Animal number was n per time point.

Full figure and legend(328K)

Localization of tnf beats by dre sale online activity to the tumorthe aim of this study was to target gene expression of the highly potent cytokine tnf to the tumor in order to reduce the systemic toxicity observed with systemic application of tnf using nontargeted pei22/dna[tnf complexes resulted in an unspecific gene expression pattern with highest tnf expression detected in the lungs, followed by the liver, heart, and tumor.Moreover, application of pei22/dna[tnf complexes was associated with significant tnf serum levels(Fig 2a).In control animals receiving similar pei22/dna complexes with the reporter gene instead of the tnf gene, no significant tnf levels were found(Data not shown).Lower tnf levels were found in liver and spleen.Importantly, no significant tnf levels were observed in the serum(Fig 2b).In comparison, tnf expression after application of nonshielded pei22/dna[tnf complexes was even shorter with peak levels in the lungs and serum 24hours after application, followed by a rapid decrease(Fig 2a, insert).A/j mice bearing a subcutaneously growing neuro2a tumor were injected with nontargeted(A, c, e)Or targeted(B, d, f)Transfection complexes containing a plasmid for mutnf or the nonexpressing plasmid psp65(C expression of murine tnf(Pg/organ)In the major organs and tumor, and tnf serum levels(Pg/ml)24 hours after single application were measured by ELISA(A, b, inserts:48 hours after single application).Body weight(C, d)And tumor size(E, f)Of animals(Eight per group)Treated with repeated application(Arrow heads)Of transfection complexes were monitored.Untreated animals are shown for control.Complexes were applied repeatedly at indicated time points into the tail vein.Control animals were left untreated or injected with complexes having the empty plasmid vector psp65.Body weightof the animals, as an indicator of TNF toxicity, was monitored during treatment (Fig 2, c and d). A significant drop inBody weightof 10% was found with nonshielded PEI/DNA[TNF complexes after the first two applications (P tnf versus control) (Fig 2c).There was no further weight loss after the following applications;However, in one out of two experiments, nonshielded pei/dna [tnf complexes resulted in the death of 2/8 animals.Nontargeted pei/dna[tnf complexes also inhibited tumor growth(P tnf versus untreated control) (Fig 2e), however, to a lesser extent compared to the targeted complexes(P targeted tnf versus nontargeted tnf complexes with the psp65 control plasmid did not significantly inhibit tumor growth;Similar results were observed with additional control complexes with the reporter gene(Data not shown).

The most striking effect of the tnf gene therApy wAs theInduction of hemorrhagic tumor necrosis(Fig 3, A And B).More thAn 80% of the tnf AnimAlsDeveloped hemorrhAgic tumor necroses, whereAs hemorrhAgic tumor necrosis wAs virtuAlly not found inControl AnimAls(P tnf versusControl)And only rArely found in psp65 or AnimAls(P tnf versus or psp65) (Fig 3c).Necrosis wAs most pronounced in theCenter of the tumor.However, there wAs only in very fewCAses AComplete erAdicAtion of the tumor in this model.AfterCessAtion of treAtment, tumorCells locAted outside of the necrotic AreAs stArted to regrow.The therApeutic efficAcy of tArgeted tnf geneDeliveryCorrelAted with the numBer of ApplicAtions.Preexperiments on A smAll numBer of AnimAls with single ApplicAtion showed only moderAte therApeutic effect And high vAriABility Among the AnimAls not Allowing significAntConclusions(DAtA not shown).ToCompAre the therApeutic efficAcy ofDifferent treAtment regimes, the t/c rAtio(Tumor growth in treAted groupCompAred toControl group)WAs meAsured AfterDifferent numBers of ApplicAtions.A rAtio of t/c is generAllyConsidered indicAtive for A significAnt therApeutic effect.Four ApplicAtions of tnf gene therApy showed A significAnt therApeutic effect with A rAtio of t/c however, there wAs A vAriABility in the treAtment group.Six or eight ApplicAtions of tArgeted tnf gene trAnsferComplexes resulted in A t/c rAtio of 25% or 13%, respectively, indicAting higher efficAcy with higher numBer of ApplicAtions.Furthermore, increAsing the numBer of ApplicAtion Also minimized the vAriABility Among the AnimAls in the treAtment groups(Fig 3d).Further experimentsDemonstrAted thAt hemorrhAgic tumor necrosis And inhiBition of tumor growth were induced only By trAnsfectionComplexesCoding for tnf whereAsCytokines with A more indirect immunemediAted mechAnism of Action, such As interleukin2 or interferonDid not show significAnt efficAcy in this Aggressive tumor model(DAtA not shown).Neuro2A tumorBeAring A/j mice were injected with tArgeted trAnsfectionComplexesContAining expression plAsmids for(A)Mutnf or(B)C:Summary table.Induction of hemorrhagic tumor necrosis.Results of three independent experiments are shown. TNF versus pSP65, AndControl.D: TherApeutic efficAcy of TNF gene therApy AfterDifferent treAtment regimes (Four, six, or eight applications)Was estimated by the t/c ratio(tumor growth group versusControl group) AtDAy 21.

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Colorado pot accidents spur call for childproof packaging


Robin hackett, owner of botana care in northglenn, colo., putting medical marijuana suckers into a sample zipperlock baggie. (Joe amon/the denver post)

From early 2005 to late 2009, children's hospital colorado had exactly zero emergencyroom visits by kids who had ingested marijuana.In the following two years, when medical marijuana became legal in colorado and federal officials backed off prosecution, it had 14.

Pioneering studies of emergency room charts by colorado doctors show looser pot laws leading to childhood poisonings, often ristianlbtinaussuressoldes from mistakenly eating tantalizing"Edibles' like gummy worms or brownies.

Those doctors are now helping lead the charge for mandatory safety packaging as colorado gears up for even broader legal sales of pot with recreationalmarijuana stores.

"We've seen a dramatic increase in pediatric exposure, ' said dr.George wang, a children's er doctor who also works with denver health's rocky mountain poison and drug center.

Calls about potential marijuana exposure at all ages have doubled since 2009 at the poison center.

Safety packaging, as in other medicines,"Is a supplement to careful parenting that has been shown to work,' said wang's colleague, dr.Michael kosnett. "There are solutions available right now.'

And the marijuana christian louboutin platforms sale industry agrees, up to a point.

Many industry members favor sending goods out the store door under tamperproof seal.But they would rather not break each individual joint or candy into a hightech, lockable bag whose cost up to $7, even in bulk might approach the price of the brownie inside.

Have to buy so much tamperproof packaging that people will just make it themselves at home,' said robin hackett, coowner of botana care, a medicalmarijuana store in northglenn. "The challenge is with a pound of butter and some cannabis, anybody can make edibles.'

Hackett and other members of the medical marijuana industry group say lockboxes and larger locking bags that buyers can use to transport larger purchases home should solve most of the safety problems.

Breaking everything down into smaller tamperproof bags is a landfill problem and unnecessary expense, hackett said.Botana care counsels patients with children about safety, and keeps track of who has kids as a reminder.

"Unfortunately, we can't christian louboutin homme write laws around dumb, ''"She said.

None of the accidentally poisoned children has died, wang and kosnett said.

There are serious medical consequences for small children, though, even while marijuana advocates say an adult"Overdose' of pot is nearly impossible.

Prescribed dosages of thc, the active ingredient in marijuana products used to control nausea from chemotherapy, is between 4 and 12 milligrams for children ages 2 to 4, based on body surface area.Some"Edibles' have 300 milligrams of thc, kosnett said.

The researchers say individual safety packs would be best, but the current recommendation of all items leaving the store in one secure package is"Better than nothing. "

Because there is no clear reporting category for marijuana poisonings, doctors have to cull through files to count cases.Presbyterian/st.Luke's, which operates rocky mountain hospital for children, said it does not track similar cases.

The cases studied at children's included decreased levels of consciousness and breathing trouble.Children can also vomit from ingesting too much of a strong substance and aspirate the vomit.

The median age in the 14 patients was 3 years, and the range was 8 months to 12 years, according to an abstract of the research published in the journal clinical toxicology.

Childingested pot is also dangerous because er doctors aren't looking for it as a cause of any symptoms they see, wang said.That can lead to invasive and expensive diagnostic efforts, such as a spinal tap or ct scan, if parents are embarrassed or scared to mention the true cause.

"When children get admitted to the icu, that's serious, ' kosnett said.Symptoms may appear similar to meningitis, for example.

Safety packaging and parental prevention should be noncontroversial, said dr.Robert brockmann, president of the colorado academy of family physicians, especially as newly legal recreational use will greatly expand the supply.

"None of that information is being disseminated when it's dispensed,' brockmann said. "It's like liquor or prescription medications, or anything else you don't want your kids to get into.'

Such measures have cut pediatric poisonings in various categories by 40 to 90 percent over the decades, he said.

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Clorox reports solid sales and margin growth in first quarter


Net cash provided by operations increased to $208 million from $131 million in the yearago quarter.The yearoveryear increase was due primarily to favorable changes in working capital.For the full fiscal year, clorox anticipates free cash flow of about 9 percent to 10 percent of sales.The company defines free cash flow as cash provided by operations less capital expenditures.In september, the company issued $600 million of 10year senior notes, temporarily increasing its cash balance prior to the midoctober repayment of other maturing longterm debt of $350 million.The company's cash balance is expected to return to a more normalized level by dec.31.

11% pretax earnings increase

Volume growth in the segment was driven primarily by high doubledigit growth in the professional products business, due to the benefit of acquisitions made in fiscal 2012 and a doubledigit increase in the base business.Shipments in the home care business were about equal to the year ago quarter, with alltime record shipments of clorox disinfecting wipes, offset by declines on pinesol products due to a price increase in the fourth quarter of fiscal 2012.Home care sales increased due to the benefit of beats by dre uk price increases.Laundry volume decreased due to lower shipments of clorox 2 stain fighter and color booster, partially offset by the highest volume growth of bleach in more than two years.Segment sales outpaced volume largely due to the benefit of price increases.Pretax earnings growth was driven primarily by higher sales and the benefit of strong cost savings, partially offset by unfavorable product mix.

19% beats by dre sale online pretax earnings increase

The segment's volume and sales decreases were driven primarily by declines in the charcoal business, reflecting the impact of price increases earlier in the calendar year and the comparison to very strong merchandising and volume growth in the yearago quarter.Cat litter volume declined due to price increases implemented in the fourth quarter of fiscal 2012 and increased competitive activity.Glad volume was up, with continued strong gains in premium trash bag products such as glad odorshield trash bags with febreze.The variance between the changes in segment volume and sales was primarily due to the benefit of price increases.Pretax earnings growth was driven largely by strong cost savings, partially offset by lower sales.

2% pretax earnings increase drdrebtshdphones

Volume was up in the food business driven primarily by higher shipments of hidden valley products, offset by lower shipments of kc masterpiece products due to strong competitive activity.Volume declined in the water filtration business due to lower shipments of faucet mount products, as well as lower brita bottle shipments, compared to strong volume behind the new product launch in the prioryear quarter.Burt's bees shipments declined slightly, following strong growth in the yearago period, behind a robust pipeline of new products.Burt's bees delivered doubledigit retail sales growth.Segment sales grew primarily due to the benefit of price increases.Pretax earnings growth reflected the benefit of cost savings.

30% pretax earnings decrease

Volume declined in the segment largely due to the exit of nonstrategic export businesses.Base business in latin america grew both volume and sales, while canada volume and sales declined slightly.Segment sales increased primarily due to price increases, partially offset by unfavorable foreign exchange.The pretax earnings decline of $12 million was due to the inflationary impact on manufacturing and logistics costs, unfavorable foreign exchange and expenses associated with it systems implementation in latin america.Clorox markets some of the most trusted and recognized brand names, including its namesake bleach and cleaning products, clorox healthcare", HealthLink, Aplicare and Dispatch products, Green Works naturally derived home care products, PineSol cleaners, Poett home care products, Fresh Step cat litter, Glad bags, wraps and containers, Kingsford charcoal, Hidden Valley and KC Masterpiece dressings and sauces, Brita waterfiltration products, and Burt's Bees and gud natural personal care products.Nearly 90 percent of the company's brands hold the no.1 or No.2 market share positions in their categories.The company's products are manufactured in more than two dozen countries and marketed in more than 100 countries.Clorox is committed to making a positive difference in the communities where its employees work and live.Founded in 1980, the clorox company foundation has awarded cash grants totaling more than $87 million to nonprofit organizations, schools and colleges.In fiscal year 2012 alone, the foundation awarded $3.5 million in cash grants, and Clorox made product donations valued at $15 million.

Forwardlooking statementsthis press release contains"Forwardlooking statements"Within the meaning of section 27a of The securities act of 1933, as amended(The securities act),AndSection 21E of the Securities Exchange Act of 1934, as amended(The exchange act),Andsuch forwardlooking statements involve risksAnduncertainties.Except for historical information, matters discussed above, including statements about future volume, sales, costs, cost savings, earnings, cash flows, Plans,objectives, expectations, growth, or profitability, are forwardlooking statements based on management's Estimates,assumptionsAndprojections.Words such as"Will," "Could," "May," "Expects," "Anticipates," "Targets," "Goals," "Projects," "Intends," "Plans," "Believes," "Seeks," "Estimates,"And variations on such words,Andsimilar expressions, are intended to identify such forwardlooking statements.These forwardlooking statements are only predictions, subject to risksAnduncertainties,Andactual results could differ materially from those discussed above.Important factors that could affect performanceAndcause results to differ materially from management's expectations are described in the sections entitled"Risk factors"And"Management's DiscussionAndAnalysis of Financial ConditionAndResults of Operations"In the annual report on form 10k for the fiscal year ended june 30, 2012, as updated from time to time in the company's sec filings.These factors include, but are not limited to: the company's costs, including volatilityAndincreases in commodity costs such as resin, diesel, chloralkali, sodium hypochlorite, highstrength bleach, agricultural commoditiesAndother raw materials;Increases in energy costs; the ability of the company to implementAndgenerate expected savings from its programs to reduce costs, including its supply chain restructuringAndother restructuring plans;Supply disruptions or any future supply constraints that may affect key commodities or product inputs;Risks inherent in relationships with suppliers, including solesource or singlesource suppliers;Risks related to the handling and/or transportation of hazardous substances, including, but not limited to, chlorine;The success of the company's strategies; the ability to manageAndrealize the benefits of joint venturesAndother cooperative relationships, including the company's joint venture regarding the company's Glad plastic bags, wrapsAndcontainers business,Andthe agreements relating to the provision of information technology, procure to payAndother key services by third parties; risks relating to acquisitions, mergersAnddivestitures,Andthe costs associated therewith; risks inherent in maintaining an effective system of internal controls, including the potential impact of acquisitions or the use of thirdparty service providers,Andthe need to refine controls to adjust for accounting, financial reportingAndother organizational changes or business conditions; the ability of the company to successfully manage tax, regulatory, product liability, intellectual property, environmentalAndother legal matters, including the risk resulting from jointAndseveral liability for environmental contingenciesAndrisks inherent in litigation, including class action litigationAndInternational litigation; risks related to maintainingAndupdating the company's information systems, including potential disruptions, costsAndthe ability of the company to implement adequate information systems in order to support the current businessAndto support the company's potential growth;The ability of the company to develop commercially successful products that delight the consumer; consumerAndcustomer reaction to price changes;Actions by competitors;Risks related to customer concentration; customerspecific ordering patternsAndtrends;Risks arising out of natural disasters;The impact of disease outbreaks, epidemics or pandemics on the company's, suppliers' or customers' operations;Changes in the company's tax rate; unfavorable worldwide, regional or local general economicAndmarketplace conditionsAndevents, including consumer confidenceAndconsumer spending levels, the rate of economic growth, the rate of inflation or deflation,Andthe financial condition of the company's customers, suppliersAndservice providers; foreign currency exchange rate fluctuationsAndother risks of international operations, including governmentimposed price controls; unfavorable political conditions in the countries where the company does businessAndother operational risks in such countries; the impact of the volatility of the debtAndequity markets on the company's cost of borrowing, cost of capitalAndaccess to funds, including commercial paperAndits credit facility; risks relating to changes in the company's capital structure, including risks related to the company's ability to implement share repurchase plansAndthe impact thereof on the company's capital structureAndearnings per share; the impact of any unanticipated restructuring or assetimpairment chargesAndthe ability of the company to successfully implement restructuring plans;Risks arising from declines in cash flow, whether resulting from declining sales, higher cost levels, tax payments, debt payments, share repurchases, higher capital spending, interest cost increases greater than management's expectations, interest rate fluctuations, increases in debt or changes in credit ratings, or otherwise; the costsAndavailability of shippingAndtransport services;Potential costs in the event of stockholder activism;Andthe company's ability to maintain its business reputationAndthe reputation of its brands.

The company has disclosed information related to sales growth, gross margin and diluted eps on a nongaap basis to supplement its condensed consolidated statements of earnings presented in accordance with gaap.These nongaap financial measures exclude certain items that are included in the company's results reported in accordance with gaap, including charges associated with simplification of the company's supply chain and other restructuringrelated charges and the impact of foreign exchange and foreign currency exchange transactions and acquisitions.

Management believes that these nongaap financial measures provide useful additional information to investors about current trends in the company's operations and are useful for periodoverperiod comparisons.These nongaap financial measures should not be considered in isolation or as a substitute for the comparable gaap measures.In addition, these nongaap measures may not be the same as similar measures provided by other companies due to potential differences in methods of calculation and items being excluded.They should only be read in connection with the company's condensed consolidated statements of earnings presented in accordance with gaap.

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Cleaning out your clothes closets


Keeping your wardrobe up to date is easy if you start by sorting through all your unwanted clothing and donate it to charities.

I always looking for people to give my daughter outgrown clothes to.Every 2 months or so my daughter and i sort through her clothes and make one pile for charities, and another pile for the consignment store.

I found to keep your child wardrobe up to date(And your own for that matter), you have to regularly go through and weed out the outgrown and unwanted clothes.If you don you just end up with closets and bags, and often even piles of unwanted clothing that will never be worn again.After awhile it just gets to the point where you don know where to start.Making cleaning out your clothes closet a regular routine will save you a lot of time and energy later down the road and even help you to keep your wardrobe up to date.It takes us an hour at the most to go though our clothes and sort them out.When you do this regularly you find you don need as many pieces of clothing you maybe thought you did, and you actually wear and enjoy the clothing you do have.My winter and summer clothing both fit into half of our small closet, and i no longer find my family outgrown and unwanted clothing stored all around the house.

So what can you do with unwanted clothes?The easiest thing to do is donate them to charities.Good will, salvation army, women shelters, and other charities are always looking ristianlbtinhomme for donations, and they will often come right to your front door to pick up your unwanted clothing for you.

If your clothing is still in really good condition and you want to take the time, you christian louboutin chaussures pas cher can consign your inseason clothing and make a few extra dollars.Check your local yellow pages for consignment store listings.

I saw a great idea on tv that i thought would be fun to try sometime.A group of women got together in someone home and brought their unwanted clothing to share with each other.Someone took the time to organize the clothing and display it attractively, christian louboutin peep toe pumps and then the women got together and took home their pick of clothing.They could take whatever they liked as long as they had contributed some of their own clothing.It was neat how the event was organized.The women had makeshift dressing rooms where they could try on clothing and refreshments were served while the women visited with one another.It looked like a lot of fun and a great way to update your wardrobe.This can also be done with children clothing and toys.

The next time you look into your family closets and see even one article of clothing that hasn been worn in the past 6 months, maybe it time to sort through them and pass some of the clothing along.The more organized your closets are, the more easily you and your family will be able to find the clothing you truly love to wear.

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