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In this laboratory you will use some basic tools of molecular biology to gain an understanding of some of the principles and techniques of genetic engineering. In the first part of the lab, you will use antibiotic-resistance plasmids to transform Escherichia coli. In the second part, you will use gel electrophoresis to separate fragments of DNA for further analysis.

Paul Andersen explains the two major portions of the molecular biology lab in AP Biology. He starts by discussing the process of transformation. He explains how you can use the pGLO plasmid to produce glowing E. coli bacteria. He then describes how you can use restriction enzymes and the process of gel electrophoresis to cut and separate DNA.

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