What are the advantages of microinjection? - 0 views
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sairoy on 30 Aug 19Microinjection is a method that requires a direct injection of DNA into the nucleus of a cell with the aid of a very fine needle. This recombinant DNA absence the genes required for retroviral replication and assembly of viral particles. The target is commonly a living cell but may also include intercellular space. Microinjection being an easy and simple mechanical process, usually including an inverted microscope with an amplification power of around 200x (though sometimes it is performed using a cut-up stereo microscope at 40-50x or a traditional compound upstanding microscope at similar power to an inverted model). Advantages of microinjection: 1. The frequency of a stable combination of DNA is far better as compared to other methods. 2. The DNA injected in the process is subjected to less extensive modifications. 3. The method is effecting in transforming primary cells as well as cells in established cultures. 4. Mere precise integration of the recombinant gene is a limited copy number can be obtained.