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Microinjection is a method that requires a direct injection of DNA into the nucleus of a cell with the aid of a very fine needle. This recombinant DNA absence the genes required for retroviral replication and assembly of viral particles. The target is commonly a living cell but may also include intercellular space. Microinjection being an easy and simple mechanical process, usually including an inverted microscope with an amplification power of around 200x (though sometimes it is performed using a cut-up stereo microscope at 40-50x or a traditional compound upstanding microscope at similar power to an inverted model). Advantages of microinjection: 1. The frequency of a stable combination of DNA is far better as compared to other methods. 2. The DNA injected in the process is subjected to less extensive modifications. 3. The method is effecting in transforming primary cells as well as cells in established cultures. 4. Mere precise integration of the recombinant gene is a limited copy number can be obtained.

"The new WPI MICRO-ePORE™ pinpoint cell penetrator is a simple and versatile system that can be used for efficient microinjection of a diverse array of compounds and biomolecules into oocytes and pre-implantation stage mammalian embryos. Patent pending Flutter Electrode Technology assists in small, clean, precise membrane penetration without tearing or damaging the membrane. Benefits Increase viability of injected embryos Flexible system Integrates with WPI's PV820/PV830, as well as other popular microinjection systems, like the Eppendorf Femtojet® and the Narishige injectors (Femtojet® 4I is a registered Trademark of Eppendorf.) Microinjection of diverse compounds and biomolecules - DNA, RNA, proteins Pre- and post-implantation in embryos of various species - mice, rodents, monkeys, bovine, pigs, zebrafish, etc. Easy to operate - Hands-free operation with foot switch control User adjustable frequency and voltage - four programmable protocols Small, portable unit saves precious benchtop space Works with all major inverted microscopes"

A transgenic animal is one whose genome has been altered by the transfer of a gene or genes from another species. In biomedical research, in the laboratory, transgenic animals are routinely used as models. Over 95 percent of those users are genetically modified rodents, predominantly mice. Transgenic animals have many Utilities which made them much superior to the normal animals. Utilities of Transgenic Animals: * During medical research, transgenic animals are mostly used to identify the functions of some specific factors in complex homeostatic systems. These can be through the over- or under-expression of a modified gene (the inserted transgene). * In the toxicology as the responsive test animals for the detection of toxicants. * In mammalian developmental genetics. * In molecular biology, this analysis of the regulation of the gene expression, make use of the informative evaluation of some specific genetic change, at the level of the whole animal. * While in the pharmaceutical industry, for the targeted production of pharmaceutical proteins, and during the drug production also on the product efficacy testing. * In biotechnology, as producers of specific proteins. * For the genetically engineered hormones to increase milk yield, meat production. * In the cloning procedures to reproduce some specific bloodlines in the genetic engineering of the livestock, and in the aquaculture that affects the modification of animal physiology and/or anatomy, * Developing animals specially created for use in xenografting.

Gene transfer technique is used very frequently both in basic research and applied biology. The delivery of DNA into animal cells is a fundamental and fixed procedure. It has become anecessary tool for gene cloning, the study of gene function and regulation and the production of small amounts of recombinant proteins for analysis and verification. Gene transfer experiment helps to launch the genetic construct (or transgene) in the recipient cells or to disrupt or inactivate particular endogenous genes resulting in a loss of function. There are many utilizations of gene-transfer like large-scale commercial production of recombinant antibodies and vaccines and gene medicine or gene therapy. They range from the use of mammalian and insect cell cultures to transfer of DNA into human patients for the correction or prevention of disease.

The concept of gene transfer between cells first existed in bacteria; they are capable of at least four natural forms of genetic exchange. The genetic transfer technique is used very widely in basic research and applied biology. The delivery of DNA into an animal cell is a very established and fundamental procedure. It has become a very crucial tool for gene cloning, the study of gene function and the regulation and production of small amounts of recombinant proteins for analysis and verification. It helps to express the introduced genetic construct in the recipient cells or inactivate particular endogenous genes.

Electroporation is a microbiology technique where an electrical field is applied to cells to increase the permeability of the cell membrane. It allows chemicals or DNA to be instigated into the cell.

Cell manipulation is mainly done with the purpose of shaping and manipulating the gene in the cells of the organism and even to change the functioning of the cells.

The concept of gene transfer between cells first existed in bacteria; they are capable of at least four natural forms of genetic exchange. The genetic transfer technique is used very widely in basic research and applied biology. The delivery of DNA into an animal cell is a very established and fundamental procedure. It has become a very crucial tool for gene cloning, the study of gene function and the regulation and production of small amounts of recombinant proteins for analysis and verification. It helps to express the introduced genetic construct in the recipient cells or inactivate particular endogenous genes.

Microinjection for transgenic animals is a direct method to introduce DNA into either cytoplasm or nucleus. DNA microinjection methods are used to transfer the genes between animals. This is quite a popular technique for creating transgenic organisms. DNA microinjection is also known as the pronuclear microinjection. In this process, the genes must be transmitted through the germline, including germ cells of the animal that contains the same modified genetic material. Transgenic animals are those consequences of experimental application, wherein the DNA integrated into their germline. The first transgenic animals were produced in the year of 1980. Mice were one of the first transgenic animals at that time. https://www.wpiinc.com/micro-epore-pinpoint-cell-penetrator

Cell manipulation using optical traps was first indicated by Bell Laboratories using visible argon laser light and infrared light. Movement of a micron-sized dielectric particle is convinced by the direct transfer of photon momentum to a non-absorbing particle during refraction and reflection, which is the radiation pressure. The optical force from a high intensity focused Gaussian profile laser has scattering (the process in which electromagnetic radiation or particles are deflected diffused.) and gradient force components, which gives a valid point of stable equilibrium near the beam focus. Particles are trapped being across by the gradient force, while around an axis motion of the particle is controlled by the scattering forces. The wavelength of light (dp>> λ) is less than for cells, ray optics have been used to derive the radiation force from scattering of the incident light momentum.

Fiber Optic cable has a complex design. This type of cable has an outer optical coverage that surrounds the light and traps it within a central core. This type of cable includes standard optical fibers with 2 connectors and furcation tubing, bifurcated fiber, cross fiber connectorized assemblies, long length assemblies to name a few. As a company, WPI provides the product developers and researchers with the UV basic attenuation data by fiber length. This is post manufacturing for starters. One such proprietary formulation of WPI fiber resists UV degradation and allows for deep UV down to 185 nm. However, the UV performance of the fiber is dependent on two parameters like the basic attenuation and solarization induced loss over the usage time. When you purchase the UV qualified fiber from WPI, you will receive a certificate. This certificate states for the UV transmission of fiber optic assemblies after it has been built. Also at the same time, you will receive a certificate of the induced loss that is generated by continuous exposure to a 30 Watt deuterium bulb of each of the fiber material batch from 185-360 nm. As we consider the low volume for the flow cell, be it for FIA or HPLC and for the process analysis, WPI has performance advantages. The advantages that WPI provides are as follows: * High UV transmission. * Low internal volume, 2.4 - 24 µL. * 0-10 mL/min flow rate. * Fits 500 and 600 µm fibers. * Wetted materials of PEEK, fused silica or PTFE. * Liquid core waveguide technology-UV/VIS flow cell.

Microinjection with its term shows very fine needle 'micro' with the function of injecting i.e., 'injection'. This process is mainly used for transferring foreign DNA into a living cell like in a cell or embryo of an animal.

Electroporation is also called as electropermeabilization as a way to introduce some new substance into the cell to change the function of a cell or a piece of coding DNA. The appliance that is used for doing this process is known as electroporator.

Nanoinjection is the process of delivering DNA to a cell through an electrical medium that is being conducted by a medical device termed as a nanoliter injector » or nano injector. The main application of the nano injector is in oocyte injections, neo-cortex injections.

MICRO-ePORE is an advanced tool that ensures a much better success rate in the Genetic Transfer process. It works as DNA Microinjection and DNA is inserted into animal cells using microinjection.

Watch as this pair sets up an InVivo microscope chamber. Ideal for live cell imaging, the chambers, along with heaters, carbon dioxide and oxygen controllers and stagetop environments are sold by World Precision Instruments. For more information, contact us at 866.606.1974 (US Toll Free)

TEER measurement stands for TransEpithelial/TransEndothelial Electrical Resistance. TEER measurement denotes measurements related to the cellular monolayer in which different kinds of cells are typically cultured. To understand and confirm the integrity of the monolayer transepithelial electrical resistance measurement is being done.

Microinjection is a method where the foreign DNA transfers between the animals. A transgenic animal is one whose genome has been altered by the transfer of the genes from another species or breed....

If you are looking for a source of cost-effective and high-quality research instruments so you can focus on your scientific journey? Let WPI be your trusted partner as you stock your research laboratory with equipment, surgical instruments, and supplies. Cited in 1000s of notable publications over the last 50 years, WPI offers quality and value to biomedical researchers. Whether you study cell and tissue biology, animal physiology, electrophysiology or perform small animal surgery. WPI's surgical instrumentation offering includes over 1,400 individual Instruments and 11 pre-assembled surgical-specific kits, which are designed for select surgical protocols. WPI also offers researchers and veterinarian surgeons the opportunity to build custom surgical kits for their own needs. WPI's quality line of surgical instruments is made of German steel with German craftsmanship, and it is constructed to our exact specifications. To match the quality of our German line, WPI's intermediate line of surgical instruments also goes through proper passivation with electrolytic polishing. The quality material and craftsmanship used in this line gives you the performance you demand at an economical price. WPI strives to provide you with quality instruments at cost-effective prices so you get more for less. WPI offers surgical instruments and accessories for various applications: * Micro or spring scissors for the dissection of soft tissues, blood vessels, nerve, and ophthalmic procedures * Thumb forceps for compressing or grasping tissues in surgical procedures and handling sterile dressings * Hemostatic forceps to control bleeding in many surgical procedures In addition, WPI also offers surgical adhesives for wound closures, sterilizers and cleaners, and various accessories needed for surgery including biopsy punches, ear punches, loupes, micro drills and more. Surgical instruments need to be sterilized before use to prevent infection. WPI offers a vast range of steril

Optical fiber, thin flexible fiber with a glass core via which light signals can be sent with minute loss of strength. This Optical fiber is also transparent. Optical fibers are mostly used in transmit light between the two ends of the fiber and find wide usage in fiber-optic communication. Here they permit transmission over longer distances and at higher data rates than electrical cables. Fibers are used instead of metal wires because signals travel along with them with less in addition. Optical fibers are also used for illumination and imaging. These fibers typically include a core surrounded by a transparent cladding material with a lower index of refraction Uses of Optical Fibers in various fields: Communication:- i. Used as a medium for telecommunication and computer networking because it is flexible and can be bundled as cables. ii. Advantageous for long-distance communications iii. This fiber can carry many independent channels, each using a different wavelength of light. Sensors:- i. Optical fiber is used to connect a non-fiber optic sensor to a measurement system. ii. These fibers are used as a sensor to measure strain, temperature, and pressure. Power transmission: i. It can be used to transmit power using a photovoltaic cell. ii. it is especially useful for MRI machines, which produce strong magnetic fields. Miscellaneous uses: i. used in imaging optics. ii. Light guides as medical and other applications where bright light needs.

Trying to figure out how to set up your new REMS system? check out on this video. The REMS AutoSampler automates measurements of electrical resistance of transepithelial, transendothelial or Caco-2 cell membranes being grown to confluence on microporous filters of high throughput screening (HTS) 24- and 96-well microplates.