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Long Noncoding RNA as Modular Scaffold of Histone Modification Complex Long intergenic non protein coding rna is a class of long non coding rna in plants molecule with a length of more than 200nt, which is a by-product of RNA polymerase II transcription. It can regulate gene expression at various levels (epigenetic regulation, transcriptional regulation and post-transcriptional regulation, etc.). It is a research hotspot of genetics.

Circular RNAs (circRNAs) are a type of non-coding RNA molecules that lack a 5' cap and a 3' poly(A) tail and form a circular structure with covalent bonds. Distinct from traditional linear RNAs, circRNAs possess a closed circular structure and are abundantly present in the eukaryotic transcriptome. The majority of circRNAs are composed of exon sequences and exhibit conservation across different species, as well as tissue-specific and expression patterns of developmental stage-specific.

Luciferase is a general term for enzymes that can produce bioluminescence in nature, with the most representative ones are F-Luciferase from fireflies and R-Luciferase from renilla. Under the catalysis of luciferase, luciferin can efficiently convert to oxidized luciferin and emit strong fluorescence at the same time. Therefore, researchers used luciferase to develop a highly sensitive and convenient gene reporter system. Currently, this system is widely applied in various fields, including transcription factor binding site and promoter activity analysis, RNA degradation, signal transduction, drug screening, and in vivo animal imaging.

Lentivirus vector is a gene therapy vector developed on the base of HIV-1 (human immunodeficiency virus type 1). Lentivirus vector is a powerful tool for introduction of exogenous genes, with the advantages of wide infection spectrum, effective infection and long-term stable expression of exogenous genes. Now the lentivirus packaging kit, lentivirus packaging system has been widely used in various cell lines for gene overexpression, RNA interference, microRNA research, as well as in vivo experiments.