When considering the factors for development of a cell culture assay there are two major parts that need to be considered: the cell growth conditions and the cell treatment conditions. Many times variables may differ for the two parts of the assay so they need to be kept as separate parts of the assay when considering the factors. In the table below is a list of the factors that one would consider when running an experimental design to develop an assay. The range of the factor is based on literature reference, existing protocols, American Type Culture Collection (ATCC) recommendations, etc. The rationale for why these factors are detailed below.
Factors which may be analyzed using PPT for quality control purposes