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A monoclonal CRISPR/Cas9 knockout cell line is a cell line that has been genetically modified using the CRISPR/Cas9 gene editing technology to create a specific knockout of a target gene. This cell line contains a single, identical copy of the modified gene, making it a monoclonal cell line. The knockout of the target gene allows researchers to study the function of that gene in the cell and its role in various cellular processes. These cell lines are valuable tools for studying gene function, drug discovery, and disease modeling.