from that research , we can know not only traditional ways to identify virus, but also a up to date way to identify them. it Identify an Adenovirus Receptor by using affinity capture and mass spectrometry. it is quite complicated but really cool. i can bet it include really important information !! the one thing thought about more about this research is this up to date method is definately cool but includes too handful and laborious jobs. also, the reaseacher on this paper was really lucky. because they didn't demonstrate Adenovirus use glycoprotein receptor. most of case, receptor is consisted of glycoprotein . not all case.also, it means this method can be used when host cell has only glycoprotein receptors ! but , it is worth to being a few way to identify receptor quite specifically and identifying plycoprotein, most receptor included, scientifically.

i want to know about More detailed work of 5'-RACE . from that research, i could know that 5'-race make a complementary connection with second primer required for PCR mRNA so that provides a primer-binding site upstream of the unknown 5' sequence of the target mRNA !

from that research, i could know about what kinds of other virus which can use mosquitoes for their movement.

from that writing i could find the anwer "why rna virus can maintain their big size?". because they have really complex rigid structure

in this article we can know about unique novel tymoviridae virus. they are characterising about this new virus. one of the most unique feature about them is they can replicate even in mosquitoes!in consider that no mosquitoes have been reported as vectores of plant viruse,this research is quite suprising thing! but something i want to recommend is ...first At the result, it said cell lysates did not react with antisera to known only a few arboviruses of the genera. And said this virus is an uncommon arbovirus . I think the author should define more specially about the subject. and second, this research said that CuTLV virus may be transferred to male mosquitoes feed on plant juices and can started to replicate in the insect also. Because marifviruses can potentially replicate both in plants and in insects but, I can't figure out how this viruse can move into human,(animal) different kinds of species , can make a disease even by male ! I think the writer should more explain about that route. the last thing is There is many pictures .but some of them ,like figure 4&figure 5, are too massy to be hard recognized or unneeded thing. So I think they need more arrangement about picture.

this writing is about mimivirus which have the largest virus and large DNA. its origin come from water and it estimated first host is also a marine viability. mimi virus character is suprisingly, can replicate genetic material by themselves! having cellular machinary combined with bacteria, but not absolutly dependent to host system,just need help just step of its metabolism. i think mimivirus middle stage from parastic alive to dependent form has more possibility