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A Multicomponent Animal Virus Isolated from Mosquitoes: Cell Host & Microbe - 68 views

www.cell.com/...S1931-3128(16)30310-9

shared by kwedul on 02 Sep 16 -

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with each genome segment separately packaged into virions. Although multicomponent genomes are relatively common among RNA viruses that infect plants and fungi, this method of genome organization has not previously been seen in animal viruses (
- kwedul on 21 Sep 16
  
  Is this the only thing required for a genome to be considered multicomponent?
  
  <div class="cArrow"> </div><div class="cContentInner">Is this the only thing required for a genome to be considered multicomponent?</div>
  
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RNA extracted from purified GCXV particles confirmed the presence of a segmented genome (
- kwedul on 21 Sep 16
  
  So what exactly is it about this purified GCXV that shows it is segmented?
  
  <div class="cArrow"> </div><div class="cContentInner">So what exactly is it about this purified GCXV that shows it is segmented?</div>
  
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The mosquito pools for both ACH27 and TR7094 contained multiple viruses capable of replicating in mosquito cells, so we were unable to obtain pure cultures for these isolates (
- kwedul on 21 Sep 16
  
  What is it about there being multiple viruses capable of replicating that made them unable to obtain pure cultures for the isolates?
  
  <div class="cArrow"> </div><div class="cContentInner">What is it about there being multiple viruses capable of replicating that made them unable to obtain pure cultures for the isolates?</div>
  
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...4 more annotations...
Nuclease digestion assays (Figure 1Figure 1B), along with 5′ and 3′ rapid amplification of cDNA ends (RACE), confirmed that all five genome segments were single-stranded, positive-sense RNA (ssRNA+).
- kwedul on 21 Sep 16
  
  What about these tests confirms all five genome segments were single-stranded positive-sense RNA?
  
  <div class="cArrow"> </div><div class="cContentInner">What about these tests confirms all five genome segments were single-stranded positive-sense RNA?</div>
  
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all five segments, (2) segments 1–4, and (3) segments 2–5
- kwedul on 21 Sep 16
  
  Why did they use these three combinations?
  
  <div class="cArrow"> </div><div class="cContentInner">Why did they use these three combinations?</div>
  
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the number of plaques decreased more quickly than expected with dilution of the inoculant) (
- kwedul on 21 Sep 16
  
  Why? What is the significance of this?
  
  <div class="cArrow"> </div><div class="cContentInner">Why? What is the significance of this?</div>
  
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segments 1–5 (passage 1) and segments 1–4 (passage 2)
- kwedul on 21 Sep 16
  
  What about a second passage, including 1-4, makes it brighter?
  
  <div class="cArrow"> </div><div class="cContentInner">What about a second passage, including 1-4, makes it brighter?</div>
  
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