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Contents contributed and discussions participated by Casey Finnerty

Casey Finnerty

Why Norovirus Crops Up on Cruises - NYTimes.com - 0 views

  • Over the past five years, an average of about 14 cruise ships a year have had outbreaks of diarrheal illness, and the culprit is almost always norovirus
  • The best way to avoid it is prevention, and the best prevention is hand washing.
  • The problem, he said, is passengers. “If Grandma is sick when she gets on, she’s going on the cruise anyway,” Dr. Vinjé said. “And that’s how the virus gets onboard. Then it lands on handrails and doorknobs, and the transmission continues.”
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  • regardless of the origin, once onboard, the illness spreads widely. He says the reason is failure to clean restrooms properly.
Casey Finnerty

Cruise Ship Illness: Why Are Ships So Prone to Norovirus Outbreaks? - 0 views

  • The industrial-size servings of food on a cruise ship with hundreds of passengers can be particularly worrisome, since once the virus enters the food it can spread rapidly. Food can also get more easily contaminated with the virus if it sits out for several hours, as is often the case with buffet-style meals.
  • And so many people being in one place eases the virus's spread. "In close quarters it doesn't get away, everything's concentrated," Zimring says.
  • The best defense against gastrointestinal disease, Zimring says, is to wash and sanitize your hands constantly
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  • avoid buffet meals entirely
  • try to be the first person there at the buffet
  • Don't eat tepid food.
Casey Finnerty

Conventional and unconventional mechanisms for capping viral mRNA: EBSCOhost - 0 views

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    Nice review of 5' methylguanosine capping mechanisms found by Jorge.
Casey Finnerty

Obama Presses Leaders to Speed Ebola Response - NYTimes.com - 0 views

  • On Tuesday night, administration officials said the Pentagon would ask Congress to redirect $500 million from existing Defense Department funds to fight Ebola. The money is in addition to $500 million the Pentagon requested last week in redirected funds for both Iraq and Ebola.
Casey Finnerty

Laboratory Test Support for Ebola Patients Within a High-Containment Facility - 0 views

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    A fascinating description of the treatment facility at Emory University that was used to care for the Americans returning from Africa with Ebola virus infections.
Casey Finnerty

Norovirus Translation Requires an Interaction between the C Terminus of the Genome-link... - 75 views

  • human noroviruses have yet to be cultivated in the laboratory (18).
  • Our understanding of norovirus biology has been greatly enhanced by the discovery of murine norovirus (MNV) in 2003 (19),
  • Previous reports have also highlighted a potential association of the norovirus VPg protein with components of the eIF3 complex (28) and eIF4E, PABP, and eIF4G as well as the ribosomal protein S6 (29), although the functional relevance of these interactions has yet to be demonstrated. Here we describe the proteomic characterization of the murine norovirus translation initiation factor complex, demonstrating that VPg associates directly with the core components of the eIF4F complex and PABP. We further demonstrate that the interaction between eIF4G and VPg is essential for norovirus translation. Furthermore, we demonstrate that eIF4G is required for efficient virus replication in cell culture.
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  • It is worth noting, however, that in addition to disrupting electrostatic interactions, increasing sodium chloride concentrations stabilize hydrophobic interactions.
  • the first step in translation is the binding of the initiation factor eIF4E, a component of the eIF4F complex, to the 5′ cap structure.
  • eIF4F is a complex of three initiation factors; eIF4E is the cap-binding protein, eIF4A functions as an RNA helicase, and eIF4G acts as a scaffold to bridge the mRNA to the 40 S ribosomal subunit via its interaction with eIF3 (3).
  • 14 individual point mutations in VPg were introduced into the MNV infectious clone,
  • the effect of mutations on the ability of VPg to associate with initiation factors was assessed by the enrichment of the eIF4F complex using m7GTP-Sepharose followed by Western blotting for VPg.
  • the mutations V115A, D116A, and F123A altered the levels of infectious virus produced;
  • the mutants V115A, D116A, and F123A showed a consistently reduced interaction with eIF4F (Fig. 4).
  • The degree to which the mutation affected virus recovery appeared to correlate with the ability of VPg to be co-purified with initiation factors (Fig. 4).
  • These data would indicate that the disordered C terminus of the norovirus VPg protein contains amino acids involved in the interaction with eIF4G.
  • eIF4GI
    • Casey Finnerty
       
      eIF4GI and eIF4GII are related forms of eIF4G.
  • This approach was used in place of authentic infection to enable the use of the F123A VPg mutant as a specificity control as this cDNA clone does not produce infectious virus (Fig. 4).
    • Casey Finnerty
       
      Throughout the paper, the authors have included helpful explanatory text like this.
  • These data indicate that the VPg binding site lies within residues 654–1131 of eIF4GI, a region known to contain both eIF4A and eIF3 binding sites (7).
    • Casey Finnerty
       
      Couldn't the binding site be narrowed further to 675-1131?
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    Journal Article for our Week 4 discussion.
Casey Finnerty

What We're Afraid to Say About Ebola - NYTimes.com - 9 views

  • The first possibility is that the Ebola virus spreads from West Africa to megacities in other regions of the developing world.
  • The second possibility is one that virologists are loath to discuss openly but are definitely considering in private: that an Ebola virus could mutate to become transmissible through the air.
  • The current Ebola virus’s hyper-evolution is unprecedented; there has been more human-to-human transmission in the past four months than most likely occurred in the last 500 to 1,000 years. Each new infection represents trillions of throws of the genetic dice.
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  • In 2012, a team of Canadian researchers proved that Ebola Zaire, the same virus that is causing the West Africa outbreak, could be transmitted by the respiratory route from pigs to monkeys, both of whose lungs are very similar to those of humans.
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