Immunocytometry and immunohistochemistry. For immunocytometry, HEK293 cell lines were grown on glass-base dishes (Asahi Techno Glass, Tokyo, Japan). Transfected <a href="http://www.pyrene-azide-2.com/it-should-be-noted-that-der-p-modified.html">3X flag tag</a> were fixed in 4% paraformaldehyde/PBS f