175453-08-4 RNA purification and quantitative RT PCR analysis To

To construct the vector expressing ACE, a BamHI fragment from pUC19 containing a full-length ACE cDNA was inserted into the corresponding site of GCDNsap followed by insertion of the internal ribosomal entry site/puror into the vector. The resultant vector was named pGCDNsap-ACE-I/P. Abstract Keywor