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The purpose of this FOA is to establish a center to coordinate and analyze single cell and other molecular data sets generated by SCORCH and other NIDA-funded HIV and substance use disorder projects and to make the data findable, accessible, interoperable, and reusable (FAIR) to enable secondary analyses by the scientific community. The SCORCH Data Center will coordinate, curate, analyze, and provide public access to single cell SCORCH datasets and other NIDA-generated molecular HIV/SUD data. The SCORCH Data Center will also be responsible for integrating the efforts of all funded components of the NIDA SCORCH program and serve as a community-wide nexus for single cell protocols, data, assay and data standards, and other resources generated by the program. The SCORCH Data Center and NIH staff will need to work closely together to accomplish these goals. Applications that are not responsive to this FOA will not be reviewed.  To be responsive to this FOA, projects should be framed to answer one or more vexing questions about persistent HIV infection in the brain.  In addition, the major thrust of the proposed project MUST: propose to coordinate and analyze single cell and other molecular data sets generated by SCORCH and other NIDA-funded HIV and substance use disorder projects. propose to make this data findable, accessible, interoperable, and reusable (FAIR) to enable secondary analyses by the scientific community.

The purpose of this FOA is to support generation of single cell RNA-sequencing datasets for at least one brain region relevant to persistent HIV infection and opioid use disorder. Applications that are not responsive to this FOA will be returned without review. To be responsive to this FOA, proposed projects should be framed to answer one or more vexing questions about persistent HIV infection in the CNS. The major thrust of the proposed project also MUST: exploit single nucleus or single cell transcriptomic assays with the goal of identifying the types of cells within at least one NIDA-relevant brain region (e.g. PFC, NAc, VTA, striatum, insula, or other appropriately justified region) and how the cell types and individual cells within that region differ from one another in terms of gene expression. focus on human post-mortem brain tissue from controls, individuals with chronic opioid exposure, HIV-infected individuals, and HIV-infected individuals with chronic opioid exposure. propose to detect HIV proteins or RNA or DNA within the single cells or nuclei to be assayed.