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Fish are often used in environmental monitoring, embryo research, tumor research and others. Among them, zebrafish is the most commonly used. The zebrafish is a common tropical fish, which is native to South Asia. The zebrafish have a slender body with an adult length of 3~4cm, and they do not require high water quality. About 3 months after hatching, the fish reach sexual maturity, and mature fish can lay eggs every few days. The eggs are fertilized in vitro and develop in vitro. The embryo development is synchronous and fast, and the embryo body is transparent.