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A transgene is an artificial gene whose structure contains all the critical elements for gene expression. To create a transgene, recombinant DNA (rDNA) is needed. The process for making rDNA is a "cut and paste" method requiring the use of restriction enzymes and DNA ligase.

Transgenic animal models make the study of biological and chemical compounds intended for human use safer and more efficient. The technology also makes it possible to study the gene function and regulation of the organism

The rapid generation of the mice is done by the transduction of early embryos with adenoviral vectors and lent viral silencing. Such methodologies open up chances of large scale generation of knockdown transgenic mice, which can be used for functional genomics studies. Within just 7 seven weeks, this process enables the production of transgenic mice.

The CRISPR-Cas9 system from bacteria has been applied to genome editing in different species, including Drosophila, elegans , zebrafish , mouse , rat , and also human .