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WHAT IS PROTEINASE K MADE OF? Proteinase K is known enzyme belonging to the class of serine proteases that cleave adjacent to the carboxylic group peptide bonds of aromatic and aliphatic amino acids. It's also exhibited a very broad cleavage specificity. The enzyme was discovered in extracts of the fungus Engyodontium album. HOW DOES PROTEINASE K WORK? In molecular biology, main application of proteinase K is to digest proteins during nucleic acids purification in order to remove protein contaminants: - Proteinase K quickly inactivates nucleases (enzymes that digest nucleic acids) that degrade DNA or RNA molecules during the cleansing. - Proteinase K is activated by calcium. It does not affect the activity of the proteinase, but protects again autolysis, improves thermal stability, and regulates substrate binding capacity. To increase the stability of Proteinase K, thereby enhancing its action, Urea as well as SDS (sodium dodecyl sulfate) or elevated temperature at 37-60C is commonly used. Inactivation of Proteinase K is occurring at temperature above 65C. WHAT IS THE PURPOSE OF PROTEINASES? Proteinase K is used to break down proteins in cell lysates (tissues, cell culture cells) and to release nucleic acids due to its action described above. Proteinase K application purpose: DNA isolation from various tissues; Removal of DNAses and RNAses: Proteinase K and RNases can act together in the lysis buffer to degrades contaminating RNAs and proteins;

Are you looking to start a career in the medical field but don't know where to begin? There are many different paths to choose from when it comes to entering the medical profession. It can be daunting trying to figure out which one is right for you. But with some research, planning, and dedication, you can find your way into this rewarding and fulfilling industry. Here are 8 tips that will help get you started on your journey toward becoming a successful healthcare professional. 1. ONLINE PROGRAMS If you have already decided on a career path and need to obtain additional qualifications or knowledge, there are many online programs available for medical professionals. These courses provide students with the opportunity to learn from home and gain experience in their chosen field without sacrificing the quality of education. You can find an online medical assistant programs, medical biller and coding programs, lab technician training, and more. It's important to research each program and make sure it's accredited before enrolling. While online programs may not offer the same hands-on experience as a traditional school, they can be an affordable and convenient way to get your foot in the door. 2. VOLUNTEERING OR WORKING IN A CLINICAL SETTING Volunteering or working part-time in a clinical setting is an excellent way to gain experience and decide if the medicine is the right profession for you. Even volunteer work can provide invaluable insight into how the medical field works, allowing you to network with professionals, observe procedures and treatment methods, and develop skills from patient interaction. Additionally, any volunteer work or job experience related to healthcare that appears on your resume establishes you as a serious candidate for a career in medicine. While you are likely to find many volunteer opportunities, be sure to do your research and only take positions in reputable organizations.

After you've had surgery, it's normal to feel a little anxious about the recovery process. But there are some things you can do to help make sure everything goes smoothly. Here are four important pieces of postoperative advice that will help you heal quickly and avoid any complications. Follow these tips and you'll be on your way to a full recovery in no time. FOLLOW YOUR DOCTOR'S ORDERS No matter how minor the operation, following your doctor's orders after surgery is essential to a speedy and successful recovery. Doing the right things like following a balanced diet, taking the recommended amount of medications, and avoiding strenuous activity can help minimize complications, reduce pain, and speed up healing. Furthermore, consistent follow-up visits with your doctor and timely scheduling of any necessary tests ensure that any post-operative problems are addressed in their early stages when they are easiest to treat. The people behind www.alamoplasticsurgery.com note that failing to take your doctor's instructions seriously can have a significant impact on your overall recovery time, jeopardize the long-term outcome of your treatment, or even lead to additional procedures. To ensure you make the most out of your surgery experience - listen to what your doctor has to say! GET PLENTY OF REST Getting a full night's rest after the surgery is key to a successful recovery. During recovery, your body is working overtime to heal and repair itself, so by getting plenty of rest, you'll give it the best opportunity to do this. Not only that - research has shown that getting lots of sleep may help support important bodily functions related to healing such as immune response and inflammation control. Listen to your doctor's advice post-surgery and make sure you get enough sleep - providing your body with the downtime it needs will ultimately speed up the healing process and help ensure you get back on your feet soon. When it comes to recovering from surgery,

Injuries can be devastating, not only physically but emotionally as well. It's hard to adjust to the new reality of your life after you have been injured, and it's even harder when you feel like no one understands. But there are certain things that every injured person needs to hear in order to keep going. These six important things to know to make things easier: 1. YOU ARE NOT ALONE Everyone has gone through something similar, so don't feel like you have to carry this burden all by yourself. Reach out for help and support from family, friends, and professionals who can understand what you're going through and provide guidance as needed. In addition, there are many support groups available that can help you connect with others in similar situations. Also, don't be afraid to seek out therapy or counseling if you need it. For example, if you are going through a traumatic experience, PTSD counseling can help you process your feelings. 2. IT'S OKAY NOT TO BE OKAY It is understandable that you may feel overwhelmed or angry. You are allowed to have these emotions and it does not make you weak or ungrateful for all of the help that you have received. Allowing yourself to express and process these emotions in a healthy way is important for your mental health. Find someone who will listen and allow yourself the time and space to be honest about how you feel. In addition, it's important to take time for yourself and find healthy ways to cope such as exercise, art or music therapy, or simply engaging in activities that make you happy.

The NHS on Sunday (January 30) expanded its Covid-19 vaccination programme to include vulnerable children aged five to 11 years. Eligible children include those with diabetes, immunosuppression, learning disabilities, and other conditions as outlined by the UK Health Security Agency in the Green Book. Additionally, children living with someone immunosuppressed will be eligible to get the first dose of the Covid-19 vaccine in line with advice issued by the Joint Committee on Vaccination and Immunisation (JCVI). According to the NHS, there are around 500,000 eligible children in the latest cohort. GP and deputy lead for NHS vaccination programme Dr Nikki Kanani, said: "We know vaccines give significant protection against severe illness from Covid - including the omicron variant, so it is important that our youngest and most at-risk get protected.

One of the long-standing medical mysteries is the diagnosis of diabetes in majority of patients suffering from high blood pressure. The reason for this had been unknown. But now, an international team of universities have made a breakthrough. The research, published online in 'Circulation Research', involved contributions from collaborating scientists in Brazil, Germany, Lithuania, and Serbia, as well as the UK and New Zealand. The important new discovery has shown that a small protein cell glucagon-like peptide-1 (GLP-1) couples the body's control of blood sugar and blood pressure. Professor Julian Paton, a senior author, and director of Manaaki Manawa, The Centre for Heart Research at the University of Auckland, said: "We've known for a long time that hypertension and diabetes are inextricably linked and have finally discovered the reason, which will now inform new treatment strategies." GLP-1 is released from the wall of the gut after eating and acts to stimulate insulin from the pancreas to control blood sugar levels. This was known but what has now been unearthed is that GLP-1 also stimulates a small sensory organ called the carotid body located in the neck.

Britain has started counting possible Covid-19 reinfections in its daily coronavirus data, changing its approach to reflect the increased number of people catching the disease for a second time as the Omicron variant predominates. The UK Health Security Agency (UKHSA) added around 840,000 cases to the cumulative total, taking it to 17.3 million coronavirus infections reported. Britain's daily Covid statistics previously would only count people who had tested positive for the first time to avoid double-counting people who had received multiple positive test results for the same infection. However, with variants such as Omicron leading to an increase in reinfections, the UKHSA said it would change its method to treat positive tests as separate infections if there was at least 90 days between test results. The change took effect on Monday (January 31). "Reinfection remained at very low levels until the start of the Omicron wave. It is right that our daily reporting processes reflect how the virus has changed," said Steven Riley, UKHSA's Director General of Data and Analytics.

The NHS is going to roll out a revolutionary 30-minute treatment for advanced womb cancer for women across England. The drug, called Dostarlimab, would benefit around 100 women with advanced and often incurable endometrial cancer every year after the NHS agreed early access to the treatment through the Cancer Drugs Fund. It works by attaching to a specific protein on the surface of the cancer cells, helping the immune system to detect and attack it. The treatment takes just 30 minutes to administer through the blood stream every three weeks over a 12-week period. The move is in line with the NHS Long Term Plan which aims to provide the latest cutting-edge treatments and therapies for patients.

NHS chief executive Amanda Pritchard today (March 1) announced a new campaign to promote early detection of cancer, when it is easier to treat. The campaign, which would run across TV, radio, and social media from Wednesday (March 2), is the first to focus on tackling the fear of cancer rather thanspecific symptoms. It is in line with the NHS Long Term Plan's commitment to increase cancer detection at an early stage by 2028. As per the NHS figures, the number of people getting checked for cancer increased by over half a million between December 2020 and December 2021. Speaking ahead of the launch, Pritchard, said: "We know that the prospect of a cancer diagnosis can be daunting for people and that is exactly why we are launching this potentially lifesaving campaign - we want to allay people's fear about cancer and encourage them to get checked without delay.

The Royal Pharmaceutical Society (RPS) has confirmed that the recall of blood pressure drugs amlodipine and olmesartan by the Food and Drug Administration is only for the US market and will not have any impact in the UK. MHRA also clarified that the manufacturer of these drugs, Macleod Pharmaceuticals, does not supply amlodipine medicines in the UK. It was published online in both the Daily Record and Daily Express on the morning of Monday, March 21, that the drugs had been recalled in the US by the FDA due to deviations from standard manufacturing protocols by the manufacturer. RPS intervened to ensure the articles were edited to make clear that this is not an issue in the UK after confirmation from the MHRA. Amlodipine tablets from Macleod Pharmaceuticals aren't available for wholesale distribution in the UK.

Superdrug has unveiled its new fertility test service to help women to easily access information about their own fertility and enables them to take greater control of their reproductive health. The service comprises a Superdrug online doctor consultation and personalised fertility test recommendations to help women make more informed decisions about the options available to them. Superdrug's fertility test initial consultation requires customers to fill out a quick online questionnaire. This is then reviewed by Superdrug's team of online doctors, who will work with the patients to identify which fertility tests are best suited to the individual. The new service also sees Superdrug introduce three new fertility blood tests to its portfolio, ensuring greater choice and accessibility. Dr Sara Kayat, Superdrug's medical ambassador comments, "For women that have been trying to get pregnant and not succeeding, or for those who are just curious about their fertility, easy access to blood tests may help provide answers and signpost them in the right direction."

The National Institute for Health and Care Excellence (NICE)'s independent committee has called for more research to ensure liposuction for treatment of chronic lipoedema is safe and effective enough for use on the NHS. Lipoedema is more prevalent in women and very rarely affects men. It is characterised by an abnormal, usually symmetrical, accumulation of fat in the legs, hips, buttocks, and occasionally arms. It is a separate condition to obesity and lymphoedema. The cause of lipoedema is unknown, but hormonal changes, weight gain and genetics are thought to be involved. The size and shape of legs, and the resultant mobility issues and pain, can have a profoundly negative effect on quality of life, and physical and mental health. The interventional procedures advisory committee reviewed evidence from several sources, including eight before-and-after studies. The committee was informed that several different liposuction techniques are used and that they may have different safety and efficacy profiles.

Type 1 diabetes patients can monitor their glucose with the newly introduced flash glucose monitors by the NHS. The wearable gadgets with the size of a £2 coin, have a sensor that easily sits on the arm, allowing patients to check their glucose levels with a one-second scan. Eligible patients are currently able to access the monitors on prescription from their local GP or diabetes team, helping them to better manage their blood sugar levels. Everyone living with type 1 diabetes will be eligible for lifechanging flash glucose monitors on the NHS. The NHS Long Term Plan has already helped almost three in five people with Type 1 diabetes to access the monitors that allow people to check their glucose levels more easily and regularly. The change, confirmed today by the National Institute for Health and Care Excellence (NICE), means that everyone in England with the condition will be able to benefit from the convenient technology.

The Department of Health and Social Care (DHSC) has announced 30 pence increase in prescription charges from £9.35 to £9.65 for each medicine or appliance dispensed. Board Chair for England at RPS, Thorrun Govind commented: "This is a kick in the teeth for people in England who are already struggling with rising bills and food prices. "Patients groups have warned that people are not collecting prescription medicines due to cost and pharmacists are seeing this worrying trend first-hand. "A Government impact assessment noted the risk of adverse effects of people not taking their medicines, resulting in future health problems for the individual, potential hospital admissions, and a subsequent cost to the NHS. "This decision seems to prioritise revenue generation over ill-health prevention and undermines the principle of an NHS free at the point of use.

A new, independent search engine has launched called Vitaminology which enables the search, discovery and comparison of vitamins and supplements to empower consumers to make more informed choices and find the right products for their personal health goals and requirements. The company has set out to reduce confusion in this market through choice and high-quality information as well as access to one-to-one consultations with accredited nutritional therapists. By offering the complete package of advice, resources and products Vitaminology helps people to lead healthier lives. Mike Murphy, who is chief nutritionist for Vitaminology, comments, "This innovative platform has a powerful comparison and filtering feature that helps to simplify the choices available for consumers so they can make better self-care decisions. This is supported by access to video consultations with nutritional therapists as well as a comprehensive library of ingredient profiles, articles clustered around health conditions and unique recipes to support wellness."

Sensodyne, the No.1 dentist recommended toothpaste brand for sensitive teeth is relaunching its Sensitivity & Gum range with an improved formula and revitalised packaging. Available in two variants - Mint and Whitening - the range's dual action formula is clinically proven to protect sensitive teeth and improve gum health. Based on a sample survey from across the GB, of those that suffer from gum conditions, 71% claim to also have sensitive teeth[1]. Figures in 2022 showed that 90% of dental practices were unable to take on new adult NHS patients[2], making it challenging for adults to get support or advice from their dentist on these concerns. This shows a clear market need for a multi-purpose product which offers dual action to for sensitive teeth and gum problems. The relaunched Sensitivity & Gum range features a new Micro Foam formula which targets and removes plaque bacteria. With twice daily brushing, the formulation reaches the areas in between the teeth and along the gumline. It also builds a protective layer over sensitive areas of the teeth for daily repair, and the mint flavour gives a soothing sensation. For consumers looking for whitening benefits, the Sensitivity & Gum Whitening formulation not only helps protect sensitive teeth but helps to remove stains for whiter looking teeth resulting in a healthy and confident smile.

When you visit a hospital, one of the last things that come to mind is worrying about legal aspects and what rights you might have - or lack. But these issues and your understanding of them are more important than ever. Knowing exactly what hospitals can legally and cannot do to you is an essential part of ensuring your protection as a patient when going in for care. In this blog post, we'll break down the legal details behind being treated at a hospital so that you can feel more confident when making healthcare decisions. Let's dive into the legal aspect of visiting a hospital, outline what hospitals can and cannot do to us, discover our rights as patients, and review any other considerations worth keeping in mind! OVERVIEW OF MEDICAL MALPRACTICE LAW AND HOW IT RELATES TO VISITING A HOSPITAL When going to a hospital, concerned patients and their families should be aware of the legal risks posed in medical malpractice cases. These potential issues can have life-altering consequences if misdiagnoses, wrong medication administration, or improper treatment occurs. Now, whether you seek damages for your injuries at Staten Island University Hospital or in a different hospital in your hometown, it is important for visitors to understand that the law has set forth regulations regarding acceptable standards of care expected by our nation's hospitals. Those regulations can include procedures of qualified professionals that protect patients from financial exploitation and physical or emotional distress caused by medical negligence. Understanding how this information applies to you will help you protect yourself legally if your rights are violated while visiting a hospital.

The Association of the British Pharmaceutical Industry (ABPI) has suspended the membership of Novo Nordisk for two years due to serious breaches of its Code of Practice. The action was taken by the ABPI Board following an investigation and appeals process conducted by the Prescription Medicines Code of Practice Authority (PMCPA) which found Novo Nordisk to be in breach of the ABPI Code of Practice, including Clause 2 which deals with actions "likely to bring discredit on, or reduce confidence in, the pharmaceutical industry". Having considered the case and requesting a further detailed audit of Novo Nordisk's compliance processes, the ABPI Board decided to suspend Novo Nordisk from ABPI membership. Susan Rienow, President-Elect, ABPI, said: "The ABPI Board has carefully considered the PMCPA's case report into Novo Nordisk and the subsequent audit of their compliance procedures. "The Board expressed significant concern about Novo Nordisk's compliance activities and the very serious issues identified. As a result, the ABPI Board have taken the decision to suspend Novo Nordisk from ABPI membership.

The new 'How to Involve and Engage Patients on Digital Health Tech Innovation' learning module has been created specifically to support the development and delivery of patient-centric technologies, at a time of critical digital transformation in the NHS. The foundation level module will be freely available at www.orcha-digitalhealthacademy.com and on the Health Education England NHS Learning Hub (learninghub.nhs.uk). No training previously exists on conducting effective patient and public involvement and engagement (PPIE), leading to wasted resource on unsuitable technologies at a time when the healthcare system simply cannot afford it. The module aims to educate innovators who are creating new technology, and the clinicians who are prescribing these solutions. Crucially, the module also provides valuable support to the 500 NHS clinicians who are on the Clinical Entrepreneur Programme. The module is an introduction to the first evidence-based framework for PPIE, launched by the University of Plymouth, the AHSN Network (the national voice of the 15 academic health science networks in England) and Boehringer Ingelheim UK & Ireland. It helps to fast-track learning for the EnACT principles described in the framework, outlining how to involve patients in product innovation and critical issues such as data privacy, intellectual property, inclusivity, reimbursement, useability, and recruitment of patients.

The Association of the British Pharmaceutical Industry (ABPI) has welcomed the new research guidance published by NHS England for the integrated care systems. The guidance is said to reflect the benefits of delivering research, for patients, staff and healthcare system performance, including the economic benefits for the NHS. Jennifer Harris, ABPI's Director of Research Policy, said: "This guidance is clear on what good research practice looks like and it's great to see it out and available for the NHS to use. "A research-active NHS delivers huge value to patients, staff and the system itself. We hope that putting this guidance into action will help Integrated Care Systems realise the huge benefits that research can offer, and help make the UK once again a destination of choice for developing the medical breakthroughs people need."