equation(1)F0/F=1+Ksv[Q]F0/F=1+Ksv[Q]where F0 and F are the respective fluorescence intensities in the absence and presence of acrylamide [Q], and Ksv <a href="http://www.trh-precursor-peptide.com/fluorescence-emission-spectra-were-used-to-assess-the-microenvironmental-changes.html">PMSF</a> the Ste