68858-20-8 Large scale purification of GST GFP cultured overnigh

Coimmunoprecipitation. Cleared cell lysates were mixed with 1 μg of anti-myc (9E10) mouse monoclonal antibody, reacted with 40 μl of packed protein G–agarose (Upstate) and then washed five times with ice-cold PBS (pH 7.4). Captured proteins were analyzed by SDS–PAGE and Western blotting. Protea