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Preparation, Usage and Precautions of Agar Medium Agar is a polysaccharide that is derived from plants, mostly marine algae. Agar is mostly employed as a coagulant in microbiological medium. Agar is added to fluid media at 0.5-2g/L, semi-solid media at 3.5-4g/L, plant tissue media at 5-10g/L, and solid media at 12-15g/L.