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Gold nanoparticles have attracted great interests in the fields of biological and medical applications in past few years. Comparing with quantum dots and other materials, gold nanoparticles have been investigated and utilized in several biotechnology applications, such as sensory probes, drug delivery, and therapy techniques. In addition, gold nanoparticles can be excited by light at Near-IR absorbing, which makes them to be the next generation contrast agents for diagnostic and phototherapeutic applications, such as two-photon luminescence imaging, light-scattering imaging, surface-enhanced Raman scattering, and photothermal therapy.