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Kylie John

Drought-induced injury is associated with hormonal alteration in Kentucky bluegrass - PMC - 0 views

www.ncbi.nlm.nih.gov/...PMC6768267

HSR-2025 HSR botany hormones lawngrass

shared by Kylie John on 20 Sep 24 - No Cached
  • Mature KBG (cv. ‘Wildhorse’) plugs (10 cm diameter, 5 cm deep)
  • The grass was transplanted into pots (15 cm diameter, 15 cm deep, with 8 holes on the bottom) filled with either top soil and sand mixture (2:1, v/v). A piece of plastic screen was placed in the bottom of the pot to prevent soil from leaching.
  • at optimum conditions (mean±SD) at 22 ± 0.8/16 ± 0.6°C (day/night), 70%±8% relative humidity, PAR at 400 ± 9 µmol m−2 s−1 and 12-h photoperiod. Nitrogen was applied at 2 g m−2 (from 28–8-18 complete fertilizer with micronutrients) at transplanting and then 1 g m−2 biweekly on all treatments until the end of the trial.
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  • trimmed at 7 cm and irrigated two times a week to field capacity.
  • Six weeks after transplanting
  • For drought stress treatment, the amount of irrigation water was determined based on evapotranspiration (ET) loss by weighing the pots every other day and the irrigation was provided to compensate 30% to 50% ET loss. ET was determined by weighing the pots.
  • VWC was reduced from 34.6% to 27.9% between day 0 and day 7, from 27.9% to 23.4% between day 7 and day 14, from 23.4%% to 14.5% between day 14 to day 21, and from 14.5% to 5.1% between day 21 and day 28.
  • Leaf chlorophyll was extracted with acetone and measured using a spectrophotometer
  • Leaf photosynthetic rate and stomatal conductance were measured using a portable photosynthetic system
  • turf quality started to decline at day 7 due to drought stress.
  • Drought stress reduced turf quality rating by 14.8% relative to the control at the end of drought stress (28 d).
  • The electrolyte leakage increased gradually from day 7 through day 21 due to drought stress.
  • Drought stress increased electrolyte leakage by 35.6% at day 14, 95.7% at day 21, and 105.4% at day 28 when compared to the control.
  • chlorophyll began to decline after day 7 due to drought stress
  • educed chlorophyll content by 39.0% relative to the control.
  • photosynthetic rate due to drought stress was observed as early as day 7
  • Stomatal conductance decline was first observed at day 4 of drought stress
  • day 28 when drought stress reduced stomatal conductance by 84.4%
  • The ZR level declined gradually from day 14 through day 28 due to drought stress. Drought stress reduced ZR by 28.5% at day 14, 35.1% at day 21, and 59.1% at day 28 relative to the control.
  • 1 week after ZR did. At the end of drought stress (day 28), drought stress reduced iPA by 50.4% relative to the control.
  • Drought stress reduced leaf IAA by 14.3% and 26.7% at day 21 and day 28, respectively, when compared to the control.
  • The ABA started to accumulate after day 4 of drought stress and the difference between drought stress and the control was observed as early as 7 d of drought stress
  • The ABA content gradually increased from day 7 through day 28. Drought stress increased leaf ABA by 23.6% at day 7, 30.7% at day 14, 87.6% at day 21, and 108.5% at day 28 relative to the control.
  • drought stress did not impact leaf GA4 content
  • We found that ABA/CK increased sharply from day 14 through day 28 due to increase in ABA and decline in CK (ZR + iPA) during the same period. At the end of trial (day 28), drought stress induced an increase in leaf ABA/CK ratio by 3.4-fold relative to the control.
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