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In a letter to Khamenei, published by the semi-official Mehr news agency, Rafsanjani said tens of millions of Iranians had witnessed "mis-statements and fabrications" during the debate."I am expecting you to resolve this position in order to extinguish the fire, whose smoke can be seen in the atmosphere, and to foil dangerous plots to take action," said Rafsanjani.

Meanwhile, central Tehran saw chaotic scenes for a second day when supporters of Mousavi – many of them young women – flocked in their tens of thousands to another demonstration, shouting anti-Ahmadinejad slogans and waving the green ribbons, banners and posters that have become the symbol of his campaign. A "human chain" rally on Monday night was likened by many to the events that shook Tehran before the 1979 Islamic revolution.

His comments were echoed by 14 high-ranking clerics from the holy city of Qom, who expressed "deep concern and regret" that Iran's image had been harmed in the debate."Accusing those who were not present at that debate and could not defend themselves is against our religion," they said in a statement also published by Mehr.

Previous Mousavi rallies had to be cancelled at the last minute because permission to use large venues was suddenly withdrawn by the authorities. On Sunday a rally at Karaj outside Tehran could not be held because the electricity supply to the public address system failed.

Last night, in the last of six televised debates, Ahmadinejad clashed over the economy with the other conservative candidate, a former Revolutionary Guards commander, Mohsen Rezaei.Ahmadinejad insisted that over the last four years he had slashed inflation to 15%, but Rezaei, an economist, said the true figure was 25%.

The fourth candidate, reformist cleric Mehdi Karoubi, dismissed as "psychological warfare" rumours that he might withdraw from the race to boost Mousavi's chances.

Ahmadinejad remained defiant towards the outside world, especially over the nuclear issue that has done so much to isolate Iran.At a campaign event in the Caspian Sea province of Mazenderan he said: "Let the world know that if the Iranian nation should re-elect this small servant, he would go forward in the world arena with the nation's authority and would not withdraw an iota from the nation's rights."

Earlier on Tuesday, the opposition was told by Iran's Guardian Council, the legislative body for elections, that the presidential election result would not be annulled. Guardian Council spokesman Abbas Ali Kadkhoda'i said there had been "no major fraud or breach in the election". But Iran's Supreme Leader Ayatollah Ali Khamenei later agreed to extend by five days the amount of time allowed to examine complaints of electoral fraud.

Mohsen Rezai, who is a former leader of the Revolutionary Guards, said he had withdrawn his complaints about the vote in the interests of Iran's national security. "I see it as my responsibility to encourage myself and others to control the current situation," Mr Rezai was quoted as saying in a letter to the Guardian Council.

The remaining two candidates have called for the elections to be re-run, amid claims of vote tampering. Opposition candidate Mehdi Karroubi has urged Iranians to mourn for dead protesters on Thursday. His call echoed an earlier one from cleric Grand Ayatollah Hossein Ali Montazeri for three days of national mourning for those killed in the street protests.