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Emma Chowdhury

Forensic DNA technology: A powerful tool for judicial reform | Science and Technology, ... - 0 views

  • Recent advances in forensic DNA testing are now paving the way for reforming the manner by which cases are resolved in courts of law through the way suspected offenders are apprehended during a criminal investigation. Firstly, the availability of new markers which are more variable across different populations, adds to the increased power of discrimination once more genetic markers are used. From the time when DNA testing only involved seven to nine genetic markers to evaluate if crime scene evidence matches a suspect’s profile, as well as to determine relationships, to the current battery of 21 autosomal markers and 23 male-specific markers, the capacity of DNA profiling to differentiate individuals has increased significantly. The use of automated and expert systems for large-scale analysis has also been found to reduce manual errors and to increase output per unit time. The use of several dyes in a single multiplex system provides more information from the same amount of genetic material compared to reactions targeting only one genetic marker but requiring the same amount of DNA that was common in the early 1990s.
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    This is about how they have new technology for forensic DNA sciences, so they make less mistakes and can find the person quickly. For example, they now have automatic systems to test DNA.
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    What is the media type???
Yusuke K

Stem cells research - Portal Brasil - 0 views

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    not sure what the technique used is
Yusuke K

Stem cell cutting-edge tech Media 4 - Yusuke - 0 views

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    Is this your "technique" ? Then you will need a bit more detail on the actual technique. Also please TAG your articles appropriately!
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    Can you tag this properly aswell.
Abraham H

Forensic Techniques [Media 4] - 0 views

  • RFLP is a technique for analyzing the variable lengths of DNA fragments that result from digesting a DNA sample with a special kind of enzyme. This enzyme, a restriction endonuclease, cuts DNA at a specific sequence pattern know as a restriction endonuclease recognition site. The presence or absence of certain recognition sites in a DNA sample generates variable lengths of DNA fragments, which are separated using gel electrophoresis. They are then hybridized with DNA probes that bind to a complementary DNA sequence in the sample. RFLP was one of the first applications of DNA analysis to forensic investigation. With the development of newer, more efficient DNA-analysis techniques, RFLP is not used as much as it once was because it requires relatively large amounts of DNA. In addition, samples degraded by environmental factors, such as dirt or mold, do not work well with RFLP.
  • PCR Analysis Polymerase chain reaction (PCR) is used to make millions of exact copies of DNA from a biological sample. DNA amplification with PCR allows DNA analysis on biological samples as small as a few skin cells. With RFLP, DNA samples would have to be about the size of a quarter. The ability of PCR to amplify such tiny quantities of DNA enables even highly degraded samples to be analyzed. Great care, however, must be taken to prevent contamination with other biological materials during the identifying, collecting, and preserving of a sample.
  • Short tandem repeat (STR) technology is used to evaluate specific regions (loci) within nuclear DNA. Variability in STR regions can be used to distinguish one DNA profile from another. The Federal Bureau of Investigation (FBI) uses a standard set of 13 specific STR regions for CODIS. CODIS is a software program that operates local, state, and national databases of DNA profiles from convicted offenders, unsolved crime scene evidence, and missing persons. The odds that two individuals will have the same 13-loci DNA profile is about one in a billion.
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  • Mitochondrial DNA analysis (mtDNA) can be used to examine the DNA from samples that cannot be analyzed by RFLP or STR. Nuclear DNA must be extracted from samples for use in RFLP, PCR, and STR; however, mtDNA analysis uses DNA extracted from another cellular organelle called a mitochondrion. While older biological samples that lack nucleated cellular material, such as hair, bones, and teeth, cannot be analyzed with STR and RFLP, they can be analyzed with mtDNA. In the investigation of cases that have gone unsolved for many years, mtDNA is extremely valuable. All mothers have the same mitochondrial DNA as their offspring. This is because the mitochondria of each new embryo comes from the mother's egg cell. The father's sperm contributes only nuclear DNA. Comparing the mtDNA profile of unidentified remains with the profile of a potential maternal relative can be an important technique in missing-person investigations.
Eva K

BBC News | TALKING POINT | Should animal cloning be stopped? - 0 views

  • As long as Animal cloning can offer medical benefits it should be investigated.
  • Cloning is stupid, sick, selfish and evil. There is no need for anyone to exploit animals for greed of humans.
  • I have absolutely no problem with animal cloning; it is a necessary step in the evolution of human welfare.
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  • Cloning should not be outlawed as the technology seems to offer new opportunities in medicine.
  • Keep on cloning, practice makes perfect
  • There needs to be a purpose for cloning. Unless animals that are endangered are cloned then it's a waste of time.
Inga V.

Brazil hopes to save species by cloning them | MNN - 1 views

  • some Brazilian biologists are pushing a more controversial idea: They want to save endangered species by cloning them.
  • The project is still in its infancy,
  • and no cloned jaguars or tamarins will likely be born anytime soon.
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  • the plan doesn't involve releasing clones into the wild, where they could undermine conservation by weakening their species' gene pool. Instead, clones would be used to supplement zoo populations and to assist with captive-breeding programs.
  • 90 percent of cloning attempts failing
  • expensive and highly inefficient,"
  • While cloning endangered species is a relatively new pursuit, Brazilian scientists have been copying beef cattle for more than a decade.
  • "We are still in the phase of developing the technology, so we still don't know if it will be possible to rescue a population in the wild, but we could potentially make it viable again."
  • Brazil hopes to save species by cloning them
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    Brazilian biologists want to save different species by cloning animals. These people hope to save the species that way and put clones into zoo's and other places. They would not put them out into the wild because their they might weaken the species.
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    looking for a TV news broadcast
Kareena M

Forensic News Blog - 3 views

  • In 2012, readers searching for forensic science content now have many more ways to find
  • "The body chemistry of the person who left the fingerprint can tell us some things," said Shaler. "If the suspect is older or younger or a lactating mother, for example."The researchers used a form of physical vapor deposition -- a method that uses a vacuum and allows vaporized materials to condense on a surface creating a thin film. Normally, the deposition process requires exceptionally clean surfaces because any speck of dust or grease on the coated surface shows up as a deformity. However, with fingerprints, the point is to have the surface material's ridges and valleys -- topography -- show up on the new surface so analysts can read them using an optical device without the necessity of chemical development or microscopy."This approach allows us to look at the topography better and to look at the chemistry later," said Shaler. "We wouldn't have thought of this by ourselves, but we could do it together."One benefit of this approach would be the ability to retrieve fingerprints off fragments from incendiary or explosive devices and still be able to analyze the chemicals used in the device.The specific method used is a conformal-evaporated-film-by-rotation technique developed to create highly accurate copies of biological templates such as insect eyes or butterfly wings. Both are surfaces that have nanoscale variations."It is a very simple process," said Lakhtakia. "And fingerprints are not nanoscale objects, so the conformal coating is applied to something big by nanotechnology standards."
  • Investigators have found new DNA evidence in the murder of Peggy Hettrick, a case that was considered closed until genetic evidence freed a man who spent 10 years in prison, according to Colorado Attorney General John Suthers.The "touch DNA" tests weren't available in the late 1990s. Timothy Masters was convicted of murder in Hettrick's death in 1999, but his conviction was overturned in 2008 after defense lawyers used advanced DNA testing to uncover evidence suggesting a different suspect.The new evidence was taken from Hettrick's clothing. "We have done 'touch DNA,' and I think it has moved the ball forward. We will know more in the future," Suthers said. He wouldn't say whose DNA was found or identify the clothing on which it was found.Masters has not been exonerated in the case and remains a suspect."While we are not in a position to exonerate Tim at this time, I emphasize that he is presumed innocent and is no more a suspect than a variety of other people," Suthers said.
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  • It's already difficult to obtain good fingerprints at a crime scene. In most cases, mostly partials will be found. In cases where there was some type of explosion or fire, other methods like this chemical method could be very helpful not just in recovering the print but also to find out some information on who the suspect could be.
  • relevant material.  Organized blogs devoted to forensic science have appeared that post both links and original content.  Organizations have twitter accounts and Facebook pages that make reaching readers much easier than in RSS days.
  • As you may know, your skin has "normal flora" bacteria living on it. This bacteria is beneficial to your health, but may also be used to identify you, according to emerging research. Scientists at the University of Colorado at Boulder are developing a method to identify individuals based on the unique bacteria found on their hands. When the amount of human DNA is too small to detect, bacterial DNA could be used in its place. Bacteria can be collected directly from hands or even touched surfaces and the DNA can be sequenced just like human DNA. The scientists found that very few bacteria were shared among test subjects. Even identical twins have different colonies of bacteria on their hands! Bacteria remain on our hands no matter how many times we wash them, so why not use them?
  • ..."Each one of us leaves a unique trail of bugs behind as we travel through our daily lives," said Fierer, an assistant professor in CU-Boulder's ecology and evolutionary biology department.....unless there is blood, tissue, semen or saliva on an object, it's often difficult to obtain sufficient human DNA for forensic identification, said Fierer. But given the abundance of bacterial cells on the skin surface, it may be easier to recover bacterial DNA than human DNA from touched surfaces, they said. "Our technique could provide another independent line of evidence."...The new technique would even be useful for identifying objects touched by identical twins, since they share identical DNA but they have different bacterial communities on their hands.
  • "On Wednesday, Human Rights Watch released a report showing that since 1995, only about 20 percent of rape kits, which contain physical evidence obtained from victims, could be confirmed as having been tested in Illinois. More than 4,000 kits had gone untested, the report found."
  • Law-enforcement experts said iPhone technology records a wealth of information that can be tapped more easily than BlackBerry and Droid devices to help police learn where you've been, what you were doing there and whether you've got something to hide.
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    okay but which exact post do you want me to read?
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    "New DNA Evidence May Exonerate Convicted Murderer"
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    not a very strong, relevant post
Avantika B

Japanese Stem Cell Discovery could lead to Human Cloning without the need for Humans | ... - 0 views

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    This seems fine as long as YOU can tell what the author's opinion on the matter is and support that using evidence. Also please check rubric on how to tag your posts correctly
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    I think that the author's opinion is against Animal Cloning because she uses specific quotes from George W. Bush and also presents the danger of the Nazi's gettting hands on the technology. As well as she says,"It is a slippery slope that we should probably not descend too."
Avantika B

Cloned human embryo makes working stem cells : Nature News - 0 views

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    This is good for technique. What is the technique called and can you explain it briefly in your own words? Please tag correctly using the rubric
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    Scientist in NY part of the Stem Cell foundation changed an adult egg cell to the beginning stages using high-tech cloning technology. This resulted in a self-reproducing chain of embryonic stem cells.The techique is basically cloning a human embryo to create a lot of working stem cells. This can be used for tissue therapy and for research.
Kareena M

BBC News - DNA crime-fighting in UK 'lagging behind', experts say - 0 views

  • Cross-border co-operation on terrorism and crime will be compromised unless the UK updates the technology it uses for DNA profiling, experts have warned.
  • Using EU recommended markers Not using recommended markers Dat
  • Experts also say that the "chemistry" that underlies DNA testing kits used by UK forensic science labs is now more than a decade old and that newer, more sensitive systems can obtain results from even low quality samples - improving success rates. Some argue that such information can potentially make or break a case.
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  • UK experts fear that proposals to destroy so-called "second swab" DNA samples could slow down investigations if and when the UK moves over to new markers and chemistries. Currently, when a DNA sample is obtained from a suspect, a first swab is used to generate a profile in the NDNAD and a second sample is placed into storage. Problems could arise when there was a partial match between a crime scene stain processed using the new markers and an old profile in the database generated using six or 10 markers. Up until now, it would have been possible to re-process the DNA from the second swab, allowing investigators to confirm or deny a match using a comparison based on all the new loci. But soon, that will no longer be an option.
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